Comparative analysis of whole genomes and transcriptomes of Microsporum canis from invasive dermatophytosis and tinea capitis
Genomes of strains of the zoophilic dermatophyte Microsporum canis from invasive (disseminated and subcutaneous) and noninvasive (tinea capitis) infections were compared. Especially the disseminated strain showed significant syntenic rearrangements, including multiple translocations and inversions,...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Emerging Microbes and Infections |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2023.2219346 |
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| author | Ruojun Wang Weixia Liu Xiao Liu Zhe Wan G. Sybren de Hoog Ruoyu Li Yinggai Song |
| author_facet | Ruojun Wang Weixia Liu Xiao Liu Zhe Wan G. Sybren de Hoog Ruoyu Li Yinggai Song |
| author_sort | Ruojun Wang |
| collection | DOAJ |
| description | Genomes of strains of the zoophilic dermatophyte Microsporum canis from invasive (disseminated and subcutaneous) and noninvasive (tinea capitis) infections were compared. Especially the disseminated strain showed significant syntenic rearrangements, including multiple translocations and inversions, and numerous SNPs and Indels in comparison to the noninvasive strain. In transcriptome analysis, both invasive strains were enriched for GO pathways related to components of the membrane, iron binding and heme binding, which possibly enables them to invade deeper into dermis and blood vessels. At 37 °C, invasive strains showed gene expression enriched for DNA replication, mismatch repair, N-glycan biosynthesis and ribosome biogenesis. The invasive strains were slightly less susceptible to multiple antifungal agents suggesting that acquired elevated drug resistance might be involved in the refractory disease courses. Patient with disseminated infection failed to respond to a combined antifungal treatment with itraconazole, terbinafine, fluconazole and posaconazole. |
| format | Article |
| id | doaj-art-04f00cd9bb8447c293535dd797cb1a43 |
| institution | Kabale University |
| issn | 2222-1751 |
| language | English |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj-art-04f00cd9bb8447c293535dd797cb1a432025-08-20T03:52:56ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512023-12-0112110.1080/22221751.2023.2219346Comparative analysis of whole genomes and transcriptomes of Microsporum canis from invasive dermatophytosis and tinea capitisRuojun Wang0Weixia Liu1Xiao Liu2Zhe Wan3G. Sybren de Hoog4Ruoyu Li5Yinggai Song6Department of Dermatology and Venerology, Peking University First Hospital, Beijing, People’s Republic of ChinaDepartment of Dermatology and Venerology, Peking University First Hospital, Beijing, People’s Republic of ChinaDepartment of Dermatology and Venerology, Peking University First Hospital, Beijing, People’s Republic of ChinaDepartment of Dermatology and Venerology, Peking University First Hospital, Beijing, People’s Republic of ChinaDepartment of Dermatology and Venerology, Peking University First Hospital, Beijing, People’s Republic of ChinaDepartment of Dermatology and Venerology, Peking University First Hospital, Beijing, People’s Republic of ChinaDepartment of Dermatology and Venerology, Peking University First Hospital, Beijing, People’s Republic of ChinaGenomes of strains of the zoophilic dermatophyte Microsporum canis from invasive (disseminated and subcutaneous) and noninvasive (tinea capitis) infections were compared. Especially the disseminated strain showed significant syntenic rearrangements, including multiple translocations and inversions, and numerous SNPs and Indels in comparison to the noninvasive strain. In transcriptome analysis, both invasive strains were enriched for GO pathways related to components of the membrane, iron binding and heme binding, which possibly enables them to invade deeper into dermis and blood vessels. At 37 °C, invasive strains showed gene expression enriched for DNA replication, mismatch repair, N-glycan biosynthesis and ribosome biogenesis. The invasive strains were slightly less susceptible to multiple antifungal agents suggesting that acquired elevated drug resistance might be involved in the refractory disease courses. Patient with disseminated infection failed to respond to a combined antifungal treatment with itraconazole, terbinafine, fluconazole and posaconazole.https://www.tandfonline.com/doi/10.1080/22221751.2023.2219346DermatophytesMicrosporum canisWGSgenomicstranscriptomics |
| spellingShingle | Ruojun Wang Weixia Liu Xiao Liu Zhe Wan G. Sybren de Hoog Ruoyu Li Yinggai Song Comparative analysis of whole genomes and transcriptomes of Microsporum canis from invasive dermatophytosis and tinea capitis Emerging Microbes and Infections Dermatophytes Microsporum canis WGS genomics transcriptomics |
| title | Comparative analysis of whole genomes and transcriptomes of Microsporum canis from invasive dermatophytosis and tinea capitis |
| title_full | Comparative analysis of whole genomes and transcriptomes of Microsporum canis from invasive dermatophytosis and tinea capitis |
| title_fullStr | Comparative analysis of whole genomes and transcriptomes of Microsporum canis from invasive dermatophytosis and tinea capitis |
| title_full_unstemmed | Comparative analysis of whole genomes and transcriptomes of Microsporum canis from invasive dermatophytosis and tinea capitis |
| title_short | Comparative analysis of whole genomes and transcriptomes of Microsporum canis from invasive dermatophytosis and tinea capitis |
| title_sort | comparative analysis of whole genomes and transcriptomes of microsporum canis from invasive dermatophytosis and tinea capitis |
| topic | Dermatophytes Microsporum canis WGS genomics transcriptomics |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2023.2219346 |
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