Genome-Wide Comparative Analysis of Invertases in the <i>Salicaceae</i> with the Identification of Genes Involved in Catkin Fiber Initiation and Development
Invertase (INV) irreversibly converts sucrose to glucose and fructose during processes such as differentiation and organ development in plants, especially during the development of trichomes. Systematic identification and analysis of INVs in Salicaceae remain limited. Here, <i>INV</i> ge...
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| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-06-01
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| Series: | Current Issues in Molecular Biology |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1467-3045/47/6/423 |
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| Summary: | Invertase (INV) irreversibly converts sucrose to glucose and fructose during processes such as differentiation and organ development in plants, especially during the development of trichomes. Systematic identification and analysis of INVs in Salicaceae remain limited. Here, <i>INV</i> genes in <i>Populus deltoides</i> and <i>Salix suchowensis</i> were investigated, and their chromosomal localization, collinearity, gene structures, cis-regulatory elements, and phylogenetic relationships were comprehensively analyzed. Twenty and seventeen <i>INVs</i> were found, respectively, in <i>P. deltoides</i> and <i>S. suchowensis</i>, most of which were derived from a common ancestor and exhibited similar chromosomal distribution and high collinearity. Orthologs between the two species showed conservation of gene structures and promoter regulatory elements. Multi-species phylogenetic analysis identified an evolutionary clade associated with seed fiber development in <i>P. deltoides</i> and <i>S. suchowensis</i>. Further evaluation of <i>INV</i> expression in female catkins at various stages of seed fiber formation verified the predominance of <i>PdeVINV1</i>, <i>PdeVINV2</i>, <i>PdeVINV3</i>, and <i>PdeVINV4</i> in <i>P. deltoides</i>, as well as <i>SsuVINV1</i> and <i>SsuVINV2</i> in <i>S. suchowensis</i>, during critical phases of catkin fiber differentiation. These genes are likely to have significant regulatory roles in the initiation and development of catkin fiber cells. These findings provide a reference for future functional studies of INVs. |
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| ISSN: | 1467-3037 1467-3045 |