DNMT3B Expression Might Contribute to Abnormal Methylation of RASSF1A in Lager Colorectal Adenomatous Polyps
Background. It is pretty well known that DNA methyltransferases (DNMTs) are actively involved in abnormal cell growth. The goal of the current study is to explore the correlation between DNMT expression and colorectal adenomatous polyps (CAPs). Method. Twenty pairs of CAP samples with a diameter≥10 ...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Gastroenterology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2020/1798729 |
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| author | Xianmei Meng Na Liu Yanbin Jia Kerui Gong Jingjie Zhang Wei Zhang Guo Shao Tong Dang |
| author_facet | Xianmei Meng Na Liu Yanbin Jia Kerui Gong Jingjie Zhang Wei Zhang Guo Shao Tong Dang |
| author_sort | Xianmei Meng |
| collection | DOAJ |
| description | Background. It is pretty well known that DNA methyltransferases (DNMTs) are actively involved in abnormal cell growth. The goal of the current study is to explore the correlation between DNMT expression and colorectal adenomatous polyps (CAPs). Method. Twenty pairs of CAP samples with a diameter≥10 mm and corresponding normal colorectal mucosa (NCM) tissues from patients were used in the present study. The expression levels and activity of DNA methyltransferases (DNMTs) were measured in the CAP tissues. The global methylation and the promoter methylation level of 3 kinds of tumour suppressor gene were detected. Results. mRNA and protein levels of DNMT3B were found to be elevated in the CAP tissues compared with the control tissue. Additionally, the methylation of long interspersed nuclear elements-1 (LINE-1/L1) was decreased in the CAP tissue. Furthermore, methylation of the promoter of a tumour suppressor gene Ras association domain family 1A (RASSF1A) was increased in the CAP tissues, while the mRNA levels of RASSF1A were decreased. Conclusions. These results suggest that the overexpression of DNMT3B may contribute to a role in the genesis of CAPs through the hypomethylation of chromosomes in the whole cell and promoter hypermethylation of RASSF1A. |
| format | Article |
| id | doaj-art-04ebae14d2e0494984ed55cf86f57bea |
| institution | Kabale University |
| issn | 1687-6121 1687-630X |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Gastroenterology Research and Practice |
| spelling | doaj-art-04ebae14d2e0494984ed55cf86f57bea2025-08-20T03:54:15ZengWileyGastroenterology Research and Practice1687-61211687-630X2020-01-01202010.1155/2020/17987291798729DNMT3B Expression Might Contribute to Abnormal Methylation of RASSF1A in Lager Colorectal Adenomatous PolypsXianmei Meng0Na Liu1Yanbin Jia2Kerui Gong3Jingjie Zhang4Wei Zhang5Guo Shao6Tong Dang7Department of Gastroenterology, The Second Affiliated Hospital of Baotou Medical College, Baotou, 014030 Inner Mongolia Autonomous Region, ChinaInner Mongolia Key laboratory of Hypoxic Translational Medicine, Baotou Medical College, Baotou, 104060 Inner Mongolia Autonomous Region, ChinaDepartment of Gastroenterology, The Second Affiliated Hospital of Baotou Medical College, Baotou, 014030 Inner Mongolia Autonomous Region, ChinaDepartment of Oral and Maxillofacial Surgery, University of California San Francisco, San Francisco, 94122 CA, USADepartment of Gastroenterology, The Second Affiliated Hospital of Baotou Medical College, Baotou, 014030 Inner Mongolia Autonomous Region, ChinaDepartment of Gastroenterology, The Second Affiliated Hospital of Baotou Medical College, Baotou, 014030 Inner Mongolia Autonomous Region, ChinaInner Mongolia Key laboratory of Hypoxic Translational Medicine, Baotou Medical College, Baotou, 104060 Inner Mongolia Autonomous Region, ChinaDepartment of Gastroenterology, The Second Affiliated Hospital of Baotou Medical College, Baotou, 014030 Inner Mongolia Autonomous Region, ChinaBackground. It is pretty well known that DNA methyltransferases (DNMTs) are actively involved in abnormal cell growth. The goal of the current study is to explore the correlation between DNMT expression and colorectal adenomatous polyps (CAPs). Method. Twenty pairs of CAP samples with a diameter≥10 mm and corresponding normal colorectal mucosa (NCM) tissues from patients were used in the present study. The expression levels and activity of DNA methyltransferases (DNMTs) were measured in the CAP tissues. The global methylation and the promoter methylation level of 3 kinds of tumour suppressor gene were detected. Results. mRNA and protein levels of DNMT3B were found to be elevated in the CAP tissues compared with the control tissue. Additionally, the methylation of long interspersed nuclear elements-1 (LINE-1/L1) was decreased in the CAP tissue. Furthermore, methylation of the promoter of a tumour suppressor gene Ras association domain family 1A (RASSF1A) was increased in the CAP tissues, while the mRNA levels of RASSF1A were decreased. Conclusions. These results suggest that the overexpression of DNMT3B may contribute to a role in the genesis of CAPs through the hypomethylation of chromosomes in the whole cell and promoter hypermethylation of RASSF1A.http://dx.doi.org/10.1155/2020/1798729 |
| spellingShingle | Xianmei Meng Na Liu Yanbin Jia Kerui Gong Jingjie Zhang Wei Zhang Guo Shao Tong Dang DNMT3B Expression Might Contribute to Abnormal Methylation of RASSF1A in Lager Colorectal Adenomatous Polyps Gastroenterology Research and Practice |
| title | DNMT3B Expression Might Contribute to Abnormal Methylation of RASSF1A in Lager Colorectal Adenomatous Polyps |
| title_full | DNMT3B Expression Might Contribute to Abnormal Methylation of RASSF1A in Lager Colorectal Adenomatous Polyps |
| title_fullStr | DNMT3B Expression Might Contribute to Abnormal Methylation of RASSF1A in Lager Colorectal Adenomatous Polyps |
| title_full_unstemmed | DNMT3B Expression Might Contribute to Abnormal Methylation of RASSF1A in Lager Colorectal Adenomatous Polyps |
| title_short | DNMT3B Expression Might Contribute to Abnormal Methylation of RASSF1A in Lager Colorectal Adenomatous Polyps |
| title_sort | dnmt3b expression might contribute to abnormal methylation of rassf1a in lager colorectal adenomatous polyps |
| url | http://dx.doi.org/10.1155/2020/1798729 |
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