Characterization of katG, inhA, rpoB and pncA in Mycobacterium tuberculosis isolates from MDR-TB risk patients in Thailand
Introduction: Multidrug-resistant tuberculosis (MDR-TB) is commonly found in Thailand especially in the public health region 5, the Western region of Thailand. This study’s aim was to characterize katG, inhA, rpoB and pncA genes in Mycobacterium tuberculosis. Methodology: One hundred strains of...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The Journal of Infection in Developing Countries
2020-03-01
|
| Series: | Journal of Infection in Developing Countries |
| Subjects: | |
| Online Access: | https://jidc.org/index.php/journal/article/view/11974 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850148139494801408 |
|---|---|
| author | Krairerk Suthum Worada Samosornsuk Seksun Samosornsuk |
| author_facet | Krairerk Suthum Worada Samosornsuk Seksun Samosornsuk |
| author_sort | Krairerk Suthum |
| collection | DOAJ |
| description |
Introduction: Multidrug-resistant tuberculosis (MDR-TB) is commonly found in Thailand especially in the public health region 5, the Western region of Thailand. This study’s aim was to characterize katG, inhA, rpoB and pncA genes in Mycobacterium tuberculosis.
Methodology: One hundred strains of Mycobacterium tuberculosis (MTB) were isolated from sputum samples of MDR-TB risk patients in the laboratory of the Office of Disease Prevention and Control 5th Ratchaburi province, Thailand from January to December 2015. Drug susceptibility testing (DST) was performed using a BACTEC MGIT 960 system. Furthermore, the genes katG, inhA, rpoB and pncA were characterized by DNA sequencing.
Results: Of a total of 100 MTB samples which underwent drug susceptibility testing, 42% showed isoniazid (INH) and rifampicin (RIF) resistance, and a further 25% showed INH mono-resistance (25%). The most common gene mutations found using DNA sequencing were katG_Ser315Thr (70%), rpoB_Ser531leu (81%) and pncA_Ile31Thr (84%). The common mutation of pncA_Ile31Thr substitution was detected in 26 of 91 (29%) pyrazinamide (PZA) susceptible isolates.
Conclusion: Using DNA sequencing to screen for gene mutations conferring drug resistance may be feasible and use less time than using DST to detect resistance patterns.
|
| format | Article |
| id | doaj-art-04db25a0df3a45d085c406766ccee7c9 |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2020-03-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-04db25a0df3a45d085c406766ccee7c92025-08-20T02:27:20ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802020-03-01140310.3855/jidc.11974Characterization of katG, inhA, rpoB and pncA in Mycobacterium tuberculosis isolates from MDR-TB risk patients in ThailandKrairerk Suthum0Worada Samosornsuk1Seksun Samosornsuk2The Office of Disease Prevention and Control 5th Ratchaburi, Department of Disease Control, Ministry of Public Health, Ratchaburi Province, ThailandDepartment of Medical Technology, Faculty of Allied Health Sciences, Thammasat University, Pathumthani Province, ThailandDepartment of Medical Technology, Faculty of Allied Health Sciences, Thammasat University, Pathumthani Province, Thailand Introduction: Multidrug-resistant tuberculosis (MDR-TB) is commonly found in Thailand especially in the public health region 5, the Western region of Thailand. This study’s aim was to characterize katG, inhA, rpoB and pncA genes in Mycobacterium tuberculosis. Methodology: One hundred strains of Mycobacterium tuberculosis (MTB) were isolated from sputum samples of MDR-TB risk patients in the laboratory of the Office of Disease Prevention and Control 5th Ratchaburi province, Thailand from January to December 2015. Drug susceptibility testing (DST) was performed using a BACTEC MGIT 960 system. Furthermore, the genes katG, inhA, rpoB and pncA were characterized by DNA sequencing. Results: Of a total of 100 MTB samples which underwent drug susceptibility testing, 42% showed isoniazid (INH) and rifampicin (RIF) resistance, and a further 25% showed INH mono-resistance (25%). The most common gene mutations found using DNA sequencing were katG_Ser315Thr (70%), rpoB_Ser531leu (81%) and pncA_Ile31Thr (84%). The common mutation of pncA_Ile31Thr substitution was detected in 26 of 91 (29%) pyrazinamide (PZA) susceptible isolates. Conclusion: Using DNA sequencing to screen for gene mutations conferring drug resistance may be feasible and use less time than using DST to detect resistance patterns. https://jidc.org/index.php/journal/article/view/11974multidrug-resistant tuberculosisMDR-TBmutationDNA sequencing |
| spellingShingle | Krairerk Suthum Worada Samosornsuk Seksun Samosornsuk Characterization of katG, inhA, rpoB and pncA in Mycobacterium tuberculosis isolates from MDR-TB risk patients in Thailand Journal of Infection in Developing Countries multidrug-resistant tuberculosis MDR-TB mutation DNA sequencing |
| title | Characterization of katG, inhA, rpoB and pncA in Mycobacterium tuberculosis isolates from MDR-TB risk patients in Thailand |
| title_full | Characterization of katG, inhA, rpoB and pncA in Mycobacterium tuberculosis isolates from MDR-TB risk patients in Thailand |
| title_fullStr | Characterization of katG, inhA, rpoB and pncA in Mycobacterium tuberculosis isolates from MDR-TB risk patients in Thailand |
| title_full_unstemmed | Characterization of katG, inhA, rpoB and pncA in Mycobacterium tuberculosis isolates from MDR-TB risk patients in Thailand |
| title_short | Characterization of katG, inhA, rpoB and pncA in Mycobacterium tuberculosis isolates from MDR-TB risk patients in Thailand |
| title_sort | characterization of katg inha rpob and pnca in mycobacterium tuberculosis isolates from mdr tb risk patients in thailand |
| topic | multidrug-resistant tuberculosis MDR-TB mutation DNA sequencing |
| url | https://jidc.org/index.php/journal/article/view/11974 |
| work_keys_str_mv | AT krairerksuthum characterizationofkatginharpobandpncainmycobacteriumtuberculosisisolatesfrommdrtbriskpatientsinthailand AT woradasamosornsuk characterizationofkatginharpobandpncainmycobacteriumtuberculosisisolatesfrommdrtbriskpatientsinthailand AT seksunsamosornsuk characterizationofkatginharpobandpncainmycobacteriumtuberculosisisolatesfrommdrtbriskpatientsinthailand |