Anthocyanin Extracts From 11 Superfruits Mitigate Oxidative Stress and Inflammatory Damage in HK‐2 Cells Induced by Cadmium Exposure
ABSTRACT Cadmium (Cd) pollution has emerged as an escalating threat to human health, with its induced kidney toxicity being closely linked to oxidative stress and inflammation. In this study, we extracted and analyzed primary anthocyanins from 11 superfruits using UPLC‐Triple‐TOF/MS and evaluated th...
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| Format: | Article |
| Language: | English |
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Wiley
2025-05-01
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| Series: | Food Frontiers |
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| Online Access: | https://doi.org/10.1002/fft2.70019 |
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| author | Jiaojiao Liang Yunyi Chen Manxi Wu Jiebiao Chen Cui Sun Jinping Cao Yue Wang Chongde Sun |
| author_facet | Jiaojiao Liang Yunyi Chen Manxi Wu Jiebiao Chen Cui Sun Jinping Cao Yue Wang Chongde Sun |
| author_sort | Jiaojiao Liang |
| collection | DOAJ |
| description | ABSTRACT Cadmium (Cd) pollution has emerged as an escalating threat to human health, with its induced kidney toxicity being closely linked to oxidative stress and inflammation. In this study, we extracted and analyzed primary anthocyanins from 11 superfruits using UPLC‐Triple‐TOF/MS and evaluated their antioxidant capacity through DPPH, ABTS, FRAP, and ORAC assays. By employing a Cd‐exposed Human Kidney‐2 (HK‐2) cells injury model, we investigated the protective effects of superfruit anthocyanin extracts. The results demonstrated that these extracts enhanced HK‐2 cell viability, increased T‐SOD and GSH levels, and reduced ROS, MDA, and NO. Furthermore, they significantly regulated oxidative stress and inflammation‐related genes and proteins. Moreover, we elucidated the mechanisms by which these anthocyanins mitigate Cd‐induced cellular damage via classical oxidative and inflammatory pathways. These findings highlight the potential of specific anthocyanin‐rich fruits in reducing Cd‐related health risks and suggest practical applications for their bioactive properties in promoting human health. |
| format | Article |
| id | doaj-art-04dafd6951bd41dabb2ff27e93bc58ea |
| institution | Kabale University |
| issn | 2643-8429 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Wiley |
| record_format | Article |
| series | Food Frontiers |
| spelling | doaj-art-04dafd6951bd41dabb2ff27e93bc58ea2025-08-20T03:48:22ZengWileyFood Frontiers2643-84292025-05-01631558157410.1002/fft2.70019Anthocyanin Extracts From 11 Superfruits Mitigate Oxidative Stress and Inflammatory Damage in HK‐2 Cells Induced by Cadmium ExposureJiaojiao Liang0Yunyi Chen1Manxi Wu2Jiebiao Chen3Cui Sun4Jinping Cao5Yue Wang6Chongde Sun7Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Fruit Science Institute, College of Agriculture and Biotechnology Zhejiang University Hangzhou ChinaLaboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Fruit Science Institute, College of Agriculture and Biotechnology Zhejiang University Hangzhou ChinaLaboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Fruit Science Institute, College of Agriculture and Biotechnology Zhejiang University Hangzhou ChinaLaboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Fruit Science Institute, College of Agriculture and Biotechnology Zhejiang University Hangzhou ChinaHainan Institute of Zhejiang University Sanya Hainan People's Republic of ChinaLaboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Fruit Science Institute, College of Agriculture and Biotechnology Zhejiang University Hangzhou ChinaLaboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Fruit Science Institute, College of Agriculture and Biotechnology Zhejiang University Hangzhou ChinaLaboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Fruit Science Institute, College of Agriculture and Biotechnology Zhejiang University Hangzhou ChinaABSTRACT Cadmium (Cd) pollution has emerged as an escalating threat to human health, with its induced kidney toxicity being closely linked to oxidative stress and inflammation. In this study, we extracted and analyzed primary anthocyanins from 11 superfruits using UPLC‐Triple‐TOF/MS and evaluated their antioxidant capacity through DPPH, ABTS, FRAP, and ORAC assays. By employing a Cd‐exposed Human Kidney‐2 (HK‐2) cells injury model, we investigated the protective effects of superfruit anthocyanin extracts. The results demonstrated that these extracts enhanced HK‐2 cell viability, increased T‐SOD and GSH levels, and reduced ROS, MDA, and NO. Furthermore, they significantly regulated oxidative stress and inflammation‐related genes and proteins. Moreover, we elucidated the mechanisms by which these anthocyanins mitigate Cd‐induced cellular damage via classical oxidative and inflammatory pathways. These findings highlight the potential of specific anthocyanin‐rich fruits in reducing Cd‐related health risks and suggest practical applications for their bioactive properties in promoting human health.https://doi.org/10.1002/fft2.70019anthocyanincadmiumoxidative stresssuperfruits |
| spellingShingle | Jiaojiao Liang Yunyi Chen Manxi Wu Jiebiao Chen Cui Sun Jinping Cao Yue Wang Chongde Sun Anthocyanin Extracts From 11 Superfruits Mitigate Oxidative Stress and Inflammatory Damage in HK‐2 Cells Induced by Cadmium Exposure Food Frontiers anthocyanin cadmium oxidative stress superfruits |
| title | Anthocyanin Extracts From 11 Superfruits Mitigate Oxidative Stress and Inflammatory Damage in HK‐2 Cells Induced by Cadmium Exposure |
| title_full | Anthocyanin Extracts From 11 Superfruits Mitigate Oxidative Stress and Inflammatory Damage in HK‐2 Cells Induced by Cadmium Exposure |
| title_fullStr | Anthocyanin Extracts From 11 Superfruits Mitigate Oxidative Stress and Inflammatory Damage in HK‐2 Cells Induced by Cadmium Exposure |
| title_full_unstemmed | Anthocyanin Extracts From 11 Superfruits Mitigate Oxidative Stress and Inflammatory Damage in HK‐2 Cells Induced by Cadmium Exposure |
| title_short | Anthocyanin Extracts From 11 Superfruits Mitigate Oxidative Stress and Inflammatory Damage in HK‐2 Cells Induced by Cadmium Exposure |
| title_sort | anthocyanin extracts from 11 superfruits mitigate oxidative stress and inflammatory damage in hk 2 cells induced by cadmium exposure |
| topic | anthocyanin cadmium oxidative stress superfruits |
| url | https://doi.org/10.1002/fft2.70019 |
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