Investigation on the Therapeutic Effect of Polygonum multiflorum Thunb. in Chronic Stress-Induced Hair Loss in Mice Coupled with Metabolomics and Proteomics
Objective: Stress is the main factor that leads to hair loss, and no effective therapeutics have been developed for stress alopecia. Polygonum multiflorum Thunb. (PM) is a famous traditional Chinese medicine for tonifying the kidney and nourishing the liver, which is used to treat hair loss. This st...
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| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2025-01-01
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| Series: | World Journal of Traditional Chinese Medicine |
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| Online Access: | https://journals.lww.com/10.4103/wjtcm.wjtcm_78_24 |
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| author | Yong-Kang Zhao Rui-Xian Guo Rui-Sheng Li Wei Shi Hai-Ying Gong Run-Ran Ma Han Gao Zhen Li Ke-Jian Hu Zhao-Fang Bai Xiao-He Xiao Qing-Ce Zang Wei-Jun Kong Peng Li Ming Niu Jia-Bo Wang Yuan Gao |
| author_facet | Yong-Kang Zhao Rui-Xian Guo Rui-Sheng Li Wei Shi Hai-Ying Gong Run-Ran Ma Han Gao Zhen Li Ke-Jian Hu Zhao-Fang Bai Xiao-He Xiao Qing-Ce Zang Wei-Jun Kong Peng Li Ming Niu Jia-Bo Wang Yuan Gao |
| author_sort | Yong-Kang Zhao |
| collection | DOAJ |
| description | Objective:
Stress is the main factor that leads to hair loss, and no effective therapeutics have been developed for stress alopecia. Polygonum multiflorum Thunb. (PM) is a famous traditional Chinese medicine for tonifying the kidney and nourishing the liver, which is used to treat hair loss. This study aimed to demonstrate that PM is a promising agent for the treatment of chronic stress-induced hair loss.
Materials and Methods:
C57BL/6J male mice were subjected to chronic restraint stress to inhibit hair follicle growth. Subsequently, the effect of PM was examined by oral administration of the PM water extract or cortisone, followed by histological analysis, immunofluorescence of follicle samples, serum metabolomics, and adrenal proteomics.
Results:
Hair regeneration in a mouse model was severely inhibited by chronic restraint stress. After PM treatment, hair regeneration in model mice was promoted, including an increased length of hair shafts in skin sections, an increased number of 5-Ethynyl-2’-deoxyuridine-positive cells, and a decreased number of apoptotic cells around the hair follicles. The various test indicators of these PM-treated mice almost recovered to levels comparable to those of the control mice. Through metabolomics and proteomics analyses, we identified 181 endogenous differential metabolites and 75 differential proteins, which may be the potential targets for PM. Pathway enrichment analysis showed that steroid biosynthesis (the main pathway through which stress leads to an imbalance in glucocorticoid secretion by the adrenal gland) may play an important role in the therapeutic effects of PM. In addition, omics analysis revealed that glycolysis/gluconeogenesis, pyruvate metabolism, lipid metabolism, and other glucocorticoid-related metabolic pathways differed significantly after PM treatment.
Conclusions:
PM is an important natural drug that regulates hair regeneration in mice under stress, and its underlying mechanism is closely related to the regulation of adrenal glucocorticoids and their metabolism. |
| format | Article |
| id | doaj-art-04bc8fa182a449688a918d8522152f52 |
| institution | DOAJ |
| issn | 2311-8571 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | World Journal of Traditional Chinese Medicine |
| spelling | doaj-art-04bc8fa182a449688a918d8522152f522025-08-20T02:57:44ZengWolters Kluwer Medknow PublicationsWorld Journal of Traditional Chinese Medicine2311-85712025-01-0111113414710.4103/wjtcm.wjtcm_78_24Investigation on the Therapeutic Effect of Polygonum multiflorum Thunb. in Chronic Stress-Induced Hair Loss in Mice Coupled with Metabolomics and ProteomicsYong-Kang ZhaoRui-Xian GuoRui-Sheng LiWei ShiHai-Ying GongRun-Ran MaHan GaoZhen LiKe-Jian HuZhao-Fang BaiXiao-He XiaoQing-Ce ZangWei-Jun KongPeng LiMing NiuJia-Bo WangYuan GaoObjective: Stress is the main factor that leads to hair loss, and no effective therapeutics have been developed for stress alopecia. Polygonum multiflorum Thunb. (PM) is a famous traditional Chinese medicine for tonifying the kidney and nourishing the liver, which is used to treat hair loss. This study aimed to demonstrate that PM is a promising agent for the treatment of chronic stress-induced hair loss. Materials and Methods: C57BL/6J male mice were subjected to chronic restraint stress to inhibit hair follicle growth. Subsequently, the effect of PM was examined by oral administration of the PM water extract or cortisone, followed by histological analysis, immunofluorescence of follicle samples, serum metabolomics, and adrenal proteomics. Results: Hair regeneration in a mouse model was severely inhibited by chronic restraint stress. After PM treatment, hair regeneration in model mice was promoted, including an increased length of hair shafts in skin sections, an increased number of 5-Ethynyl-2’-deoxyuridine-positive cells, and a decreased number of apoptotic cells around the hair follicles. The various test indicators of these PM-treated mice almost recovered to levels comparable to those of the control mice. Through metabolomics and proteomics analyses, we identified 181 endogenous differential metabolites and 75 differential proteins, which may be the potential targets for PM. Pathway enrichment analysis showed that steroid biosynthesis (the main pathway through which stress leads to an imbalance in glucocorticoid secretion by the adrenal gland) may play an important role in the therapeutic effects of PM. In addition, omics analysis revealed that glycolysis/gluconeogenesis, pyruvate metabolism, lipid metabolism, and other glucocorticoid-related metabolic pathways differed significantly after PM treatment. Conclusions: PM is an important natural drug that regulates hair regeneration in mice under stress, and its underlying mechanism is closely related to the regulation of adrenal glucocorticoids and their metabolism.https://journals.lww.com/10.4103/wjtcm.wjtcm_78_24chronic stressglucocorticoidshair regenerationpolygonum multiflorum thunb.traditional chinese medicine |
| spellingShingle | Yong-Kang Zhao Rui-Xian Guo Rui-Sheng Li Wei Shi Hai-Ying Gong Run-Ran Ma Han Gao Zhen Li Ke-Jian Hu Zhao-Fang Bai Xiao-He Xiao Qing-Ce Zang Wei-Jun Kong Peng Li Ming Niu Jia-Bo Wang Yuan Gao Investigation on the Therapeutic Effect of Polygonum multiflorum Thunb. in Chronic Stress-Induced Hair Loss in Mice Coupled with Metabolomics and Proteomics World Journal of Traditional Chinese Medicine chronic stress glucocorticoids hair regeneration polygonum multiflorum thunb. traditional chinese medicine |
| title | Investigation on the Therapeutic Effect of Polygonum multiflorum Thunb. in Chronic Stress-Induced Hair Loss in Mice Coupled with Metabolomics and Proteomics |
| title_full | Investigation on the Therapeutic Effect of Polygonum multiflorum Thunb. in Chronic Stress-Induced Hair Loss in Mice Coupled with Metabolomics and Proteomics |
| title_fullStr | Investigation on the Therapeutic Effect of Polygonum multiflorum Thunb. in Chronic Stress-Induced Hair Loss in Mice Coupled with Metabolomics and Proteomics |
| title_full_unstemmed | Investigation on the Therapeutic Effect of Polygonum multiflorum Thunb. in Chronic Stress-Induced Hair Loss in Mice Coupled with Metabolomics and Proteomics |
| title_short | Investigation on the Therapeutic Effect of Polygonum multiflorum Thunb. in Chronic Stress-Induced Hair Loss in Mice Coupled with Metabolomics and Proteomics |
| title_sort | investigation on the therapeutic effect of polygonum multiflorum thunb in chronic stress induced hair loss in mice coupled with metabolomics and proteomics |
| topic | chronic stress glucocorticoids hair regeneration polygonum multiflorum thunb. traditional chinese medicine |
| url | https://journals.lww.com/10.4103/wjtcm.wjtcm_78_24 |
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