Genome-wide identification and expression analysis of the PIN gene family in the pepper pedicel abscission zone and during root development
[Objective] This study seeks to identify and analyze the PIN gene family members in pepper (Capsicum annuum L.) and to investigate their expression patterns in the abscission zone of the fruit pedicel as well as during root development. The ultimate goal is to provide candidate genes for elucidati...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | zho |
| Published: |
Science Press
2024-12-01
|
| Series: | Xibei zhiwu xuebao |
| Subjects: | |
| Online Access: | http://xbzwxb.alljournal.net/xbzwxb/article/abstract/20240347?st=article_issue |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1823860547247931392 |
|---|---|
| author | HE Lei YAN Xi LAI Wei LIU Chongzheng YANG Hong HE Jianwen |
| author_facet | HE Lei YAN Xi LAI Wei LIU Chongzheng YANG Hong HE Jianwen |
| author_sort | HE Lei |
| collection | DOAJ |
| description | [Objective] This study seeks to identify and analyze the PIN gene family members in pepper
(Capsicum annuum L.) and to investigate their expression patterns in the abscission zone of the fruit pedicel
as well as during root development. The ultimate goal is to provide candidate genes for elucidating the
function of CaPINs and for breeding new pepper varieties. [Methods] PIN genes were identified through
whole-genome screening of pepper, followed by comprehensive bioinformatics analysis. The expression
patterns of these genes in the fruit pedicel abscission zone and during root development were systematically
analyzed. [Results] 9 members of the PIN gene family were identified in the pepper genome and designated
as CaPIN1 through CaPIN9. These genes are distributed across seven chromosomes, with CaPIN9
remaining unanchored. The proteins encoded by these genes exhibit amino acid length ranging from 358 to
654 residues, molecular weight from 39 251.05 D to 71 170.91 D, and isoelectric point from 6.40 to
9.38. Most of these proteins are stable. Additionally, significant collinearity was observed among the
CaPINs, indicating a close genetic relationship with the tomato genome. CaPINs encompass a multitude
of elements associated with growth, environmental responses, and hormonal signaling. Transcriptome data
analysis revealed that the expression of CaPINs in pepper was tissue-specific. Quantitative PCR results
demonstrated that CaPINs exhibited differential expression levels across various fruit pedicel abscission
zones and throughout root development. [Conclusion] CaPINs play a crucial role in the development of
the pedicel abscission zone and the growth and development of the root system. These genes hold significant
potential as candidates for breeding pepper varieties with improved ease of de-stemming and for enhancing
genetic traits related to root systems. |
| format | Article |
| id | doaj-art-04b7a9ed00b14df8a17484aad81c84a1 |
| institution | Kabale University |
| issn | 1000-4025 |
| language | zho |
| publishDate | 2024-12-01 |
| publisher | Science Press |
| record_format | Article |
| series | Xibei zhiwu xuebao |
| spelling | doaj-art-04b7a9ed00b14df8a17484aad81c84a12025-02-10T12:26:19ZzhoScience PressXibei zhiwu xuebao1000-40252024-12-0144121878188910.7606/j.issn.1000-4025.20240347Genome-wide identification and expression analysis of the PIN gene family in the pepper pedicel abscission zone and during root developmentHE Lei0YAN Xi1LAI Wei2LIU Chongzheng3YANG Hong4HE Jianwen5Institute of Pepper, Guizhou Academy of Agricultural Sciences, Guiyang 550025, ChinaInstitute of Pepper, Guizhou Academy of Agricultural Sciences, Guiyang 550025, ChinaInstitute of Pepper, Guizhou Academy of Agricultural Sciences, Guiyang 550025, ChinaInstitute of Pepper, Guizhou Academy of Agricultural Sciences, Guiyang 550025, ChinaInstitute of Pepper, Guizhou Academy of Agricultural Sciences, Guiyang 550025, ChinaInstitute of Pepper, Guizhou Academy of Agricultural Sciences, Guiyang 550025, China[Objective] This study seeks to identify and analyze the PIN gene family members in pepper (Capsicum annuum L.) and to investigate their expression patterns in the abscission zone of the fruit pedicel as well as during root development. The ultimate goal is to provide candidate genes for elucidating the function of CaPINs and for breeding new pepper varieties. [Methods] PIN genes were identified through whole-genome screening of pepper, followed by comprehensive bioinformatics analysis. The expression patterns of these genes in the fruit pedicel abscission zone and during root development were systematically analyzed. [Results] 9 members of the PIN gene family were identified in the pepper genome and designated as CaPIN1 through CaPIN9. These genes are distributed across seven chromosomes, with CaPIN9 remaining unanchored. The proteins encoded by these genes exhibit amino acid length ranging from 358 to 654 residues, molecular weight from 39 251.05 D to 71 170.91 D, and isoelectric point from 6.40 to 9.38. Most of these proteins are stable. Additionally, significant collinearity was observed among the CaPINs, indicating a close genetic relationship with the tomato genome. CaPINs encompass a multitude of elements associated with growth, environmental responses, and hormonal signaling. Transcriptome data analysis revealed that the expression of CaPINs in pepper was tissue-specific. Quantitative PCR results demonstrated that CaPINs exhibited differential expression levels across various fruit pedicel abscission zones and throughout root development. [Conclusion] CaPINs play a crucial role in the development of the pedicel abscission zone and the growth and development of the root system. These genes hold significant potential as candidates for breeding pepper varieties with improved ease of de-stemming and for enhancing genetic traits related to root systems.http://xbzwxb.alljournal.net/xbzwxb/article/abstract/20240347?st=article_issuepepperpedicelspin gene familybioinformaticsexpression analysis |
| spellingShingle | HE Lei YAN Xi LAI Wei LIU Chongzheng YANG Hong HE Jianwen Genome-wide identification and expression analysis of the PIN gene family in the pepper pedicel abscission zone and during root development Xibei zhiwu xuebao pepper pedicels pin gene family bioinformatics expression analysis |
| title | Genome-wide identification and expression analysis of the PIN gene family in the pepper pedicel abscission zone and during root development |
| title_full | Genome-wide identification and expression analysis of the PIN gene family in the pepper pedicel abscission zone and during root development |
| title_fullStr | Genome-wide identification and expression analysis of the PIN gene family in the pepper pedicel abscission zone and during root development |
| title_full_unstemmed | Genome-wide identification and expression analysis of the PIN gene family in the pepper pedicel abscission zone and during root development |
| title_short | Genome-wide identification and expression analysis of the PIN gene family in the pepper pedicel abscission zone and during root development |
| title_sort | genome wide identification and expression analysis of the pin gene family in the pepper pedicel abscission zone and during root development |
| topic | pepper pedicels pin gene family bioinformatics expression analysis |
| url | http://xbzwxb.alljournal.net/xbzwxb/article/abstract/20240347?st=article_issue |
| work_keys_str_mv | AT helei genomewideidentificationandexpressionanalysisofthepingenefamilyinthepepperpedicelabscissionzoneandduringrootdevelopment AT yanxi genomewideidentificationandexpressionanalysisofthepingenefamilyinthepepperpedicelabscissionzoneandduringrootdevelopment AT laiwei genomewideidentificationandexpressionanalysisofthepingenefamilyinthepepperpedicelabscissionzoneandduringrootdevelopment AT liuchongzheng genomewideidentificationandexpressionanalysisofthepingenefamilyinthepepperpedicelabscissionzoneandduringrootdevelopment AT yanghong genomewideidentificationandexpressionanalysisofthepingenefamilyinthepepperpedicelabscissionzoneandduringrootdevelopment AT hejianwen genomewideidentificationandexpressionanalysisofthepingenefamilyinthepepperpedicelabscissionzoneandduringrootdevelopment |