Genome-wide identification and expression analysis of the PIN gene family in the pepper pedicel abscission zone and during root development
[Objective] This study seeks to identify and analyze the PIN gene family members in pepper (Capsicum annuum L.) and to investigate their expression patterns in the abscission zone of the fruit pedicel as well as during root development. The ultimate goal is to provide candidate genes for elucidati...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | zho |
| Published: |
Science Press
2024-12-01
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| Series: | Xibei zhiwu xuebao |
| Subjects: | |
| Online Access: | http://xbzwxb.alljournal.net/xbzwxb/article/abstract/20240347?st=article_issue |
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| Summary: | [Objective] This study seeks to identify and analyze the PIN gene family members in pepper
(Capsicum annuum L.) and to investigate their expression patterns in the abscission zone of the fruit pedicel
as well as during root development. The ultimate goal is to provide candidate genes for elucidating the
function of CaPINs and for breeding new pepper varieties. [Methods] PIN genes were identified through
whole-genome screening of pepper, followed by comprehensive bioinformatics analysis. The expression
patterns of these genes in the fruit pedicel abscission zone and during root development were systematically
analyzed. [Results] 9 members of the PIN gene family were identified in the pepper genome and designated
as CaPIN1 through CaPIN9. These genes are distributed across seven chromosomes, with CaPIN9
remaining unanchored. The proteins encoded by these genes exhibit amino acid length ranging from 358 to
654 residues, molecular weight from 39 251.05 D to 71 170.91 D, and isoelectric point from 6.40 to
9.38. Most of these proteins are stable. Additionally, significant collinearity was observed among the
CaPINs, indicating a close genetic relationship with the tomato genome. CaPINs encompass a multitude
of elements associated with growth, environmental responses, and hormonal signaling. Transcriptome data
analysis revealed that the expression of CaPINs in pepper was tissue-specific. Quantitative PCR results
demonstrated that CaPINs exhibited differential expression levels across various fruit pedicel abscission
zones and throughout root development. [Conclusion] CaPINs play a crucial role in the development of
the pedicel abscission zone and the growth and development of the root system. These genes hold significant
potential as candidates for breeding pepper varieties with improved ease of de-stemming and for enhancing
genetic traits related to root systems. |
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| ISSN: | 1000-4025 |