Inoculation and co-inoculation of lettuce and arugula hydroponically influence nitrogen metabolism, plant growth, nutrient acquisition and photosynthesis

The objective of this study was to investigate the effects of single and combined inoculations of A. brasilense, B. subtilis and P. fluorescens on lettuce and arugula grown in a hydroponic system. The study was carried out in a greenhouse and was designed in randomized blocks with five replications....

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Main Authors: Carlos Eduardo da Silva Oliveira, Thaissa Julyanne Soares Sena Oliveira, Arshad Jalal, Guilherme Carlos Fernandes, Andréa de Castro Bastos, Marcelo Rinaldi da Silva, Gabriela Rodrigues Sant’Ana, Jailson Vieira Aguilar, Liliane Santos de Camargos, Tiago Zoz, Marcelo Carvalho Minhoto Teixeira Filho
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-04-01
Series:Frontiers in Plant Science
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Online Access:https://www.frontiersin.org/articles/10.3389/fpls.2025.1547821/full
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author Carlos Eduardo da Silva Oliveira
Thaissa Julyanne Soares Sena Oliveira
Arshad Jalal
Guilherme Carlos Fernandes
Andréa de Castro Bastos
Andréa de Castro Bastos
Marcelo Rinaldi da Silva
Gabriela Rodrigues Sant’Ana
Jailson Vieira Aguilar
Liliane Santos de Camargos
Tiago Zoz
Marcelo Carvalho Minhoto Teixeira Filho
author_facet Carlos Eduardo da Silva Oliveira
Thaissa Julyanne Soares Sena Oliveira
Arshad Jalal
Guilherme Carlos Fernandes
Andréa de Castro Bastos
Andréa de Castro Bastos
Marcelo Rinaldi da Silva
Gabriela Rodrigues Sant’Ana
Jailson Vieira Aguilar
Liliane Santos de Camargos
Tiago Zoz
Marcelo Carvalho Minhoto Teixeira Filho
author_sort Carlos Eduardo da Silva Oliveira
collection DOAJ
description The objective of this study was to investigate the effects of single and combined inoculations of A. brasilense, B. subtilis and P. fluorescens on lettuce and arugula grown in a hydroponic system. The study was carried out in a greenhouse and was designed in randomized blocks with five replications. The treatments consisted of inoculation with A. brasilense, B. subtilis and P. fluorescens and co-inoculation with A. brasilense + B. subtilis, A. brasilense + P. fluorescens, B. subtilis + P. fluorescens and A. brasilense + B. subtilis + P. fluorescens via nutrient solution. An increase in the length and fresh mass of the shoot and leaf chlorophyll concentrations of arugula and lettuce was observed under co-inoculations of A. brasilense + P. fluorescens and B. subtilis + P. fluorescens. Greater length, fresh mass and volume of the lettuce root system were observed under the co-inoculations of A. brasilense + B. subtilis, A. brasilense + P. fluorescens and B. subtilis + P. fluorescens in arugula under the inoculations of A. brasilense and A. brasilense + P. fluorescens. Greater nitrate reductase activity was detected in leaves, and lower nitrate accumulation was detected in lettuce and arugula under inoculations with A. brasilense, P. fluorescens and B. subtilis + P. fluorescens. The greatest accumulation of N, P, K, Ca and Mg in the lettuce shoot was obtained under inoculation with P. fluorescens, A. brasilense + P. fluorescens and B. subtilis + P. fluorescens. Co-inoculation with A. brasilense + P. fluorescens and B. subtilis + P. fluorescens was the most efficient combination for increasing the growth, nutrient acquisition and functioning of nitrogen metabolism in arugula lettuce plants.
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spelling doaj-art-04b08b97b4564ad080f4f18d9159eeea2025-08-20T02:11:54ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-04-011610.3389/fpls.2025.15478211547821Inoculation and co-inoculation of lettuce and arugula hydroponically influence nitrogen metabolism, plant growth, nutrient acquisition and photosynthesisCarlos Eduardo da Silva Oliveira0Thaissa Julyanne Soares Sena Oliveira1Arshad Jalal2Guilherme Carlos Fernandes3Andréa de Castro Bastos4Andréa de Castro Bastos5Marcelo Rinaldi da Silva6Gabriela Rodrigues Sant’Ana7Jailson Vieira Aguilar8Liliane Santos de Camargos9Tiago Zoz10Marcelo Carvalho Minhoto Teixeira Filho11Department of Agronomy, State University of Mato Grosso do Sul – UEMS, Cassilândia, Mato Grosso do Sul, BrazilDepartment of Plant Protection, Rural Engineering and Soils, São Paulo State University (UNESP), School of Engineering, Ilha Solteira, São Paulo, BrazilThe BioActives Lab, Center for Desert Agriculture (CDA) Division of Biological and Environmental Sciences (BESE) King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi ArabiaDepartment of Plant Protection, Rural Engineering and Soils, São Paulo State University (UNESP), School of Engineering, Ilha Solteira, São Paulo, BrazilDepartment of Plant Protection, Rural Engineering and Soils, São Paulo State University (UNESP), School of Engineering, Ilha Solteira, São Paulo, BrazilSalesian Auxilium Catholic University Center, Araçatuba, São Paulo, BrazilDepartment of Plant Protection, Rural Engineering and Soils, São Paulo State University (UNESP), School of Engineering, Ilha Solteira, São Paulo, BrazilDepartment of Agronomy, State University of Mato Grosso do Sul – UEMS, Cassilândia, Mato Grosso do Sul, BrazilDepartment of Biology and Zootechny, São Paulo State University (UNESP), School of Engineering, Ilha Solteira, São Paulo, BrazilDepartment of Biology and Zootechny, São Paulo State University (UNESP), School of Engineering, Ilha Solteira, São Paulo, BrazilDepartment of Agronomy, State University of Mato Grosso do Sul – UEMS, Mundo Novol, Mato Grosso do Sul, BrazilDepartment of Plant Protection, Rural Engineering and Soils, São Paulo State University (UNESP), School of Engineering, Ilha Solteira, São Paulo, BrazilThe objective of this study was to investigate the effects of single and combined inoculations of A. brasilense, B. subtilis and P. fluorescens on lettuce and arugula grown in a hydroponic system. The study was carried out in a greenhouse and was designed in randomized blocks with five replications. The treatments consisted of inoculation with A. brasilense, B. subtilis and P. fluorescens and co-inoculation with A. brasilense + B. subtilis, A. brasilense + P. fluorescens, B. subtilis + P. fluorescens and A. brasilense + B. subtilis + P. fluorescens via nutrient solution. An increase in the length and fresh mass of the shoot and leaf chlorophyll concentrations of arugula and lettuce was observed under co-inoculations of A. brasilense + P. fluorescens and B. subtilis + P. fluorescens. Greater length, fresh mass and volume of the lettuce root system were observed under the co-inoculations of A. brasilense + B. subtilis, A. brasilense + P. fluorescens and B. subtilis + P. fluorescens in arugula under the inoculations of A. brasilense and A. brasilense + P. fluorescens. Greater nitrate reductase activity was detected in leaves, and lower nitrate accumulation was detected in lettuce and arugula under inoculations with A. brasilense, P. fluorescens and B. subtilis + P. fluorescens. The greatest accumulation of N, P, K, Ca and Mg in the lettuce shoot was obtained under inoculation with P. fluorescens, A. brasilense + P. fluorescens and B. subtilis + P. fluorescens. Co-inoculation with A. brasilense + P. fluorescens and B. subtilis + P. fluorescens was the most efficient combination for increasing the growth, nutrient acquisition and functioning of nitrogen metabolism in arugula lettuce plants.https://www.frontiersin.org/articles/10.3389/fpls.2025.1547821/fullamino acid contentcarbohydrate contentcarbon assimilationchlorophyllnitrate reductaseplant growth-promoting bacteria
spellingShingle Carlos Eduardo da Silva Oliveira
Thaissa Julyanne Soares Sena Oliveira
Arshad Jalal
Guilherme Carlos Fernandes
Andréa de Castro Bastos
Andréa de Castro Bastos
Marcelo Rinaldi da Silva
Gabriela Rodrigues Sant’Ana
Jailson Vieira Aguilar
Liliane Santos de Camargos
Tiago Zoz
Marcelo Carvalho Minhoto Teixeira Filho
Inoculation and co-inoculation of lettuce and arugula hydroponically influence nitrogen metabolism, plant growth, nutrient acquisition and photosynthesis
Frontiers in Plant Science
amino acid content
carbohydrate content
carbon assimilation
chlorophyll
nitrate reductase
plant growth-promoting bacteria
title Inoculation and co-inoculation of lettuce and arugula hydroponically influence nitrogen metabolism, plant growth, nutrient acquisition and photosynthesis
title_full Inoculation and co-inoculation of lettuce and arugula hydroponically influence nitrogen metabolism, plant growth, nutrient acquisition and photosynthesis
title_fullStr Inoculation and co-inoculation of lettuce and arugula hydroponically influence nitrogen metabolism, plant growth, nutrient acquisition and photosynthesis
title_full_unstemmed Inoculation and co-inoculation of lettuce and arugula hydroponically influence nitrogen metabolism, plant growth, nutrient acquisition and photosynthesis
title_short Inoculation and co-inoculation of lettuce and arugula hydroponically influence nitrogen metabolism, plant growth, nutrient acquisition and photosynthesis
title_sort inoculation and co inoculation of lettuce and arugula hydroponically influence nitrogen metabolism plant growth nutrient acquisition and photosynthesis
topic amino acid content
carbohydrate content
carbon assimilation
chlorophyll
nitrate reductase
plant growth-promoting bacteria
url https://www.frontiersin.org/articles/10.3389/fpls.2025.1547821/full
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