Molecular Characterization of Bovine Deltapapillomavirus in Equine Sarcoids in Egypt
Bovine papillomaviruses (BPVs) commonly cause sarcoids in equines worldwide. Equine sarcoids (ESs) reduce the working ability of draft animals and produce untoward cosmetic changes in racing and dancing equine. In this study, nine horses and 16 donkeys with sarcoids were presented to Zagazig Univers...
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Wiley
2025-01-01
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| Series: | Veterinary Medicine International |
| Online Access: | http://dx.doi.org/10.1155/vmi/9773642 |
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| author | Nader Maher Sobhy Walid Refaai Rahul Kumar Christiana Rezk Bottros Youssef Sagar Mal Goyal |
| author_facet | Nader Maher Sobhy Walid Refaai Rahul Kumar Christiana Rezk Bottros Youssef Sagar Mal Goyal |
| author_sort | Nader Maher Sobhy |
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| description | Bovine papillomaviruses (BPVs) commonly cause sarcoids in equines worldwide. Equine sarcoids (ESs) reduce the working ability of draft animals and produce untoward cosmetic changes in racing and dancing equine. In this study, nine horses and 16 donkeys with sarcoids were presented to Zagazig University Veterinary Clinic, Zagazig, Egypt. Of these, eight horses and six donkeys were found to be infected with BPV. On sequencing, all 14 viruses were found to be BPV1, which were distributed in two clades without specific differentiation among papillomaviruses (PVs) of donkeys, horses, and cattle. Comparison of 135 aa (319–454) of the sequenced L1 gene with reference strains revealed three conservative mutations (D346N, Q398E, and F441Y) and two nonconservative mutations (T348N and K351T). Illumina sequencing revealed that PVs of donkeys and horses were identical and had 98.5% identity with the closest reference sequence (KX907623) of BPV1. In addition, there was high identity among all genes except E5 and L2. The substitution ranged between 0.5% (nt) and 0.89% (aa) in E4 and 5.18% (nt) and 6.81% (aa) in E5. These results indicate that BPV1 is the main cause of ESs in Egypt without marked phylogenetic variation among PVs of cattle, horses, and donkeys. |
| format | Article |
| id | doaj-art-04a61d7798594fdbb7aedfc4fdb20af6 |
| institution | DOAJ |
| issn | 2042-0048 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
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| series | Veterinary Medicine International |
| spelling | doaj-art-04a61d7798594fdbb7aedfc4fdb20af62025-08-20T03:12:35ZengWileyVeterinary Medicine International2042-00482025-01-01202510.1155/vmi/9773642Molecular Characterization of Bovine Deltapapillomavirus in Equine Sarcoids in EgyptNader Maher Sobhy0Walid Refaai1Rahul Kumar2Christiana Rezk Bottros Youssef3Sagar Mal Goyal4Department of Animal MedicineDepartment of Surgery, Anesthesiology, and RadiologyTennessee Department of AgricultureMicrobiology and Immunology DepartmentVeterinary Population Medicine Department and Veterinary Diagnostic LaboratoryBovine papillomaviruses (BPVs) commonly cause sarcoids in equines worldwide. Equine sarcoids (ESs) reduce the working ability of draft animals and produce untoward cosmetic changes in racing and dancing equine. In this study, nine horses and 16 donkeys with sarcoids were presented to Zagazig University Veterinary Clinic, Zagazig, Egypt. Of these, eight horses and six donkeys were found to be infected with BPV. On sequencing, all 14 viruses were found to be BPV1, which were distributed in two clades without specific differentiation among papillomaviruses (PVs) of donkeys, horses, and cattle. Comparison of 135 aa (319–454) of the sequenced L1 gene with reference strains revealed three conservative mutations (D346N, Q398E, and F441Y) and two nonconservative mutations (T348N and K351T). Illumina sequencing revealed that PVs of donkeys and horses were identical and had 98.5% identity with the closest reference sequence (KX907623) of BPV1. In addition, there was high identity among all genes except E5 and L2. The substitution ranged between 0.5% (nt) and 0.89% (aa) in E4 and 5.18% (nt) and 6.81% (aa) in E5. These results indicate that BPV1 is the main cause of ESs in Egypt without marked phylogenetic variation among PVs of cattle, horses, and donkeys.http://dx.doi.org/10.1155/vmi/9773642 |
| spellingShingle | Nader Maher Sobhy Walid Refaai Rahul Kumar Christiana Rezk Bottros Youssef Sagar Mal Goyal Molecular Characterization of Bovine Deltapapillomavirus in Equine Sarcoids in Egypt Veterinary Medicine International |
| title | Molecular Characterization of Bovine Deltapapillomavirus in Equine Sarcoids in Egypt |
| title_full | Molecular Characterization of Bovine Deltapapillomavirus in Equine Sarcoids in Egypt |
| title_fullStr | Molecular Characterization of Bovine Deltapapillomavirus in Equine Sarcoids in Egypt |
| title_full_unstemmed | Molecular Characterization of Bovine Deltapapillomavirus in Equine Sarcoids in Egypt |
| title_short | Molecular Characterization of Bovine Deltapapillomavirus in Equine Sarcoids in Egypt |
| title_sort | molecular characterization of bovine deltapapillomavirus in equine sarcoids in egypt |
| url | http://dx.doi.org/10.1155/vmi/9773642 |
| work_keys_str_mv | AT nadermahersobhy molecularcharacterizationofbovinedeltapapillomavirusinequinesarcoidsinegypt AT walidrefaai molecularcharacterizationofbovinedeltapapillomavirusinequinesarcoidsinegypt AT rahulkumar molecularcharacterizationofbovinedeltapapillomavirusinequinesarcoidsinegypt AT christianarezkbottrosyoussef molecularcharacterizationofbovinedeltapapillomavirusinequinesarcoidsinegypt AT sagarmalgoyal molecularcharacterizationofbovinedeltapapillomavirusinequinesarcoidsinegypt |