The developmental lipidome of Nippostrongylus brasiliensis
Abstract Background Nippostrongylus brasiliensis—a nematode of rodents—is commonly used as a model to study the immunobiology of parasitic nematodes. It is a member of the Strongylida—a large order of socioeconomically important parasitic nematodes of animals. Lipids are known to play essential role...
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BMC
2025-01-01
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| Series: | Parasites & Vectors |
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| Online Access: | https://doi.org/10.1186/s13071-024-06654-2 |
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| author | Tao Wang Michael G. Leeming Nicholas A. Williamson Tiffany Bouchery Rory Doolan Graham Le Gros Gavin E. Reid Nicola L. Harris Robin B. Gasser |
| author_facet | Tao Wang Michael G. Leeming Nicholas A. Williamson Tiffany Bouchery Rory Doolan Graham Le Gros Gavin E. Reid Nicola L. Harris Robin B. Gasser |
| author_sort | Tao Wang |
| collection | DOAJ |
| description | Abstract Background Nippostrongylus brasiliensis—a nematode of rodents—is commonly used as a model to study the immunobiology of parasitic nematodes. It is a member of the Strongylida—a large order of socioeconomically important parasitic nematodes of animals. Lipids are known to play essential roles in nematode biology, influencing cellular membranes, energy storage and/or signalling. Methods The present investigation provides a comprehensive, untargeted lipidomic analysis of four developmental stages/sexes (i.e. egg, L3, adult female and adult male stages) of N. brasiliensis utilising liquid chromatography coupled to mass spectrometry. Results We identified 464 lipid species representing 18 lipid classes and revealed distinct stage-specific changes in lipid composition throughout nematode development. Triacylglycerols (TGs) dominated the lipid profile in the egg stage, suggesting a key role for them in energy storage at this early developmental stage. As N. brasiliensis develops, there was a conspicuous transition toward membrane-associated lipids, including glycerophospholipids (e.g. PE and PC) and ether-linked lipids, particularly in adult stages, indicating a shift toward host adaptation and membrane stabilisation. Conclusions We provide a comprehensive insight into the lipid composition and abundance of key free-living and parasitic stages of N. brasiliensis. This study provides lipidomic resources to underpin the detailed exploration of lipid biology in this model parasitic nematode. Graphical Abstract |
| format | Article |
| id | doaj-art-04a2a664bf4d4bb881d0d415c022fc19 |
| institution | Kabale University |
| issn | 1756-3305 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
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| series | Parasites & Vectors |
| spelling | doaj-art-04a2a664bf4d4bb881d0d415c022fc192025-01-26T12:17:41ZengBMCParasites & Vectors1756-33052025-01-0118111010.1186/s13071-024-06654-2The developmental lipidome of Nippostrongylus brasiliensisTao Wang0Michael G. Leeming1Nicholas A. Williamson2Tiffany Bouchery3Rory Doolan4Graham Le Gros5Gavin E. Reid6Nicola L. Harris7Robin B. Gasser8Department of Veterinary Biosciences, Melbourne Veterinary School, Faculty of Science, The University of MelbourneBio21 Mass Spectrometry and Proteomics Facility, The University of MelbourneBio21 Mass Spectrometry and Proteomics Facility, The University of MelbourneDepartment of Medical Parasitology and Infection Biology, Swiss Tropical and Public Health InstituteDepartment of Medical Parasitology and Infection Biology, Swiss Tropical and Public Health InstituteMalaghan Institute of Medical ResearchSchool of Chemistry, The University of MelbourneDepartment of Immunology, School of Translational Medicine, Monash UniversityDepartment of Veterinary Biosciences, Melbourne Veterinary School, Faculty of Science, The University of MelbourneAbstract Background Nippostrongylus brasiliensis—a nematode of rodents—is commonly used as a model to study the immunobiology of parasitic nematodes. It is a member of the Strongylida—a large order of socioeconomically important parasitic nematodes of animals. Lipids are known to play essential roles in nematode biology, influencing cellular membranes, energy storage and/or signalling. Methods The present investigation provides a comprehensive, untargeted lipidomic analysis of four developmental stages/sexes (i.e. egg, L3, adult female and adult male stages) of N. brasiliensis utilising liquid chromatography coupled to mass spectrometry. Results We identified 464 lipid species representing 18 lipid classes and revealed distinct stage-specific changes in lipid composition throughout nematode development. Triacylglycerols (TGs) dominated the lipid profile in the egg stage, suggesting a key role for them in energy storage at this early developmental stage. As N. brasiliensis develops, there was a conspicuous transition toward membrane-associated lipids, including glycerophospholipids (e.g. PE and PC) and ether-linked lipids, particularly in adult stages, indicating a shift toward host adaptation and membrane stabilisation. Conclusions We provide a comprehensive insight into the lipid composition and abundance of key free-living and parasitic stages of N. brasiliensis. This study provides lipidomic resources to underpin the detailed exploration of lipid biology in this model parasitic nematode. Graphical Abstracthttps://doi.org/10.1186/s13071-024-06654-2Nippostrongylus brasiliensisGastrointestinal nematodeRodentLipidomeLipidsMass spectrometry |
| spellingShingle | Tao Wang Michael G. Leeming Nicholas A. Williamson Tiffany Bouchery Rory Doolan Graham Le Gros Gavin E. Reid Nicola L. Harris Robin B. Gasser The developmental lipidome of Nippostrongylus brasiliensis Parasites & Vectors Nippostrongylus brasiliensis Gastrointestinal nematode Rodent Lipidome Lipids Mass spectrometry |
| title | The developmental lipidome of Nippostrongylus brasiliensis |
| title_full | The developmental lipidome of Nippostrongylus brasiliensis |
| title_fullStr | The developmental lipidome of Nippostrongylus brasiliensis |
| title_full_unstemmed | The developmental lipidome of Nippostrongylus brasiliensis |
| title_short | The developmental lipidome of Nippostrongylus brasiliensis |
| title_sort | developmental lipidome of nippostrongylus brasiliensis |
| topic | Nippostrongylus brasiliensis Gastrointestinal nematode Rodent Lipidome Lipids Mass spectrometry |
| url | https://doi.org/10.1186/s13071-024-06654-2 |
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