Analysing the dhaT gene in Colombian Clostridium sp. (Clostridia) 1,3-propanediol-producing strains
To analyze the dhaT gene, one of the genes responsible for the 1,3-propanediol (1,3-PD) production, in two native Clostridiumstrains. Materials and methods: The dhaT gene was amplified by Polimerase Chain Reaction with specific primers designed fromClostridium butyricum VPI1718 operon. Bioinformatic...
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| Language: | English |
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Pontificia Universidad Javeriana
2010-04-01
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| Series: | Universitas Scientiarum |
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| Online Access: | http://revistas.javeriana.edu.co/index.php/scientarium/article/view/1375/838 |
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| author | Diana Milena Quilaguy-Ayure Dolly Montoya-Castaño José David Montoya-Solano Zulma Rocío Súarez-Moreno José Mauricio Bernal-Morales |
| author_facet | Diana Milena Quilaguy-Ayure Dolly Montoya-Castaño José David Montoya-Solano Zulma Rocío Súarez-Moreno José Mauricio Bernal-Morales |
| author_sort | Diana Milena Quilaguy-Ayure |
| collection | DOAJ |
| description | To analyze the dhaT gene, one of the genes responsible for the 1,3-propanediol (1,3-PD) production, in two native Clostridiumstrains. Materials and methods: The dhaT gene was amplified by Polimerase Chain Reaction with specific primers designed fromClostridium butyricum VPI1718 operon. Bioinformatics tools like BLASTN, ORF finder, BLASTP and ClustalW were used to determinethe identity of the sequence and to assign a function. Results: DNA amplification products were obtained from Colombian Clostridium sp.native strains (IBUN 13A and IBUN 158B) and the Clostridium butyricum DSM 2478 strain, which were sequenced. According to thebioinformatics analysis of the above sequences, a high degree of similarity was found with the dhaT gene of different bacterial species. Thehighest percentage of identity was obtained with the Clostridium butyricum VPI 1718 strain. Conclusion: knowledge of the physicalstructure of the 1,3-PD operon in native strains opens the way for developing genetic and metabolic engineering strategies for improvingprocesses productivity. |
| format | Article |
| id | doaj-art-04920a9096e54c11989f4eaed5d8820e |
| institution | OA Journals |
| issn | 0122-7483 2027-1352 |
| language | English |
| publishDate | 2010-04-01 |
| publisher | Pontificia Universidad Javeriana |
| record_format | Article |
| series | Universitas Scientiarum |
| spelling | doaj-art-04920a9096e54c11989f4eaed5d8820e2025-08-20T01:51:27ZengPontificia Universidad JaverianaUniversitas Scientiarum0122-74832027-13522010-04-011511726Analysing the dhaT gene in Colombian Clostridium sp. (Clostridia) 1,3-propanediol-producing strainsDiana Milena Quilaguy-AyureDolly Montoya-CastañoJosé David Montoya-SolanoZulma Rocío Súarez-MorenoJosé Mauricio Bernal-MoralesTo analyze the dhaT gene, one of the genes responsible for the 1,3-propanediol (1,3-PD) production, in two native Clostridiumstrains. Materials and methods: The dhaT gene was amplified by Polimerase Chain Reaction with specific primers designed fromClostridium butyricum VPI1718 operon. Bioinformatics tools like BLASTN, ORF finder, BLASTP and ClustalW were used to determinethe identity of the sequence and to assign a function. Results: DNA amplification products were obtained from Colombian Clostridium sp.native strains (IBUN 13A and IBUN 158B) and the Clostridium butyricum DSM 2478 strain, which were sequenced. According to thebioinformatics analysis of the above sequences, a high degree of similarity was found with the dhaT gene of different bacterial species. Thehighest percentage of identity was obtained with the Clostridium butyricum VPI 1718 strain. Conclusion: knowledge of the physicalstructure of the 1,3-PD operon in native strains opens the way for developing genetic and metabolic engineering strategies for improvingprocesses productivity.http://revistas.javeriana.edu.co/index.php/scientarium/article/view/1375/83813-propanediol13-propanediol dehydrogenasedhaT gene13-propanediol operon |
| spellingShingle | Diana Milena Quilaguy-Ayure Dolly Montoya-Castaño José David Montoya-Solano Zulma Rocío Súarez-Moreno José Mauricio Bernal-Morales Analysing the dhaT gene in Colombian Clostridium sp. (Clostridia) 1,3-propanediol-producing strains Universitas Scientiarum 1 3-propanediol 1 3-propanediol dehydrogenase dhaT gene 1 3-propanediol operon |
| title | Analysing the dhaT gene in Colombian Clostridium sp. (Clostridia) 1,3-propanediol-producing strains |
| title_full | Analysing the dhaT gene in Colombian Clostridium sp. (Clostridia) 1,3-propanediol-producing strains |
| title_fullStr | Analysing the dhaT gene in Colombian Clostridium sp. (Clostridia) 1,3-propanediol-producing strains |
| title_full_unstemmed | Analysing the dhaT gene in Colombian Clostridium sp. (Clostridia) 1,3-propanediol-producing strains |
| title_short | Analysing the dhaT gene in Colombian Clostridium sp. (Clostridia) 1,3-propanediol-producing strains |
| title_sort | analysing the dhat gene in colombian clostridium sp clostridia 1 3 propanediol producing strains |
| topic | 1 3-propanediol 1 3-propanediol dehydrogenase dhaT gene 1 3-propanediol operon |
| url | http://revistas.javeriana.edu.co/index.php/scientarium/article/view/1375/838 |
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