Molecular Mechanism of the <i>Grid</i> Gene Family Regulating Growth Size Heteromorphism in <i>Cynoglossus semilaevis</i>
There are obvious individual differences in the growth and development of <i>Cynoglossus semilaevis</i>, mainly due to female bias. We selected 500 female <i>Cynoglossus semilaevis</i> of different sizes for GWAS and transcriptome analysis to screen for differential genes. qP...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-04-01
|
| Series: | Animals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2615/15/8/1130 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850144597313847296 |
|---|---|
| author | Yaning Wang Yadong Chen Yang Liu Songlin Chen |
| author_facet | Yaning Wang Yadong Chen Yang Liu Songlin Chen |
| author_sort | Yaning Wang |
| collection | DOAJ |
| description | There are obvious individual differences in the growth and development of <i>Cynoglossus semilaevis</i>, mainly due to female bias. We selected 500 female <i>Cynoglossus semilaevis</i> of different sizes for GWAS and transcriptome analysis to screen for differential genes. qPCR was performed to detect the expression of the genes in various tissues, and RNAi experiments were performed in testicular cells to knock down the <i>grid1</i> and <i>grid2</i> genes and transcriptome sequencing was performed to check the changes of the downstream genes. <i>Grid</i> gene was screened for the common genes by GWAS sequencing and transcriptome sequencing. In the QPCR results, the expression of the <i>grid</i> gene family was negatively correlated with fish size, and was slightly higher in males than in females; in the transcriptome results, the expression of <i>shcbp1</i>, <i>sass6</i>, <i>cdca7</i>, and <i>gh</i> was up-regulated, and the expression of <i>igf1</i> was down-regulated. It is speculated that <i>igf1</i> has an antagonistic effect on <i>gh</i>, which is deregulated when the <i>grid</i> gene family is knocked down. The <i>grid</i> gene family may affect the growth of individual <i>Cynoglossus semilaevis</i> through the gh-igf1 axis, which provides a basis for the study of the differences in the growth size of <i>Cynoglossus semilaevis.</i> |
| format | Article |
| id | doaj-art-048dfbfe2afe459f8070b672ea7bb8cd |
| institution | OA Journals |
| issn | 2076-2615 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj-art-048dfbfe2afe459f8070b672ea7bb8cd2025-08-20T02:28:19ZengMDPI AGAnimals2076-26152025-04-01158113010.3390/ani15081130Molecular Mechanism of the <i>Grid</i> Gene Family Regulating Growth Size Heteromorphism in <i>Cynoglossus semilaevis</i>Yaning Wang0Yadong Chen1Yang Liu2Songlin Chen3College of Life Science, Qingdao University, Qingdao 266071, ChinaState Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, ChinaState Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, ChinaState Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, ChinaThere are obvious individual differences in the growth and development of <i>Cynoglossus semilaevis</i>, mainly due to female bias. We selected 500 female <i>Cynoglossus semilaevis</i> of different sizes for GWAS and transcriptome analysis to screen for differential genes. qPCR was performed to detect the expression of the genes in various tissues, and RNAi experiments were performed in testicular cells to knock down the <i>grid1</i> and <i>grid2</i> genes and transcriptome sequencing was performed to check the changes of the downstream genes. <i>Grid</i> gene was screened for the common genes by GWAS sequencing and transcriptome sequencing. In the QPCR results, the expression of the <i>grid</i> gene family was negatively correlated with fish size, and was slightly higher in males than in females; in the transcriptome results, the expression of <i>shcbp1</i>, <i>sass6</i>, <i>cdca7</i>, and <i>gh</i> was up-regulated, and the expression of <i>igf1</i> was down-regulated. It is speculated that <i>igf1</i> has an antagonistic effect on <i>gh</i>, which is deregulated when the <i>grid</i> gene family is knocked down. The <i>grid</i> gene family may affect the growth of individual <i>Cynoglossus semilaevis</i> through the gh-igf1 axis, which provides a basis for the study of the differences in the growth size of <i>Cynoglossus semilaevis.</i>https://www.mdpi.com/2076-2615/15/8/1130GWAStranscriptomegh-igf1 growth axis<i>cynoglossus semilaevis</i> |
| spellingShingle | Yaning Wang Yadong Chen Yang Liu Songlin Chen Molecular Mechanism of the <i>Grid</i> Gene Family Regulating Growth Size Heteromorphism in <i>Cynoglossus semilaevis</i> Animals GWAS transcriptome gh-igf1 growth axis <i>cynoglossus semilaevis</i> |
| title | Molecular Mechanism of the <i>Grid</i> Gene Family Regulating Growth Size Heteromorphism in <i>Cynoglossus semilaevis</i> |
| title_full | Molecular Mechanism of the <i>Grid</i> Gene Family Regulating Growth Size Heteromorphism in <i>Cynoglossus semilaevis</i> |
| title_fullStr | Molecular Mechanism of the <i>Grid</i> Gene Family Regulating Growth Size Heteromorphism in <i>Cynoglossus semilaevis</i> |
| title_full_unstemmed | Molecular Mechanism of the <i>Grid</i> Gene Family Regulating Growth Size Heteromorphism in <i>Cynoglossus semilaevis</i> |
| title_short | Molecular Mechanism of the <i>Grid</i> Gene Family Regulating Growth Size Heteromorphism in <i>Cynoglossus semilaevis</i> |
| title_sort | molecular mechanism of the i grid i gene family regulating growth size heteromorphism in i cynoglossus semilaevis i |
| topic | GWAS transcriptome gh-igf1 growth axis <i>cynoglossus semilaevis</i> |
| url | https://www.mdpi.com/2076-2615/15/8/1130 |
| work_keys_str_mv | AT yaningwang molecularmechanismoftheigridigenefamilyregulatinggrowthsizeheteromorphisminicynoglossussemilaevisi AT yadongchen molecularmechanismoftheigridigenefamilyregulatinggrowthsizeheteromorphisminicynoglossussemilaevisi AT yangliu molecularmechanismoftheigridigenefamilyregulatinggrowthsizeheteromorphisminicynoglossussemilaevisi AT songlinchen molecularmechanismoftheigridigenefamilyregulatinggrowthsizeheteromorphisminicynoglossussemilaevisi |