Complete mitochondrial genomes of Culicoides brevitarsis and Culicoides imicola biting midge vectors of Bluetongue Virus
Biting midges (Culicoides spp.) are important vectors of several insect borne arboviruses but are underrepresented in terms of availability of high-resolution genomic resources. We assembled and annotated complete mitochondrial genomes for two Culicoides species, namely C. brevitarsis and C. imicola...
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Taylor & Francis Group
2025-01-01
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| Series: | Mitochondrial DNA. Part B. Resources |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/23802359.2024.2447750 |
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| author | Khandaker Asif Ahmed Anjana Karawita Melissa J. Klein Luana Fiorella Mincarelli Barbara Secondini Giuseppe Satta Massimo Ancora Cipriano Foxi Marco Di Domenico Michela Quaglia Maria Goffredo Alessio Lorusso Cesare Cammà Leon Court Rahul V. Rane Tom K. Walsh Prasad N. Paradkar Debbie Eagles Gunjan Pandey Christopher M. Hardy |
| author_facet | Khandaker Asif Ahmed Anjana Karawita Melissa J. Klein Luana Fiorella Mincarelli Barbara Secondini Giuseppe Satta Massimo Ancora Cipriano Foxi Marco Di Domenico Michela Quaglia Maria Goffredo Alessio Lorusso Cesare Cammà Leon Court Rahul V. Rane Tom K. Walsh Prasad N. Paradkar Debbie Eagles Gunjan Pandey Christopher M. Hardy |
| author_sort | Khandaker Asif Ahmed |
| collection | DOAJ |
| description | Biting midges (Culicoides spp.) are important vectors of several insect borne arboviruses but are underrepresented in terms of availability of high-resolution genomic resources. We assembled and annotated complete mitochondrial genomes for two Culicoides species, namely C. brevitarsis and C. imicola which are proven vectors for Bluetongue Virus (BTV). We used both long read and short read sequencing technologies to assemble the circular genomes. The genome sizes are 17,100 bp and 17,031 bp, respectively, all comprising 37 genes, including 13 protein, 22 tRNA, two rRNA coding genes, and one non-coding AT rich control region. The gene organizations and orientations are comparable to other available Culicoides mitogenomes, except for a translocation of ND2 and six tRNA genes in both C. brevitarsis and C. imicola. Eleven protein-coding genes encode a full TAA stop codon, while two (ND5, COX3) are completed by mRNA polyadenylation. Phylogenetic analysis of the mitogenomes showed C. brevitarsis and C. imicola form a monophyletic group. The sequences of these mitogenomes contribute to a baseline of molecular tools for diagnostics and surveillance for use by World Organisation for Animal Health (WOAH) reference laboratories for monitoring vectors of emerging diseases. |
| format | Article |
| id | doaj-art-047cccc36ccb420182bac0b59e4d3045 |
| institution | Kabale University |
| issn | 2380-2359 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Mitochondrial DNA. Part B. Resources |
| spelling | doaj-art-047cccc36ccb420182bac0b59e4d30452025-01-09T05:23:23ZengTaylor & Francis GroupMitochondrial DNA. Part B. Resources2380-23592025-01-01101677110.1080/23802359.2024.2447750Complete mitochondrial genomes of Culicoides brevitarsis and Culicoides imicola biting midge vectors of Bluetongue VirusKhandaker Asif Ahmed0Anjana Karawita1Melissa J. Klein2Luana Fiorella Mincarelli3Barbara Secondini4Giuseppe Satta5Massimo Ancora6Cipriano Foxi7Marco Di Domenico8Michela Quaglia9Maria Goffredo10Alessio Lorusso11Cesare Cammà12Leon Court13Rahul V. Rane14Tom K. Walsh15Prasad N. Paradkar16Debbie Eagles17Gunjan Pandey18Christopher M. Hardy19CSIRO Australian Animal Health Laboratory (AAHL), Australian Centre for Disease Preparedness (ACDP), East Geelong, VIC, AustraliaCSIRO Australian Animal Health Laboratory (AAHL), Australian Centre for Disease Preparedness (ACDP), East Geelong, VIC, AustraliaCSIRO Health and Biosecurity (H&B), Australian Centre for Disease Preparedness (ACDP), East Geelong, VIC, AustraliaNational Reference Centre for Whole Genome Sequencing of microbial pathogens – Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise, Teramo, ItalyNational Reference Centre for Whole Genome Sequencing of microbial pathogens – Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise, Teramo, ItalyIstituto Zooprofilattico Sperimentale Della Sardegna, Sassari, ItalyNational Reference Centre for Whole Genome Sequencing of microbial pathogens – Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise, Teramo, ItalyIstituto Zooprofilattico Sperimentale Della Sardegna, Sassari, ItalyNational Reference Centre for Whole Genome Sequencing of microbial pathogens – Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise, Teramo, ItalyNational Reference Centre for Whole Genome Sequencing of microbial pathogens – Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise, Teramo, ItalyNational Reference Centre for Whole Genome Sequencing of microbial pathogens – Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise, Teramo, ItalyNational Reference Centre for Whole Genome Sequencing of microbial pathogens – Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise, Teramo, ItalyNational Reference Centre for Whole Genome Sequencing of microbial pathogens – Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise, Teramo, ItalyCSIRO Environment, Black Mountain, ACT, AustraliaCSIRO Health and Biosecurity (H&B), Parkville, VIC, AustraliaCSIRO Environment, Black Mountain, ACT, AustraliaCSIRO Health and Biosecurity (H&B), Australian Centre for Disease Preparedness (ACDP), East Geelong, VIC, AustraliaCSIRO Australian Animal Health Laboratory (AAHL), Australian Centre for Disease Preparedness (ACDP), East Geelong, VIC, AustraliaCSIRO Environment, Black Mountain, ACT, AustraliaCSIRO Environment, Black Mountain, ACT, AustraliaBiting midges (Culicoides spp.) are important vectors of several insect borne arboviruses but are underrepresented in terms of availability of high-resolution genomic resources. We assembled and annotated complete mitochondrial genomes for two Culicoides species, namely C. brevitarsis and C. imicola which are proven vectors for Bluetongue Virus (BTV). We used both long read and short read sequencing technologies to assemble the circular genomes. The genome sizes are 17,100 bp and 17,031 bp, respectively, all comprising 37 genes, including 13 protein, 22 tRNA, two rRNA coding genes, and one non-coding AT rich control region. The gene organizations and orientations are comparable to other available Culicoides mitogenomes, except for a translocation of ND2 and six tRNA genes in both C. brevitarsis and C. imicola. Eleven protein-coding genes encode a full TAA stop codon, while two (ND5, COX3) are completed by mRNA polyadenylation. Phylogenetic analysis of the mitogenomes showed C. brevitarsis and C. imicola form a monophyletic group. The sequences of these mitogenomes contribute to a baseline of molecular tools for diagnostics and surveillance for use by World Organisation for Animal Health (WOAH) reference laboratories for monitoring vectors of emerging diseases.https://www.tandfonline.com/doi/10.1080/23802359.2024.2447750Arbovirus vectorCulicoidesCeratopogonidaebiting midgesmitogenome |
| spellingShingle | Khandaker Asif Ahmed Anjana Karawita Melissa J. Klein Luana Fiorella Mincarelli Barbara Secondini Giuseppe Satta Massimo Ancora Cipriano Foxi Marco Di Domenico Michela Quaglia Maria Goffredo Alessio Lorusso Cesare Cammà Leon Court Rahul V. Rane Tom K. Walsh Prasad N. Paradkar Debbie Eagles Gunjan Pandey Christopher M. Hardy Complete mitochondrial genomes of Culicoides brevitarsis and Culicoides imicola biting midge vectors of Bluetongue Virus Mitochondrial DNA. Part B. Resources Arbovirus vector Culicoides Ceratopogonidae biting midges mitogenome |
| title | Complete mitochondrial genomes of Culicoides brevitarsis and Culicoides imicola biting midge vectors of Bluetongue Virus |
| title_full | Complete mitochondrial genomes of Culicoides brevitarsis and Culicoides imicola biting midge vectors of Bluetongue Virus |
| title_fullStr | Complete mitochondrial genomes of Culicoides brevitarsis and Culicoides imicola biting midge vectors of Bluetongue Virus |
| title_full_unstemmed | Complete mitochondrial genomes of Culicoides brevitarsis and Culicoides imicola biting midge vectors of Bluetongue Virus |
| title_short | Complete mitochondrial genomes of Culicoides brevitarsis and Culicoides imicola biting midge vectors of Bluetongue Virus |
| title_sort | complete mitochondrial genomes of culicoides brevitarsis and culicoides imicola biting midge vectors of bluetongue virus |
| topic | Arbovirus vector Culicoides Ceratopogonidae biting midges mitogenome |
| url | https://www.tandfonline.com/doi/10.1080/23802359.2024.2447750 |
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