Enrichment of Vigna unguiculata (L.) Walp sprouts with iron and zinc: influence on antioxidant capacity and phytochemicals
Micronutrients are essential for the growth and development of animals, plants, and humans. Crop biofortification has emerged as a viable strategy to address iron (Fe) and zinc (Zn) deficiencies in the soil. Biofortification with Fe and Zn during seed germination is particularly valuable, as it not...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Society of Land Measurements and Cadastre from Transylvania (SMTCT)
2025-03-01
|
| Series: | Notulae Scientia Biologicae |
| Subjects: | |
| Online Access: | https://www.notulaebiologicae.ro/index.php/nsb/article/view/12299 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849744748633391104 |
|---|---|
| author | Amelio E. MORALES-MORALES Julia MEDRANO-MACÍAS Efraín DE-LA-CRUZ-LÁZARO Rodolfo OSORIO-OSORIO José R. VELÁZQUEZ-MARTÍNEZ César MÁRQUEZ-QUIROZ |
| author_facet | Amelio E. MORALES-MORALES Julia MEDRANO-MACÍAS Efraín DE-LA-CRUZ-LÁZARO Rodolfo OSORIO-OSORIO José R. VELÁZQUEZ-MARTÍNEZ César MÁRQUEZ-QUIROZ |
| author_sort | Amelio E. MORALES-MORALES |
| collection | DOAJ |
| description |
Micronutrients are essential for the growth and development of animals, plants, and humans. Crop biofortification has emerged as a viable strategy to address iron (Fe) and zinc (Zn) deficiencies in the soil. Biofortification with Fe and Zn during seed germination is particularly valuable, as it not only meets nutritional requirements but also helps prevent diseases associated with Fe and Zn deficiencies. This study aimed to evaluate the antioxidant capacity and phytochemical content of cowpea sprouts (Vigna unguiculata L.) biofortified with Fe and Zn. Cowpea shoots were cultivated in a growth chamber under dark conditions with controlled temperature and water supply, and four concentrations of iron and zinc (0-, 5-, 10-, and 15-mM L⁻¹) were applied. The results indicated that optimizing the Fe and Zn concentrations was necessary to enhance the antioxidant capacity in the sprouts while avoiding stress from excessive nutrient levels. The low Zn concentration (5 mM) in the absence of Fe proved most effective in increasing antioxidant capacity, reaching 196.18 µg mL⁻¹. Zn application alone or at reduced Fe concentrations promoted a higher phenolic content in the sprouts. Additionally, the combination of 5 mM Fe with 15 mM Zn resulted in the highest condensed tannin content (102.67 mg catechin g⁻¹), whereas the combination of 15 mM Fe with 0 mM Zn produced the lowest phytic acid level at 0.32 mg FA g⁻¹.
|
| format | Article |
| id | doaj-art-047a836c4aca48cca9d8dc999d854bbd |
| institution | DOAJ |
| issn | 2067-3264 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Society of Land Measurements and Cadastre from Transylvania (SMTCT) |
| record_format | Article |
| series | Notulae Scientia Biologicae |
| spelling | doaj-art-047a836c4aca48cca9d8dc999d854bbd2025-08-20T03:09:45ZengSociety of Land Measurements and Cadastre from Transylvania (SMTCT)Notulae Scientia Biologicae2067-32642025-03-0117110.55779/nsb17112299Enrichment of Vigna unguiculata (L.) Walp sprouts with iron and zinc: influence on antioxidant capacity and phytochemicalsAmelio E. MORALES-MORALES0Julia MEDRANO-MACÍAS1Efraín DE-LA-CRUZ-LÁZARO2Rodolfo OSORIO-OSORIO3José R. VELÁZQUEZ-MARTÍNEZ4César MÁRQUEZ-QUIROZ5Universidad Juárez Autónoma de Tabasco, División Académica de Ciencias Agropecuarias, Carretera Villahermosa-Teapa km 25 R/a La Huasteca 2a sección, C.P. 86280. Centro, TabascoUniversidad Autónoma Agraria Antonio Narro, Departamento de Horticultura, Calzada Antonio Narro. Buenavista, Saltillo 25315Universidad Juárez Autónoma de Tabasco, División Académica de Ciencias Agropecuarias, Carretera Villahermosa-Teapa km 25 R/a La Huasteca 2a sección, C.P. 86280. Centro, TabascoUniversidad Juárez Autónoma de Tabasco, División Académica de Ciencias Agropecuarias, Carretera Villahermosa-Teapa km 25 R/a La Huasteca 2a sección, C.P. 86280. Centro, TabascoUniversidad Juárez Autónoma de Tabasco, División Académica de Ciencias Agropecuarias, Carretera Villahermosa-Teapa km 25 R/a La Huasteca 2a sección, C.P. 86280. Centro, TabascoUniversidad Juárez Autónoma de Tabasco, División Académica de Ciencias Agropecuarias, Carretera Villahermosa-Teapa km 25 R/a La Huasteca 2a sección, C.P. 86280. Centro, Tabasco Micronutrients are essential for the growth and development of animals, plants, and humans. Crop biofortification has emerged as a viable strategy to address iron (Fe) and zinc (Zn) deficiencies in the soil. Biofortification with Fe and Zn during seed germination is particularly valuable, as it not only meets nutritional requirements but also helps prevent diseases associated with Fe and Zn deficiencies. This study aimed to evaluate the antioxidant capacity and phytochemical content of cowpea sprouts (Vigna unguiculata L.) biofortified with Fe and Zn. Cowpea shoots were cultivated in a growth chamber under dark conditions with controlled temperature and water supply, and four concentrations of iron and zinc (0-, 5-, 10-, and 15-mM L⁻¹) were applied. The results indicated that optimizing the Fe and Zn concentrations was necessary to enhance the antioxidant capacity in the sprouts while avoiding stress from excessive nutrient levels. The low Zn concentration (5 mM) in the absence of Fe proved most effective in increasing antioxidant capacity, reaching 196.18 µg mL⁻¹. Zn application alone or at reduced Fe concentrations promoted a higher phenolic content in the sprouts. Additionally, the combination of 5 mM Fe with 15 mM Zn resulted in the highest condensed tannin content (102.67 mg catechin g⁻¹), whereas the combination of 15 mM Fe with 0 mM Zn produced the lowest phytic acid level at 0.32 mg FA g⁻¹. https://www.notulaebiologicae.ro/index.php/nsb/article/view/12299cowpeaphytic acidradical scavenging activitytanninstotal phenols |
| spellingShingle | Amelio E. MORALES-MORALES Julia MEDRANO-MACÍAS Efraín DE-LA-CRUZ-LÁZARO Rodolfo OSORIO-OSORIO José R. VELÁZQUEZ-MARTÍNEZ César MÁRQUEZ-QUIROZ Enrichment of Vigna unguiculata (L.) Walp sprouts with iron and zinc: influence on antioxidant capacity and phytochemicals Notulae Scientia Biologicae cowpea phytic acid radical scavenging activity tannins total phenols |
| title | Enrichment of Vigna unguiculata (L.) Walp sprouts with iron and zinc: influence on antioxidant capacity and phytochemicals |
| title_full | Enrichment of Vigna unguiculata (L.) Walp sprouts with iron and zinc: influence on antioxidant capacity and phytochemicals |
| title_fullStr | Enrichment of Vigna unguiculata (L.) Walp sprouts with iron and zinc: influence on antioxidant capacity and phytochemicals |
| title_full_unstemmed | Enrichment of Vigna unguiculata (L.) Walp sprouts with iron and zinc: influence on antioxidant capacity and phytochemicals |
| title_short | Enrichment of Vigna unguiculata (L.) Walp sprouts with iron and zinc: influence on antioxidant capacity and phytochemicals |
| title_sort | enrichment of vigna unguiculata l walp sprouts with iron and zinc influence on antioxidant capacity and phytochemicals |
| topic | cowpea phytic acid radical scavenging activity tannins total phenols |
| url | https://www.notulaebiologicae.ro/index.php/nsb/article/view/12299 |
| work_keys_str_mv | AT amelioemoralesmorales enrichmentofvignaunguiculatalwalpsproutswithironandzincinfluenceonantioxidantcapacityandphytochemicals AT juliamedranomacias enrichmentofvignaunguiculatalwalpsproutswithironandzincinfluenceonantioxidantcapacityandphytochemicals AT efraindelacruzlazaro enrichmentofvignaunguiculatalwalpsproutswithironandzincinfluenceonantioxidantcapacityandphytochemicals AT rodolfoosorioosorio enrichmentofvignaunguiculatalwalpsproutswithironandzincinfluenceonantioxidantcapacityandphytochemicals AT joservelazquezmartinez enrichmentofvignaunguiculatalwalpsproutswithironandzincinfluenceonantioxidantcapacityandphytochemicals AT cesarmarquezquiroz enrichmentofvignaunguiculatalwalpsproutswithironandzincinfluenceonantioxidantcapacityandphytochemicals |