Anti-Inflammatory Activity of Artemisia vulgaris Leaves, Originating from Three Different Altitudes of Nepal
This study aimed to evaluate and compare the in vivo chronic anti-inflammatory efficacy, from the ethyl acetate and ethanolic extracts of Artemisia vulgaris leaves, grown at three different altitudes in Nepal, by formalin-induced paw edema in Swiss albino mice. Edema was induced on the mice paw by a...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2021/6678059 |
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| author | Jitendra Pandey Sushma Bhusal Laxman Nepali Maya Khatri Rasmita Ramdam Himal Barakoti Paras Mani Giri Dhakaraj Pant Pramod Aryal Rabindra Kumar Rokaya Ravin Bhandari |
| author_facet | Jitendra Pandey Sushma Bhusal Laxman Nepali Maya Khatri Rasmita Ramdam Himal Barakoti Paras Mani Giri Dhakaraj Pant Pramod Aryal Rabindra Kumar Rokaya Ravin Bhandari |
| author_sort | Jitendra Pandey |
| collection | DOAJ |
| description | This study aimed to evaluate and compare the in vivo chronic anti-inflammatory efficacy, from the ethyl acetate and ethanolic extracts of Artemisia vulgaris leaves, grown at three different altitudes in Nepal, by formalin-induced paw edema in Swiss albino mice. Edema was induced on the mice paw by administering 0.2% of formalin injection. Indomethacin was used as a standard drug at the concentration of 5 mg/kg of body weight. Ethyl acetate and ethanolic leaves extract, at the concentration of 200 mg/kg and 400 mg/kg, were used as test drugs. Standard drug and all the extracts were administered 30 min before formalin injection. The paw thickness was measured at 0, 1, 2, 3, 24, 48, and 72 hours after formalin injection, using a Vernier caliper. It was observed that both ethyl acetate and ethanolic extract from all the altitudes exhibited significant inhibition of paw edema (p<0.05) induced by formalin. Maximum activity was shown by 400 mg/kg of the plant leaf extract taken from the temperate zone, with 54.05% of paw edema inhibition, and it is almost similar to the inhibition of standard drug (56.75%). Moreover, the ethanolic extract was found to be more effective than ethyl acetate extract in all the plant samples. The results suggested that the anti-inflammatory effect of A. vulgaris leaves increases with an increase in altitudes and this plant can be used as a useful source of medicine to treat chronic inflammation. |
| format | Article |
| id | doaj-art-045e7cfc4f684e32b02287c75884b8a4 |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-045e7cfc4f684e32b02287c75884b8a42025-08-20T03:55:23ZengWileyThe Scientific World Journal2356-61401537-744X2021-01-01202110.1155/2021/66780596678059Anti-Inflammatory Activity of Artemisia vulgaris Leaves, Originating from Three Different Altitudes of NepalJitendra Pandey0Sushma Bhusal1Laxman Nepali2Maya Khatri3Rasmita Ramdam4Himal Barakoti5Paras Mani Giri6Dhakaraj Pant7Pramod Aryal8Rabindra Kumar Rokaya9Ravin Bhandari10Department of Pharmacy, Crimson College of Technology, Affiliated to Pokhara University, Butwal, Rupandehi 32900, NepalDepartment of Pharmacy, Crimson College of Technology, Affiliated to Pokhara University, Butwal, Rupandehi 32900, NepalDepartment of Pharmacy, Crimson College of Technology, Affiliated to Pokhara University, Butwal, Rupandehi 32900, NepalDepartment of Pharmacy, Crimson College of Technology, Affiliated to Pokhara University, Butwal, Rupandehi 32900, NepalDepartment of Pharmacy, Crimson College of Technology, Affiliated to Pokhara University, Butwal, Rupandehi 32900, NepalDepartment of Pharmacy, Purbanchal University, Biratnagar 56613, NepalSiddhartha Pharmaceutical Private Limited, Rupandehi 32900, NepalDepartment of Medical Laboratory Science, School of Health and Allied Sciences, Pokhara University, Pokhara 33370, NepalDepartment of Pharmacy, Crimson College of Technology, Affiliated to Pokhara University, Butwal, Rupandehi 32900, NepalDepartment of Pharmacology, Karnali Academy of Health Science, Jumla, Chandannath 21200, NepalDepartment of Pharmacy, Crimson College of Technology, Affiliated to Pokhara University, Butwal, Rupandehi 32900, NepalThis study aimed to evaluate and compare the in vivo chronic anti-inflammatory efficacy, from the ethyl acetate and ethanolic extracts of Artemisia vulgaris leaves, grown at three different altitudes in Nepal, by formalin-induced paw edema in Swiss albino mice. Edema was induced on the mice paw by administering 0.2% of formalin injection. Indomethacin was used as a standard drug at the concentration of 5 mg/kg of body weight. Ethyl acetate and ethanolic leaves extract, at the concentration of 200 mg/kg and 400 mg/kg, were used as test drugs. Standard drug and all the extracts were administered 30 min before formalin injection. The paw thickness was measured at 0, 1, 2, 3, 24, 48, and 72 hours after formalin injection, using a Vernier caliper. It was observed that both ethyl acetate and ethanolic extract from all the altitudes exhibited significant inhibition of paw edema (p<0.05) induced by formalin. Maximum activity was shown by 400 mg/kg of the plant leaf extract taken from the temperate zone, with 54.05% of paw edema inhibition, and it is almost similar to the inhibition of standard drug (56.75%). Moreover, the ethanolic extract was found to be more effective than ethyl acetate extract in all the plant samples. The results suggested that the anti-inflammatory effect of A. vulgaris leaves increases with an increase in altitudes and this plant can be used as a useful source of medicine to treat chronic inflammation.http://dx.doi.org/10.1155/2021/6678059 |
| spellingShingle | Jitendra Pandey Sushma Bhusal Laxman Nepali Maya Khatri Rasmita Ramdam Himal Barakoti Paras Mani Giri Dhakaraj Pant Pramod Aryal Rabindra Kumar Rokaya Ravin Bhandari Anti-Inflammatory Activity of Artemisia vulgaris Leaves, Originating from Three Different Altitudes of Nepal The Scientific World Journal |
| title | Anti-Inflammatory Activity of Artemisia vulgaris Leaves, Originating from Three Different Altitudes of Nepal |
| title_full | Anti-Inflammatory Activity of Artemisia vulgaris Leaves, Originating from Three Different Altitudes of Nepal |
| title_fullStr | Anti-Inflammatory Activity of Artemisia vulgaris Leaves, Originating from Three Different Altitudes of Nepal |
| title_full_unstemmed | Anti-Inflammatory Activity of Artemisia vulgaris Leaves, Originating from Three Different Altitudes of Nepal |
| title_short | Anti-Inflammatory Activity of Artemisia vulgaris Leaves, Originating from Three Different Altitudes of Nepal |
| title_sort | anti inflammatory activity of artemisia vulgaris leaves originating from three different altitudes of nepal |
| url | http://dx.doi.org/10.1155/2021/6678059 |
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