Development and Validation of a Novel Kinetic Spectrophotometric Method for Determining N-acetyl-L-cysteine
N-acetyl-L-cysteine (NAC) is a precursor of glutathione and a natural antioxidant. It is used to treat paracetamol overdose and to dissolve thick bronchial mucus. A novel, simple, reproducible, and rapid kinetic spectrophotometric method for determining NAC in pharmaceutical formulations is proposed...
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Croatian Society of Chemical Engineers
2025-05-01
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| Series: | Kemija u Industriji |
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| Online Access: | http://silverstripe.fkit.hr/kui/assets/Uploads/3-209-217-KUI-5-6-2025.pdf |
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| author | Maja Biočić Šormaz Tomislav Kraljević Lea Kukoč-Modun |
| author_facet | Maja Biočić Šormaz Tomislav Kraljević Lea Kukoč-Modun |
| author_sort | Maja Biočić Šormaz |
| collection | DOAJ |
| description | N-acetyl-L-cysteine (NAC) is a precursor of glutathione and a natural antioxidant. It is used to treat paracetamol overdose and to dissolve thick bronchial mucus. A novel, simple, reproducible, and rapid kinetic spectrophotometric method for determining NAC in pharmaceutical formulations is proposed. The method is based on a redox reaction where NAC reduces bis(bathocuproinedisulfonate)cuprate(II), forming a yellow-orange complex of bis(bathocuproinedisulfonate)cuprate(I). The resulting stable complex exhibited maximum absorbance at λ = 483 nm. Under optimised chemical reaction parameters, both the fixed-time and initial-rate methods were used to generate calibration curves. For the fixed-time method, the curve was linear in the concentration range of 3.0 · 10−7 to 7.0 · 10−5 mol l−1, with the equation y = 13140x − 0.0005 and a coefficient of determination of R2= 0.9999. For the initial-rate method, the curve was linear in the concentration range of 7.0 · 10−7 to 7.0 · 10−5 mol l−1 with the equation y = 1.040x + 3.220 and R2 = 0.9991. The slope of the logarithmic form of the calibration curve (1.040) for the initial-rate method confirmed the pseudo-first order nature of the reaction. The proposed method was successfully applied for the determination of NAC in commercial pharmaceutical preparations, yielding results comparable to those obtained using the recommended method according to the pharmacopoeia. |
| format | Article |
| id | doaj-art-04472caa2d364e99854d292d7e515538 |
| institution | OA Journals |
| issn | 0022-9830 1334-9090 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Croatian Society of Chemical Engineers |
| record_format | Article |
| series | Kemija u Industriji |
| spelling | doaj-art-04472caa2d364e99854d292d7e5155382025-08-20T02:30:47ZengCroatian Society of Chemical EngineersKemija u Industriji0022-98301334-90902025-05-01745-620921710.15255/KUI.2024.046Development and Validation of a Novel Kinetic Spectrophotometric Method for Determining N-acetyl-L-cysteineMaja Biočić Šormaz0Tomislav Kraljević1Lea Kukoč-Modun2Department of Analytical and Environmental Chemistry, Faculty of Chemistry and Technology, University of Split, Rudjera Boškovića 35, 21000 Split, CroatiaDepartment of Chemistry, Faculty of Science and Education, University of Mostar, Matice hrvatske bb, 88000 Mostar, Bosnia and HerzegovinaDepartment of Analytical and Environmental Chemistry, Faculty of Chemistry and Technology, University of Split, Rudjera Boškovića 35, 21000 Split, CroatiaN-acetyl-L-cysteine (NAC) is a precursor of glutathione and a natural antioxidant. It is used to treat paracetamol overdose and to dissolve thick bronchial mucus. A novel, simple, reproducible, and rapid kinetic spectrophotometric method for determining NAC in pharmaceutical formulations is proposed. The method is based on a redox reaction where NAC reduces bis(bathocuproinedisulfonate)cuprate(II), forming a yellow-orange complex of bis(bathocuproinedisulfonate)cuprate(I). The resulting stable complex exhibited maximum absorbance at λ = 483 nm. Under optimised chemical reaction parameters, both the fixed-time and initial-rate methods were used to generate calibration curves. For the fixed-time method, the curve was linear in the concentration range of 3.0 · 10−7 to 7.0 · 10−5 mol l−1, with the equation y = 13140x − 0.0005 and a coefficient of determination of R2= 0.9999. For the initial-rate method, the curve was linear in the concentration range of 7.0 · 10−7 to 7.0 · 10−5 mol l−1 with the equation y = 1.040x + 3.220 and R2 = 0.9991. The slope of the logarithmic form of the calibration curve (1.040) for the initial-rate method confirmed the pseudo-first order nature of the reaction. The proposed method was successfully applied for the determination of NAC in commercial pharmaceutical preparations, yielding results comparable to those obtained using the recommended method according to the pharmacopoeia.http://silverstripe.fkit.hr/kui/assets/Uploads/3-209-217-KUI-5-6-2025.pdfkineticsspectrophotometryn-acetyl-l-cysteinebathocuproine disulfonateredox reaction |
| spellingShingle | Maja Biočić Šormaz Tomislav Kraljević Lea Kukoč-Modun Development and Validation of a Novel Kinetic Spectrophotometric Method for Determining N-acetyl-L-cysteine Kemija u Industriji kinetics spectrophotometry n-acetyl-l-cysteine bathocuproine disulfonate redox reaction |
| title | Development and Validation of a Novel Kinetic Spectrophotometric Method for Determining N-acetyl-L-cysteine |
| title_full | Development and Validation of a Novel Kinetic Spectrophotometric Method for Determining N-acetyl-L-cysteine |
| title_fullStr | Development and Validation of a Novel Kinetic Spectrophotometric Method for Determining N-acetyl-L-cysteine |
| title_full_unstemmed | Development and Validation of a Novel Kinetic Spectrophotometric Method for Determining N-acetyl-L-cysteine |
| title_short | Development and Validation of a Novel Kinetic Spectrophotometric Method for Determining N-acetyl-L-cysteine |
| title_sort | development and validation of a novel kinetic spectrophotometric method for determining n acetyl l cysteine |
| topic | kinetics spectrophotometry n-acetyl-l-cysteine bathocuproine disulfonate redox reaction |
| url | http://silverstripe.fkit.hr/kui/assets/Uploads/3-209-217-KUI-5-6-2025.pdf |
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