Comparison of the Effects of Different Cryoprotectants on Stem Cells from Umbilical Cord Blood
Purpose. Cryoprotectants (CPA) for stem cells from umbilical cord blood (UCB) have been widely developed based on empirical evidence, but there is no consensus on a standard protocol of preservation of the UCB cells. Methods. In this study, UCB from 115 donors was collected. Each unit of UCB was div...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/1396783 |
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| author | Gecai Chen Aihuan Yue Zhongbao Ruan Yigang Yin Ruzhu Wang Yin Ren Li Zhu |
| author_facet | Gecai Chen Aihuan Yue Zhongbao Ruan Yigang Yin Ruzhu Wang Yin Ren Li Zhu |
| author_sort | Gecai Chen |
| collection | DOAJ |
| description | Purpose. Cryoprotectants (CPA) for stem cells from umbilical cord blood (UCB) have been widely developed based on empirical evidence, but there is no consensus on a standard protocol of preservation of the UCB cells. Methods. In this study, UCB from 115 donors was collected. Each unit of UCB was divided into four equal parts and frozen in different kinds of cryoprotectant as follows: group A, 10% ethylene glycol and 2.0% dimethyl sulfoxide (DMSO) (v/v); group B, 10% DMSO and 2.0% dextran-40; group C, 2.5% DMSO (v/v) + 30 mmol/L trehalose; and group D, without CPA. Results. CD34+, cell viability, colony forming units (CFUs), and cell apoptosis of pre- and postcryopreservation using three cryoprotectants were analyzed. After thawing, significant differences in CD34+ count, CFUs, cell apoptosis, and cell viability were observed among the four groups (P<0.05). Conclusion. The low concentration of DMSO with the addition of trehalose might improve the cryopreservation outcome. |
| format | Article |
| id | doaj-art-043b8957b08941b08ca251c60e763740 |
| institution | OA Journals |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-043b8957b08941b08ca251c60e7637402025-08-20T02:03:06ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/13967831396783Comparison of the Effects of Different Cryoprotectants on Stem Cells from Umbilical Cord BloodGecai Chen0Aihuan Yue1Zhongbao Ruan2Yigang Yin3Ruzhu Wang4Yin Ren5Li Zhu6Department of Cardiology, Taizhou People’s Hospital, 399 Hailing Road, Taizhou, Jiangsu 225300, ChinaStem Cell Research Center, 399 Hailing Road, Taizhou, Jiangsu 225300, ChinaDepartment of Cardiology, Taizhou People’s Hospital, 399 Hailing Road, Taizhou, Jiangsu 225300, ChinaDepartment of Cardiology, Taizhou People’s Hospital, 399 Hailing Road, Taizhou, Jiangsu 225300, ChinaDepartment of Cardiology, Taizhou People’s Hospital, 399 Hailing Road, Taizhou, Jiangsu 225300, ChinaDepartment of Cardiology, Taizhou People’s Hospital, 399 Hailing Road, Taizhou, Jiangsu 225300, ChinaDepartment of Cardiology, Taizhou People’s Hospital, 399 Hailing Road, Taizhou, Jiangsu 225300, ChinaPurpose. Cryoprotectants (CPA) for stem cells from umbilical cord blood (UCB) have been widely developed based on empirical evidence, but there is no consensus on a standard protocol of preservation of the UCB cells. Methods. In this study, UCB from 115 donors was collected. Each unit of UCB was divided into four equal parts and frozen in different kinds of cryoprotectant as follows: group A, 10% ethylene glycol and 2.0% dimethyl sulfoxide (DMSO) (v/v); group B, 10% DMSO and 2.0% dextran-40; group C, 2.5% DMSO (v/v) + 30 mmol/L trehalose; and group D, without CPA. Results. CD34+, cell viability, colony forming units (CFUs), and cell apoptosis of pre- and postcryopreservation using three cryoprotectants were analyzed. After thawing, significant differences in CD34+ count, CFUs, cell apoptosis, and cell viability were observed among the four groups (P<0.05). Conclusion. The low concentration of DMSO with the addition of trehalose might improve the cryopreservation outcome.http://dx.doi.org/10.1155/2016/1396783 |
| spellingShingle | Gecai Chen Aihuan Yue Zhongbao Ruan Yigang Yin Ruzhu Wang Yin Ren Li Zhu Comparison of the Effects of Different Cryoprotectants on Stem Cells from Umbilical Cord Blood Stem Cells International |
| title | Comparison of the Effects of Different Cryoprotectants on Stem Cells from Umbilical Cord Blood |
| title_full | Comparison of the Effects of Different Cryoprotectants on Stem Cells from Umbilical Cord Blood |
| title_fullStr | Comparison of the Effects of Different Cryoprotectants on Stem Cells from Umbilical Cord Blood |
| title_full_unstemmed | Comparison of the Effects of Different Cryoprotectants on Stem Cells from Umbilical Cord Blood |
| title_short | Comparison of the Effects of Different Cryoprotectants on Stem Cells from Umbilical Cord Blood |
| title_sort | comparison of the effects of different cryoprotectants on stem cells from umbilical cord blood |
| url | http://dx.doi.org/10.1155/2016/1396783 |
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