Application of single-cell sequencing technology to the study of embryonic developmental toxicity of emerging contaminants: Progress and perspectives
Emerging pollutants are a class of environmental chemicals characterized by bioaccumulation, environmental persistence, and high toxicity. Their long-term accumulation poses severe threats to the environment and human health. In recent years, single-cell RNA sequencing (scRNA-seq) technology has ena...
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Editorial Committee of Journal of Environmental and Occupational Medicine
2024-12-01
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| Series: | 环境与职业医学 |
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| Online Access: | http://www.jeom.org/article/cn/10.11836/JEOM24390 |
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| author | Weizhao CAO Yunhui ZHANG |
| author_facet | Weizhao CAO Yunhui ZHANG |
| author_sort | Weizhao CAO |
| collection | DOAJ |
| description | Emerging pollutants are a class of environmental chemicals characterized by bioaccumulation, environmental persistence, and high toxicity. Their long-term accumulation poses severe threats to the environment and human health. In recent years, single-cell RNA sequencing (scRNA-seq) technology has enabled the analysis of transcriptomes at the single-cell level, revealing cellular heterogeneity and the complexity of intercellular communication. In toxicological studies, scRNA-seq technology has been applied to identify the responses of different cell types to pollutants and to elucidate the mechanisms of pollutant action during embryonic development, providing novel perspectives on the toxicity mechanisms of emerging pollutants. This paper reviewed the classification and characteristics of single-cell sequencing technologies and their applications in investigating the developmental toxicity of emerging pollutants, including perfluorinated and polyfluorinated alkyl substances (PFAS), antibiotics, microplastics, phthalates, and bisphenols. It also discussed the limitations of current studies and proposed future research directions. Through methods such as cellular heterogeneity analysis and pseudotemporal chronological analysis, single-cell sequencing can explore and validate the specific toxicity mechanisms of emerging pollutants in embryonic development. However, challenges remain, including dataset bias, batch effects, and developmental differences between humans and model organisms. Future research should focus on optimizing data analysis methods, integrating multi-omics sequencing data, and exploring the use of human organoid models in toxicological studies. Such efforts will contribute to a more comprehensive understanding of the toxicity mechanisms and long-term health impacts of emerging pollutants, providing a theoretical basis for their stringent regulation and control. |
| format | Article |
| id | doaj-art-043907f5f4df4a9391240d39f2d93e9d |
| institution | Kabale University |
| issn | 2095-9982 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Editorial Committee of Journal of Environmental and Occupational Medicine |
| record_format | Article |
| series | 环境与职业医学 |
| spelling | doaj-art-043907f5f4df4a9391240d39f2d93e9d2025-01-09T05:41:12ZengEditorial Committee of Journal of Environmental and Occupational Medicine环境与职业医学2095-99822024-12-0141121333134010.11836/JEOM2439024390Application of single-cell sequencing technology to the study of embryonic developmental toxicity of emerging contaminants: Progress and perspectivesWeizhao CAO0Yunhui ZHANG1School of Public Health/Key Laboratory of Public Health Safety, Ministry of Education, Fudan University, Shanghai 200032, ChinaSchool of Public Health/Key Laboratory of Public Health Safety, Ministry of Education, Fudan University, Shanghai 200032, ChinaEmerging pollutants are a class of environmental chemicals characterized by bioaccumulation, environmental persistence, and high toxicity. Their long-term accumulation poses severe threats to the environment and human health. In recent years, single-cell RNA sequencing (scRNA-seq) technology has enabled the analysis of transcriptomes at the single-cell level, revealing cellular heterogeneity and the complexity of intercellular communication. In toxicological studies, scRNA-seq technology has been applied to identify the responses of different cell types to pollutants and to elucidate the mechanisms of pollutant action during embryonic development, providing novel perspectives on the toxicity mechanisms of emerging pollutants. This paper reviewed the classification and characteristics of single-cell sequencing technologies and their applications in investigating the developmental toxicity of emerging pollutants, including perfluorinated and polyfluorinated alkyl substances (PFAS), antibiotics, microplastics, phthalates, and bisphenols. It also discussed the limitations of current studies and proposed future research directions. Through methods such as cellular heterogeneity analysis and pseudotemporal chronological analysis, single-cell sequencing can explore and validate the specific toxicity mechanisms of emerging pollutants in embryonic development. However, challenges remain, including dataset bias, batch effects, and developmental differences between humans and model organisms. Future research should focus on optimizing data analysis methods, integrating multi-omics sequencing data, and exploring the use of human organoid models in toxicological studies. Such efforts will contribute to a more comprehensive understanding of the toxicity mechanisms and long-term health impacts of emerging pollutants, providing a theoretical basis for their stringent regulation and control.http://www.jeom.org/article/cn/10.11836/JEOM24390emerging pollutantsingle-cell rna sequencingembryonic developmental toxicitycellular heterogeneityproposed chronological analysis |
| spellingShingle | Weizhao CAO Yunhui ZHANG Application of single-cell sequencing technology to the study of embryonic developmental toxicity of emerging contaminants: Progress and perspectives 环境与职业医学 emerging pollutant single-cell rna sequencing embryonic developmental toxicity cellular heterogeneity proposed chronological analysis |
| title | Application of single-cell sequencing technology to the study of embryonic developmental toxicity of emerging contaminants: Progress and perspectives |
| title_full | Application of single-cell sequencing technology to the study of embryonic developmental toxicity of emerging contaminants: Progress and perspectives |
| title_fullStr | Application of single-cell sequencing technology to the study of embryonic developmental toxicity of emerging contaminants: Progress and perspectives |
| title_full_unstemmed | Application of single-cell sequencing technology to the study of embryonic developmental toxicity of emerging contaminants: Progress and perspectives |
| title_short | Application of single-cell sequencing technology to the study of embryonic developmental toxicity of emerging contaminants: Progress and perspectives |
| title_sort | application of single cell sequencing technology to the study of embryonic developmental toxicity of emerging contaminants progress and perspectives |
| topic | emerging pollutant single-cell rna sequencing embryonic developmental toxicity cellular heterogeneity proposed chronological analysis |
| url | http://www.jeom.org/article/cn/10.11836/JEOM24390 |
| work_keys_str_mv | AT weizhaocao applicationofsinglecellsequencingtechnologytothestudyofembryonicdevelopmentaltoxicityofemergingcontaminantsprogressandperspectives AT yunhuizhang applicationofsinglecellsequencingtechnologytothestudyofembryonicdevelopmentaltoxicityofemergingcontaminantsprogressandperspectives |