Expression of the C-terminal 26 kDa fragment of Helicobacter pylori UreB in Escherichia coli and its immunity
A 720 bp fragment of ureB encoding the urease beta subunits of Helicobacter pylori was cloned into an expression vector (pAMJ399) and expressed in Escherichia coli JM109 cells. The recombinant rUreB-E fractions of UreB displayed molecular masses of approximate 26 kDa estimated by SDS-PAGE. Western b...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2006-07-01
|
| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2006.04.0387 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | A 720 bp fragment of ureB encoding the urease beta subunits of Helicobacter pylori was cloned into an expression vector (pAMJ399) and expressed in Escherichia coli JM109 cells. The recombinant rUreB-E fractions of UreB displayed molecular masses of approximate 26 kDa estimated by SDS-PAGE. Western blotting analysis indicated that the proteins were strongly immunogenic and were specifically recognized by polyclonal human anti-Helicobacter pylori sera. ELISA tests showed serum from Balb/c mice immunized with the recombinant protein was able to induce anti-UreB serum antibody, and that the protein qualified immunoreactivity and antigenicity. |
|---|---|
| ISSN: | 1008-9209 2097-5155 |