Long Noncoding RNA H19 Participates in the Regulation of Adipose-Derived Stem Cells Cartilage Differentiation
Adipose-derived stem cells (ADSCs) are multipotent and have received increasing attention for their applications in medicine. Cell-based therapies are optimal for diseases with loss or damage to tissues or organs. ADSCs and bone marrow mesenchymal stem cells (BMSCs) can differentiate into many cell...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2019/2139814 |
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| author | Hai-lin Pang Qian-qian Zhao Yu Ma Yong-li Song Jie Min Jian-rong Lu Hang Li Da-qing Zhao |
| author_facet | Hai-lin Pang Qian-qian Zhao Yu Ma Yong-li Song Jie Min Jian-rong Lu Hang Li Da-qing Zhao |
| author_sort | Hai-lin Pang |
| collection | DOAJ |
| description | Adipose-derived stem cells (ADSCs) are multipotent and have received increasing attention for their applications in medicine. Cell-based therapies are optimal for diseases with loss or damage to tissues or organs. ADSCs and bone marrow mesenchymal stem cells (BMSCs) can differentiate into many cell lineages. Because of their advantages in accessibility and volume, ADSCs are regarded as a desirable alternative to BMSCs. In this study, we focused on the chondrocytic differentiation potential of ADSCs and the underlying mechanism. We found that the long noncoding RNA H19 plays an important role in this process. Overexpression of H19 in ADSCs induced differentiation towards chondrocytes. H19 is abundantly expressed during embryonic development and downregulated after birth, implying its regulatory role in determining cell fate. However, in our experiments, H19 exerted its regulatory function during cartilage differentiation of ADSCs through competing miRNA regulation of STAT2. |
| format | Article |
| id | doaj-art-0413fcac661f4a16babf69c8c3db9f27 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-0413fcac661f4a16babf69c8c3db9f272025-08-20T03:54:15ZengWileyStem Cells International1687-966X1687-96782019-01-01201910.1155/2019/21398142139814Long Noncoding RNA H19 Participates in the Regulation of Adipose-Derived Stem Cells Cartilage DifferentiationHai-lin Pang0Qian-qian Zhao1Yu Ma2Yong-li Song3Jie Min4Jian-rong Lu5Hang Li6Da-qing Zhao7Department of Otolaryngology, Tangdu Hospital, The Air Force Medical University, 569 Xinsi Road, Xi’an 710038, ChinaDepartment of Otolaryngology, Tangdu Hospital, The Air Force Medical University, 569 Xinsi Road, Xi’an 710038, ChinaState Key Laboratory of Cancer Biology and Department of Pathology, Xijing Hospital, The Air Force Medical University, 127 Changle Western Road, Xi’an 710032, ChinaDepartment of Otolaryngology, Xijing Hospital, The Air Force Medical University, 127 Changle Western Road, Xi’an 710032, ChinaDepartment of Oncology, Tangdu Hospital, The Air Force Medical University, 569 Xinsi Road, Xi’an 710038, ChinaState Key Laboratory of Cancer Biology and Department of Pathology, Xijing Hospital, The Air Force Medical University, 127 Changle Western Road, Xi’an 710032, ChinaState Key Laboratory of Cancer Biology and Department of Pathology, Xijing Hospital, The Air Force Medical University, 127 Changle Western Road, Xi’an 710032, ChinaDepartment of Otolaryngology, Tangdu Hospital, The Air Force Medical University, 569 Xinsi Road, Xi’an 710038, ChinaAdipose-derived stem cells (ADSCs) are multipotent and have received increasing attention for their applications in medicine. Cell-based therapies are optimal for diseases with loss or damage to tissues or organs. ADSCs and bone marrow mesenchymal stem cells (BMSCs) can differentiate into many cell lineages. Because of their advantages in accessibility and volume, ADSCs are regarded as a desirable alternative to BMSCs. In this study, we focused on the chondrocytic differentiation potential of ADSCs and the underlying mechanism. We found that the long noncoding RNA H19 plays an important role in this process. Overexpression of H19 in ADSCs induced differentiation towards chondrocytes. H19 is abundantly expressed during embryonic development and downregulated after birth, implying its regulatory role in determining cell fate. However, in our experiments, H19 exerted its regulatory function during cartilage differentiation of ADSCs through competing miRNA regulation of STAT2.http://dx.doi.org/10.1155/2019/2139814 |
| spellingShingle | Hai-lin Pang Qian-qian Zhao Yu Ma Yong-li Song Jie Min Jian-rong Lu Hang Li Da-qing Zhao Long Noncoding RNA H19 Participates in the Regulation of Adipose-Derived Stem Cells Cartilage Differentiation Stem Cells International |
| title | Long Noncoding RNA H19 Participates in the Regulation of Adipose-Derived Stem Cells Cartilage Differentiation |
| title_full | Long Noncoding RNA H19 Participates in the Regulation of Adipose-Derived Stem Cells Cartilage Differentiation |
| title_fullStr | Long Noncoding RNA H19 Participates in the Regulation of Adipose-Derived Stem Cells Cartilage Differentiation |
| title_full_unstemmed | Long Noncoding RNA H19 Participates in the Regulation of Adipose-Derived Stem Cells Cartilage Differentiation |
| title_short | Long Noncoding RNA H19 Participates in the Regulation of Adipose-Derived Stem Cells Cartilage Differentiation |
| title_sort | long noncoding rna h19 participates in the regulation of adipose derived stem cells cartilage differentiation |
| url | http://dx.doi.org/10.1155/2019/2139814 |
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