A Novel Qualitative and Quantitative Biofilm Assay Based on 3D Soft Tissue
The lack of predictable in vitro methods to analyze antimicrobial activity could play a role in the development of resistance to antibiotics. Current used methods analyze planktonic cells but for the method to be clinically relevant, biofilm in in vivo like conditions ought to be studied. Hence, our...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | International Journal of Biomaterials |
| Online Access: | http://dx.doi.org/10.1155/2014/768136 |
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| author | Bodil Hakonen Linnea K. Lönnberg Eva Larkö Kristina Blom |
| author_facet | Bodil Hakonen Linnea K. Lönnberg Eva Larkö Kristina Blom |
| author_sort | Bodil Hakonen |
| collection | DOAJ |
| description | The lack of predictable in vitro methods to analyze antimicrobial activity could play a role in the development of resistance to antibiotics. Current used methods analyze planktonic cells but for the method to be clinically relevant, biofilm in in vivo like conditions ought to be studied. Hence, our group has developed a qualitative and quantitative method with in vivo like 3D tissue for prediction of antimicrobial activity in reality. Devices (wound dressings) were applied on top of Pseudomonas aeruginosa inoculated Muller-Hinton (MH) agar or 3D synthetic soft tissues (SST) and incubated for 24 hours. The antibacterial activity was then analyzed visually and by viable counts. On MH agar two out of three silver containing devices showed zone of inhibitions (ZOI) and on SST, ZOI were detected for all three. Corroborating results were found upon evaluating the bacterial load in SST and shown to be silver concentration dependent. In conclusion, a novel method was developed combining visual rapid screening and quantitative evaluation of the antimicrobial activity in both tissue and devices. It uses tissue allowing biofilm formation thus mimicking reality closely. These conditions are essential in order to predict antimicrobial activity of medical devices in the task to prevent device related infections. |
| format | Article |
| id | doaj-art-0408b3cdd1114268bf39290ab9c4e337 |
| institution | OA Journals |
| issn | 1687-8787 1687-8795 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Biomaterials |
| spelling | doaj-art-0408b3cdd1114268bf39290ab9c4e3372025-08-20T02:03:18ZengWileyInternational Journal of Biomaterials1687-87871687-87952014-01-01201410.1155/2014/768136768136A Novel Qualitative and Quantitative Biofilm Assay Based on 3D Soft TissueBodil Hakonen0Linnea K. Lönnberg1Eva Larkö2Kristina Blom3Medibiome AB, 431 53 Mölndal, SwedenMedibiome AB, 431 53 Mölndal, SwedenMedibiome AB, 431 53 Mölndal, SwedenMedibiome AB, 431 53 Mölndal, SwedenThe lack of predictable in vitro methods to analyze antimicrobial activity could play a role in the development of resistance to antibiotics. Current used methods analyze planktonic cells but for the method to be clinically relevant, biofilm in in vivo like conditions ought to be studied. Hence, our group has developed a qualitative and quantitative method with in vivo like 3D tissue for prediction of antimicrobial activity in reality. Devices (wound dressings) were applied on top of Pseudomonas aeruginosa inoculated Muller-Hinton (MH) agar or 3D synthetic soft tissues (SST) and incubated for 24 hours. The antibacterial activity was then analyzed visually and by viable counts. On MH agar two out of three silver containing devices showed zone of inhibitions (ZOI) and on SST, ZOI were detected for all three. Corroborating results were found upon evaluating the bacterial load in SST and shown to be silver concentration dependent. In conclusion, a novel method was developed combining visual rapid screening and quantitative evaluation of the antimicrobial activity in both tissue and devices. It uses tissue allowing biofilm formation thus mimicking reality closely. These conditions are essential in order to predict antimicrobial activity of medical devices in the task to prevent device related infections.http://dx.doi.org/10.1155/2014/768136 |
| spellingShingle | Bodil Hakonen Linnea K. Lönnberg Eva Larkö Kristina Blom A Novel Qualitative and Quantitative Biofilm Assay Based on 3D Soft Tissue International Journal of Biomaterials |
| title | A Novel Qualitative and Quantitative Biofilm Assay Based on 3D Soft Tissue |
| title_full | A Novel Qualitative and Quantitative Biofilm Assay Based on 3D Soft Tissue |
| title_fullStr | A Novel Qualitative and Quantitative Biofilm Assay Based on 3D Soft Tissue |
| title_full_unstemmed | A Novel Qualitative and Quantitative Biofilm Assay Based on 3D Soft Tissue |
| title_short | A Novel Qualitative and Quantitative Biofilm Assay Based on 3D Soft Tissue |
| title_sort | novel qualitative and quantitative biofilm assay based on 3d soft tissue |
| url | http://dx.doi.org/10.1155/2014/768136 |
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