Characteristics and Mechanism of Ammonia Nitrogen Removal by Heterotrophic Nitrification Bacterium <i>Klebsiella pneumoniae</i> LCU1 and Its Application in Wastewater Treatment
In this study, a novel strain exhibiting heterotrophic nitrification was screened; subsequently, the strain was identified as <i>Klebsiella pneumoniae</i> LCU1 using 16S rRNA gene sequencing. The aim of the study was to investigate the effects of external factors on the NH<sub>4<...
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2025-01-01
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| author | Meng Xu Lifei Chen Yizhen Xin Xiangyu Wang Zhuoya Wang Xueqiang Meng Wenyu Zhang Haoyang Sun Yifan Li Wenzhe Zhang Peng Wan Bingshuai Geng Lusheng Li |
| author_facet | Meng Xu Lifei Chen Yizhen Xin Xiangyu Wang Zhuoya Wang Xueqiang Meng Wenyu Zhang Haoyang Sun Yifan Li Wenzhe Zhang Peng Wan Bingshuai Geng Lusheng Li |
| author_sort | Meng Xu |
| collection | DOAJ |
| description | In this study, a novel strain exhibiting heterotrophic nitrification was screened; subsequently, the strain was identified as <i>Klebsiella pneumoniae</i> LCU1 using 16S rRNA gene sequencing. The aim of the study was to investigate the effects of external factors on the NH<sub>4</sub><sup>+</sup>-N removal efficiency of strain LCU1 in order to elucidate the optimal conditions for NH<sub>4</sub><sup>+</sup>-N removal by the strain and improve the removal efficiency. The findings indicated that the NH<sub>4</sub><sup>+</sup>-N removal efficiency of the strain exceeded 80% under optimal conditions (sodium succinate carbon source, C/N ratio of 10, initial pH of 8.0, temperature of 30 °C, and speed of 180 rpm). The genome analysis of strain LCU1 showed that key genes involved in nitrogen metabolism, including <i>nar</i>GHI, <i>nir</i>B, <i>nxr</i>AB, and <i>nas</i>AB, were successfully annotated; <i>hao</i> and <i>amo</i> were absent, but the nitrogen properties analysis determined that the strain had a heterotrophic nitrification ability. After 120 h, the NH<sub>4</sub><sup>+</sup>-N removal efficiency of strain LCU1 was 34.5% at a high NH<sub>4</sub><sup>+</sup>-N concentration of 2000 mg/L. More importantly, the NH<sub>4</sub><sup>+</sup>-N removal efficiency of this strain was above 34.13% at higher Cu<sup>2+</sup>, Mn<sup>2+</sup>, and Zn<sup>2+</sup> ion concentrations. Furthermore, strain LCU1 had the highest NH<sub>4</sub><sup>+</sup>-N removal efficiency of 34.51% for unsterilised (LCU1-OC) aquaculture wastewater. This suggests that with intensive colonisation treatment, the strain has promising application potential in real wastewater treatment. |
| format | Article |
| id | doaj-art-03f0be6a2f8b4895bf8bdb3ab6d696e4 |
| institution | DOAJ |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Microorganisms |
| spelling | doaj-art-03f0be6a2f8b4895bf8bdb3ab6d696e42025-08-20T02:45:00ZengMDPI AGMicroorganisms2076-26072025-01-0113229710.3390/microorganisms13020297Characteristics and Mechanism of Ammonia Nitrogen Removal by Heterotrophic Nitrification Bacterium <i>Klebsiella pneumoniae</i> LCU1 and Its Application in Wastewater TreatmentMeng Xu0Lifei Chen1Yizhen Xin2Xiangyu Wang3Zhuoya Wang4Xueqiang Meng5Wenyu Zhang6Haoyang Sun7Yifan Li8Wenzhe Zhang9Peng Wan10Bingshuai Geng11Lusheng Li12School of Agricultural Science and Biology, Liaocheng University, Liaocheng 252000, ChinaSchool of Agricultural Science and Biology, Liaocheng University, Liaocheng 252000, ChinaSchool of Agricultural Science and Biology, Liaocheng University, Liaocheng 252000, ChinaSchool of Agricultural Science and Biology, Liaocheng University, Liaocheng 252000, ChinaSchool of Agricultural Science and Biology, Liaocheng University, Liaocheng 252000, ChinaSchool of Agricultural Science and Biology, Liaocheng University, Liaocheng 252000, ChinaSchool of Agricultural Science and Biology, Liaocheng University, Liaocheng 252000, ChinaSchool of Agricultural Science and Biology, Liaocheng University, Liaocheng 252000, ChinaSchool of Agricultural Science and Biology, Liaocheng University, Liaocheng 252000, ChinaSchool of Agricultural Science and Biology, Liaocheng University, Liaocheng 252000, ChinaSchool of Agricultural Science and Biology, Liaocheng University, Liaocheng 252000, ChinaSchool of Agricultural Science and Biology, Liaocheng University, Liaocheng 252000, ChinaSchool of Agricultural Science and Biology, Liaocheng University, Liaocheng 252000, ChinaIn this study, a novel strain exhibiting heterotrophic nitrification was screened; subsequently, the strain was identified as <i>Klebsiella pneumoniae</i> LCU1 using 16S rRNA gene sequencing. The aim of the study was to investigate the effects of external factors on the NH<sub>4</sub><sup>+</sup>-N removal efficiency of strain LCU1 in order to elucidate the optimal conditions for NH<sub>4</sub><sup>+</sup>-N removal by the strain and improve the removal efficiency. The findings indicated that the NH<sub>4</sub><sup>+</sup>-N removal efficiency of the strain exceeded 80% under optimal conditions (sodium succinate carbon source, C/N ratio of 10, initial pH of 8.0, temperature of 30 °C, and speed of 180 rpm). The genome analysis of strain LCU1 showed that key genes involved in nitrogen metabolism, including <i>nar</i>GHI, <i>nir</i>B, <i>nxr</i>AB, and <i>nas</i>AB, were successfully annotated; <i>hao</i> and <i>amo</i> were absent, but the nitrogen properties analysis determined that the strain had a heterotrophic nitrification ability. After 120 h, the NH<sub>4</sub><sup>+</sup>-N removal efficiency of strain LCU1 was 34.5% at a high NH<sub>4</sub><sup>+</sup>-N concentration of 2000 mg/L. More importantly, the NH<sub>4</sub><sup>+</sup>-N removal efficiency of this strain was above 34.13% at higher Cu<sup>2+</sup>, Mn<sup>2+</sup>, and Zn<sup>2+</sup> ion concentrations. Furthermore, strain LCU1 had the highest NH<sub>4</sub><sup>+</sup>-N removal efficiency of 34.51% for unsterilised (LCU1-OC) aquaculture wastewater. This suggests that with intensive colonisation treatment, the strain has promising application potential in real wastewater treatment.https://www.mdpi.com/2076-2607/13/2/297<i>Klebsiella pneumoniae</i>heterotrophic nitrificationwastewater treatment |
| spellingShingle | Meng Xu Lifei Chen Yizhen Xin Xiangyu Wang Zhuoya Wang Xueqiang Meng Wenyu Zhang Haoyang Sun Yifan Li Wenzhe Zhang Peng Wan Bingshuai Geng Lusheng Li Characteristics and Mechanism of Ammonia Nitrogen Removal by Heterotrophic Nitrification Bacterium <i>Klebsiella pneumoniae</i> LCU1 and Its Application in Wastewater Treatment Microorganisms <i>Klebsiella pneumoniae</i> heterotrophic nitrification wastewater treatment |
| title | Characteristics and Mechanism of Ammonia Nitrogen Removal by Heterotrophic Nitrification Bacterium <i>Klebsiella pneumoniae</i> LCU1 and Its Application in Wastewater Treatment |
| title_full | Characteristics and Mechanism of Ammonia Nitrogen Removal by Heterotrophic Nitrification Bacterium <i>Klebsiella pneumoniae</i> LCU1 and Its Application in Wastewater Treatment |
| title_fullStr | Characteristics and Mechanism of Ammonia Nitrogen Removal by Heterotrophic Nitrification Bacterium <i>Klebsiella pneumoniae</i> LCU1 and Its Application in Wastewater Treatment |
| title_full_unstemmed | Characteristics and Mechanism of Ammonia Nitrogen Removal by Heterotrophic Nitrification Bacterium <i>Klebsiella pneumoniae</i> LCU1 and Its Application in Wastewater Treatment |
| title_short | Characteristics and Mechanism of Ammonia Nitrogen Removal by Heterotrophic Nitrification Bacterium <i>Klebsiella pneumoniae</i> LCU1 and Its Application in Wastewater Treatment |
| title_sort | characteristics and mechanism of ammonia nitrogen removal by heterotrophic nitrification bacterium i klebsiella pneumoniae i lcu1 and its application in wastewater treatment |
| topic | <i>Klebsiella pneumoniae</i> heterotrophic nitrification wastewater treatment |
| url | https://www.mdpi.com/2076-2607/13/2/297 |
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