Molecular and epidemiologyical analysis of a Campylobacter jejuni outbreak in China, 2018
Introduction: Campylobacter spp. is the most common gastrointestinal pathogen worldwide with a very low reported incidence in China. In April 2018, one 36 cases of diarrhea outbreak occurred in a high school in Beijing after a trip to another province in Southern China. The investigation for the en...
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The Journal of Infection in Developing Countries
2019-12-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/11408 |
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| author | Mei Qu Maojun Zhang Xin Zhang Lei Jia Jun Xu Yanhui Chu Zhichao Liang Bing Lv Hao Liang Ying Huang Quanyi Wang |
| author_facet | Mei Qu Maojun Zhang Xin Zhang Lei Jia Jun Xu Yanhui Chu Zhichao Liang Bing Lv Hao Liang Ying Huang Quanyi Wang |
| author_sort | Mei Qu |
| collection | DOAJ |
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Introduction: Campylobacter spp. is the most common gastrointestinal pathogen worldwide with a very low reported incidence in China. In April 2018, one 36 cases of diarrhea outbreak occurred in a high school in Beijing after a trip to another province in Southern China. The investigation for the enteric pathogen infection was conducted to identify the cause.
Methodology: Eighteen stool specimens from 11 diarrheal patients and 3 close contacts were collected and tested for 16 enteric bacterial and viral pathogens using real-time PCR methods. Multilocus Sequence Typing (MLST), pulsed-field gel electrophoresis (PFGE) and antimicrobial susceptibility testing were applied to C. jejuni isolates in this outbreak in order to characterize the incident.
Results: Ten (90.9%) of 11 stool specimens from diarrheal patients were positive for Campylobacter jejuni, confirmed as the cause of outbreak. Eight C. jejuni strains were obtained and produced 2 sequence types (STs) and 3 PFGE patterns. Six of them had the same ST (ST2274) and PFGE pattern (SMA001). The dominant outbreak strain with an identical subtyping profile was clustered with most chicken isolates from national MLST and PulseNet Campylobacter database. The entire 8 isolates were multi-drug resistant, with the dominant resistance pattern of nalidixic acid, tetracycline and ciprofloxacin combined, except for 2 isolates resistant to florfenicol.
Conclusion: Molecular typing confirmed that most of the cases belonged to a clonal cluster supporting the hypothesis of a common source; however, the source was not identified. This was the first recognized Campylobacteriosis outbreak in China among 20 years.
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| format | Article |
| id | doaj-art-03eeb7e84bfe4f069e4a0e57baaaf804 |
| institution | DOAJ |
| issn | 1972-2680 |
| language | English |
| publishDate | 2019-12-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-03eeb7e84bfe4f069e4a0e57baaaf8042025-08-20T02:57:01ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802019-12-01131210.3855/jidc.11408Molecular and epidemiologyical analysis of a Campylobacter jejuni outbreak in China, 2018Mei Qu0Maojun Zhang1Xin Zhang2Lei Jia3Jun Xu4Yanhui Chu5Zhichao Liang6Bing Lv7Hao Liang8Ying Huang9Quanyi Wang10Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Institute of Infectious and Endemic Diseases Control, Beijing Center for Disease Prevention and Control, Beijing Research Center for Preventive Medicine, Beijing, 100013, ChinaState Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Changping, Beijing, 102206, ChinaBeijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Institute of Infectious and Endemic Diseases Control, Beijing Center for Disease Prevention and Control, Beijing Research Center for Preventive Medicine, Beijing, 100013, ChinaBeijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Institute of Infectious and Endemic Diseases Control, Beijing Center for Disease Prevention and Control, Beijing Research Center for Preventive Medicine, Beijing, 100013, ChinaBeijing Xicheng District Center for Diseases Control and Prevention, ChinaBeijing Xicheng District Center for Diseases Control and Prevention, ChinaBeijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Institute of Infectious and Endemic Diseases Control, Beijing Center for Disease Prevention and Control, Beijing Research Center for Preventive Medicine, Beijing, 100013, ChinaBeijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Institute of Infectious and Endemic Diseases Control, Beijing Center for Disease Prevention and Control, Beijing Research Center for Preventive Medicine, Beijing, 100013, ChinaState Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Changping, Beijing, 102206, ChinaBeijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Institute of Infectious and Endemic Diseases Control, Beijing Center for Disease Prevention and Control, Beijing Research Center for Preventive Medicine, Beijing, 100013, ChinaBeijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Institute of Infectious and Endemic Diseases Control, Beijing Center for Disease Prevention and Control, Beijing Research Center for Preventive Medicine, Beijing, 100013, China Introduction: Campylobacter spp. is the most common gastrointestinal pathogen worldwide with a very low reported incidence in China. In April 2018, one 36 cases of diarrhea outbreak occurred in a high school in Beijing after a trip to another province in Southern China. The investigation for the enteric pathogen infection was conducted to identify the cause. Methodology: Eighteen stool specimens from 11 diarrheal patients and 3 close contacts were collected and tested for 16 enteric bacterial and viral pathogens using real-time PCR methods. Multilocus Sequence Typing (MLST), pulsed-field gel electrophoresis (PFGE) and antimicrobial susceptibility testing were applied to C. jejuni isolates in this outbreak in order to characterize the incident. Results: Ten (90.9%) of 11 stool specimens from diarrheal patients were positive for Campylobacter jejuni, confirmed as the cause of outbreak. Eight C. jejuni strains were obtained and produced 2 sequence types (STs) and 3 PFGE patterns. Six of them had the same ST (ST2274) and PFGE pattern (SMA001). The dominant outbreak strain with an identical subtyping profile was clustered with most chicken isolates from national MLST and PulseNet Campylobacter database. The entire 8 isolates were multi-drug resistant, with the dominant resistance pattern of nalidixic acid, tetracycline and ciprofloxacin combined, except for 2 isolates resistant to florfenicol. Conclusion: Molecular typing confirmed that most of the cases belonged to a clonal cluster supporting the hypothesis of a common source; however, the source was not identified. This was the first recognized Campylobacteriosis outbreak in China among 20 years. https://jidc.org/index.php/journal/article/view/11408OutbreakCampylobacter jejuniDiarrheaMolecular subtyping |
| spellingShingle | Mei Qu Maojun Zhang Xin Zhang Lei Jia Jun Xu Yanhui Chu Zhichao Liang Bing Lv Hao Liang Ying Huang Quanyi Wang Molecular and epidemiologyical analysis of a Campylobacter jejuni outbreak in China, 2018 Journal of Infection in Developing Countries Outbreak Campylobacter jejuni Diarrhea Molecular subtyping |
| title | Molecular and epidemiologyical analysis of a Campylobacter jejuni outbreak in China, 2018 |
| title_full | Molecular and epidemiologyical analysis of a Campylobacter jejuni outbreak in China, 2018 |
| title_fullStr | Molecular and epidemiologyical analysis of a Campylobacter jejuni outbreak in China, 2018 |
| title_full_unstemmed | Molecular and epidemiologyical analysis of a Campylobacter jejuni outbreak in China, 2018 |
| title_short | Molecular and epidemiologyical analysis of a Campylobacter jejuni outbreak in China, 2018 |
| title_sort | molecular and epidemiologyical analysis of a campylobacter jejuni outbreak in china 2018 |
| topic | Outbreak Campylobacter jejuni Diarrhea Molecular subtyping |
| url | https://jidc.org/index.php/journal/article/view/11408 |
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