Molecular detection, seasonality, epidemiology and effect of Brucella melitensis infection on the hematological profile of cattle breeds
Abstract Brucellosis is a bacterial zoonosis caused by various Brucella species, affecting both animals and humans. The incidence is particularly high in tropical and subtropical regions that are known for higher tick density. Present study was designed to improve our knowledge regarding Brucella me...
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Nature Portfolio
2025-04-01
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| author | Babar Ali Muhammad Naeem Shakir Ullah Hamza Manzoor Muhammad Asif Nasir Mahmood Aliza Kanwal Mohammed Bourhia Musaab Dauelbait Turki M. Dawoud Adil Khan Ahmad Ali Furhan Iqbal |
| author_facet | Babar Ali Muhammad Naeem Shakir Ullah Hamza Manzoor Muhammad Asif Nasir Mahmood Aliza Kanwal Mohammed Bourhia Musaab Dauelbait Turki M. Dawoud Adil Khan Ahmad Ali Furhan Iqbal |
| author_sort | Babar Ali |
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| description | Abstract Brucellosis is a bacterial zoonosis caused by various Brucella species, affecting both animals and humans. The incidence is particularly high in tropical and subtropical regions that are known for higher tick density. Present study was designed to improve our knowledge regarding Brucella melitensis infection in Pakistani cattle. A total of 903 blood samples were collected. Three cattle breeds, Holstein Friesian (N = 298), Sahiwal (N = 299), and crossbred (N = 306), were enrolled along with epidemiological data during March 2022 till April 2023 from district Multan in Punjab province. A PCR-based approach targeting IS711 estimated an overall prevalence of 4% (39/903). Representative partial IS711 sequences of three Pakistani isolates revealed a single genotype that was similar to Brucella melitensis sequences reported from aborted fetus in China. All cattle breeds were equally susceptible to this infection. For Sahiwal breed, bacterial infection varied between sampling seasons. Epidemiological factor analysis revealed that farms where only cattle were reared and dogs at farm having tick load had higher Brucella melitensis infection rates. Red blood cells, lymphocyte (%), monocyte (%), mean cell volume, hematocrit, mean corpuscular hemoglobin concentration and platelet count were significantly disturbed in Brucella melitensis positive cattle of all three breeds. A longitudinal survey and phylogenetic positioning of Brucella melitensis is recommended for epidemiological correlation, diagnosis and treatment of brucellosis in Pakistan. |
| format | Article |
| id | doaj-art-03ea7f44094943eb881640719ea68193 |
| institution | OA Journals |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-03ea7f44094943eb881640719ea681932025-08-20T02:17:01ZengNature PortfolioScientific Reports2045-23222025-04-0115111110.1038/s41598-025-94370-xMolecular detection, seasonality, epidemiology and effect of Brucella melitensis infection on the hematological profile of cattle breedsBabar Ali0Muhammad Naeem1Shakir Ullah2Hamza Manzoor3Muhammad Asif4Nasir Mahmood5Aliza Kanwal6Mohammed Bourhia7Musaab Dauelbait8Turki M. Dawoud9Adil Khan10Ahmad Ali11Furhan Iqbal12Department of Zoology, Islamia UniversityInstitute of Zoology, Bahauddin Zakariya UniversityDepartment of Zoology, Abdul Wali Khan UniversityDepartment of Theriogenology, University of Veterinary and Animal Sciences LahoreInstitute of Zoology, Bahauddin Zakariya UniversityFaculty of Veterinary Sciences, Bahauddin Zakariya University MultanInstitute of Zoology, Bahauddin Zakariya UniversitySwalife Biotech Ltd Unit 3D North Point HouseUniversity of Bahr el GhazalDepartment of Botany and Microbiology, College of Science, King Saud UniversityDepartment of Botany and Zoology, Bacha Khan UniversityDepartment of Zoology, Islamia UniversityInstitute of Zoology, Bahauddin Zakariya UniversityAbstract Brucellosis is a bacterial zoonosis caused by various Brucella species, affecting both animals and humans. The incidence is particularly high in tropical and subtropical regions that are known for higher tick density. Present study was designed to improve our knowledge regarding Brucella melitensis infection in Pakistani cattle. A total of 903 blood samples were collected. Three cattle breeds, Holstein Friesian (N = 298), Sahiwal (N = 299), and crossbred (N = 306), were enrolled along with epidemiological data during March 2022 till April 2023 from district Multan in Punjab province. A PCR-based approach targeting IS711 estimated an overall prevalence of 4% (39/903). Representative partial IS711 sequences of three Pakistani isolates revealed a single genotype that was similar to Brucella melitensis sequences reported from aborted fetus in China. All cattle breeds were equally susceptible to this infection. For Sahiwal breed, bacterial infection varied between sampling seasons. Epidemiological factor analysis revealed that farms where only cattle were reared and dogs at farm having tick load had higher Brucella melitensis infection rates. Red blood cells, lymphocyte (%), monocyte (%), mean cell volume, hematocrit, mean corpuscular hemoglobin concentration and platelet count were significantly disturbed in Brucella melitensis positive cattle of all three breeds. A longitudinal survey and phylogenetic positioning of Brucella melitensis is recommended for epidemiological correlation, diagnosis and treatment of brucellosis in Pakistan.https://doi.org/10.1038/s41598-025-94370-xBrucella melitensisMolecular prevalenceRisk factorsPhylogenetic analysisCattle breeds |
| spellingShingle | Babar Ali Muhammad Naeem Shakir Ullah Hamza Manzoor Muhammad Asif Nasir Mahmood Aliza Kanwal Mohammed Bourhia Musaab Dauelbait Turki M. Dawoud Adil Khan Ahmad Ali Furhan Iqbal Molecular detection, seasonality, epidemiology and effect of Brucella melitensis infection on the hematological profile of cattle breeds Scientific Reports Brucella melitensis Molecular prevalence Risk factors Phylogenetic analysis Cattle breeds |
| title | Molecular detection, seasonality, epidemiology and effect of Brucella melitensis infection on the hematological profile of cattle breeds |
| title_full | Molecular detection, seasonality, epidemiology and effect of Brucella melitensis infection on the hematological profile of cattle breeds |
| title_fullStr | Molecular detection, seasonality, epidemiology and effect of Brucella melitensis infection on the hematological profile of cattle breeds |
| title_full_unstemmed | Molecular detection, seasonality, epidemiology and effect of Brucella melitensis infection on the hematological profile of cattle breeds |
| title_short | Molecular detection, seasonality, epidemiology and effect of Brucella melitensis infection on the hematological profile of cattle breeds |
| title_sort | molecular detection seasonality epidemiology and effect of brucella melitensis infection on the hematological profile of cattle breeds |
| topic | Brucella melitensis Molecular prevalence Risk factors Phylogenetic analysis Cattle breeds |
| url | https://doi.org/10.1038/s41598-025-94370-x |
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