Deterrent Effects of Clary Sage Oil and Two Major Constituents against <em>Drosophila suzukii</em> (Diptera: Drosophilidae)
<i>Drosophila suzukii</i> (Diptera: Drosophilidae), spotted-wing drosophila, poses a significant threat to soft-skinned fruit crops in the Americas, Europe, Africa, and Oceania, as well as in Asia. The application of chemical insecticides is the primary control strategy for <i>D. s...
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2024-09-01
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| author | Yu Wang Fengyi Wen Xiangyi Zhou Guoxing Chen Chunxia Tian Jiali Qian Huiming Wu Mengli Chen |
| author_facet | Yu Wang Fengyi Wen Xiangyi Zhou Guoxing Chen Chunxia Tian Jiali Qian Huiming Wu Mengli Chen |
| author_sort | Yu Wang |
| collection | DOAJ |
| description | <i>Drosophila suzukii</i> (Diptera: Drosophilidae), spotted-wing drosophila, poses a significant threat to soft-skinned fruit crops in the Americas, Europe, Africa, and Oceania, as well as in Asia. The application of chemical insecticides is the primary control strategy for <i>D. suzukii</i>; however, resistance has developed with the indiscriminate use of chemical insecticides. Essential oils, considered potential alternatives to pesticidal strategies, exhibit potent toxic and sublethal behavioral effects against numerous pests, including <i>D. suzukii</i>. Clary sage oil repels a variety of agricultural and household pests; however, whether it has a repellent effect against <i>D. suzukii</i> remains unknown. Here, we found that clary sage oil exhibited dose-dependent repellency against <i>D. suzukii</i> adults in a T-maze assay, a two-choice assay and a two-choice attraction assay. Also, clary sage oil showed a significant repellent effect against <i>D. suzukii</i> larvae. Next, we explored the chemical constituents of clary sage oil by GC-MS and identified two major constituents, linalyl acetate (40.03%) and linalool (23.02%). Furthermore, the behavioral assays of linalyl acetate and linalool showed that both compounds conferred comparable repellency against <i>D. suzukii</i> adults and larvae. Finally, we found clary sage oil, linalyl acetate, and linalool elicited EAG responses in <i>D. suzukii</i>, especially clary sage oil, suggesting the repellency was mediated by the olfactory system. These findings indicate that <i>D. suzukii</i> shows olfactory-based behavioral avoidance of clary sage oil, linalyl acetate, and linalool. Clary sage oil and its major constituents may be possible alternatives in the management of <i>D. suzukii</i>. |
| format | Article |
| id | doaj-art-03e61715495143559096bd44e82b98e3 |
| institution | Kabale University |
| issn | 2075-4450 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj-art-03e61715495143559096bd44e82b98e32025-01-09T16:06:31ZengMDPI AGInsects2075-44502024-09-01151073310.3390/insects15100733Deterrent Effects of Clary Sage Oil and Two Major Constituents against <em>Drosophila suzukii</em> (Diptera: Drosophilidae)Yu Wang0Fengyi Wen1Xiangyi Zhou2Guoxing Chen3Chunxia Tian4Jiali Qian5Huiming Wu6Mengli Chen7Zhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, College of Advanced Agricultural Sciences, Zhejiang A&F University, Hangzhou 311300, ChinaZhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, College of Advanced Agricultural Sciences, Zhejiang A&F University, Hangzhou 311300, ChinaZhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, College of Advanced Agricultural Sciences, Zhejiang A&F University, Hangzhou 311300, ChinaZhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, College of Advanced Agricultural Sciences, Zhejiang A&F University, Hangzhou 311300, ChinaZhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, ChinaInstitute of Pesticide and Environmental Toxicology, Zhejiang University, Hangzhou 310030, ChinaZhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, College of Advanced Agricultural Sciences, Zhejiang A&F University, Hangzhou 311300, ChinaZhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, College of Advanced Agricultural Sciences, Zhejiang A&F University, Hangzhou 311300, China<i>Drosophila suzukii</i> (Diptera: Drosophilidae), spotted-wing drosophila, poses a significant threat to soft-skinned fruit crops in the Americas, Europe, Africa, and Oceania, as well as in Asia. The application of chemical insecticides is the primary control strategy for <i>D. suzukii</i>; however, resistance has developed with the indiscriminate use of chemical insecticides. Essential oils, considered potential alternatives to pesticidal strategies, exhibit potent toxic and sublethal behavioral effects against numerous pests, including <i>D. suzukii</i>. Clary sage oil repels a variety of agricultural and household pests; however, whether it has a repellent effect against <i>D. suzukii</i> remains unknown. Here, we found that clary sage oil exhibited dose-dependent repellency against <i>D. suzukii</i> adults in a T-maze assay, a two-choice assay and a two-choice attraction assay. Also, clary sage oil showed a significant repellent effect against <i>D. suzukii</i> larvae. Next, we explored the chemical constituents of clary sage oil by GC-MS and identified two major constituents, linalyl acetate (40.03%) and linalool (23.02%). Furthermore, the behavioral assays of linalyl acetate and linalool showed that both compounds conferred comparable repellency against <i>D. suzukii</i> adults and larvae. Finally, we found clary sage oil, linalyl acetate, and linalool elicited EAG responses in <i>D. suzukii</i>, especially clary sage oil, suggesting the repellency was mediated by the olfactory system. These findings indicate that <i>D. suzukii</i> shows olfactory-based behavioral avoidance of clary sage oil, linalyl acetate, and linalool. Clary sage oil and its major constituents may be possible alternatives in the management of <i>D. suzukii</i>.https://www.mdpi.com/2075-4450/15/10/733spotted-wing drosophilaclary sage oillinalyl acetatelinaloolrepellencyEAG |
| spellingShingle | Yu Wang Fengyi Wen Xiangyi Zhou Guoxing Chen Chunxia Tian Jiali Qian Huiming Wu Mengli Chen Deterrent Effects of Clary Sage Oil and Two Major Constituents against <em>Drosophila suzukii</em> (Diptera: Drosophilidae) Insects spotted-wing drosophila clary sage oil linalyl acetate linalool repellency EAG |
| title | Deterrent Effects of Clary Sage Oil and Two Major Constituents against <em>Drosophila suzukii</em> (Diptera: Drosophilidae) |
| title_full | Deterrent Effects of Clary Sage Oil and Two Major Constituents against <em>Drosophila suzukii</em> (Diptera: Drosophilidae) |
| title_fullStr | Deterrent Effects of Clary Sage Oil and Two Major Constituents against <em>Drosophila suzukii</em> (Diptera: Drosophilidae) |
| title_full_unstemmed | Deterrent Effects of Clary Sage Oil and Two Major Constituents against <em>Drosophila suzukii</em> (Diptera: Drosophilidae) |
| title_short | Deterrent Effects of Clary Sage Oil and Two Major Constituents against <em>Drosophila suzukii</em> (Diptera: Drosophilidae) |
| title_sort | deterrent effects of clary sage oil and two major constituents against em drosophila suzukii em diptera drosophilidae |
| topic | spotted-wing drosophila clary sage oil linalyl acetate linalool repellency EAG |
| url | https://www.mdpi.com/2075-4450/15/10/733 |
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