Repeated harvest enables efficient production of VSV-GP
Viral products keep gaining importance in multiple therapeutic fields. Considering the scale and production slot limitations, optimizing the outcome of every manufacturing batch is essential to minimize costs and make this therapeutic modality broadly available to patients. Most manufacturing proces...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-12-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2024.1505338/full |
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| author | Rebecca Habisch Peter Neubauer Jorge Soza-Ried Eva Puschmann |
| author_facet | Rebecca Habisch Peter Neubauer Jorge Soza-Ried Eva Puschmann |
| author_sort | Rebecca Habisch |
| collection | DOAJ |
| description | Viral products keep gaining importance in multiple therapeutic fields. Considering the scale and production slot limitations, optimizing the outcome of every manufacturing batch is essential to minimize costs and make this therapeutic modality broadly available to patients. Most manufacturing processes for oncolytic viruses currently in clinical studies are based on a batch process. Here, we evaluated the benefits in terms of titer increase of a repeated harvest approach and compared it to the classical batch production process. While no effect on cell density was observed, the cumulated infectious titer following repeated harvest was over 400 times higher than the evaluated batch process yield. This shows that repeated harvests or perfusion have the potential to boost viral yields and should be considered when deciding on a process format for production. |
| format | Article |
| id | doaj-art-03d3e90ad5e5409ca7e7051db8fc296a |
| institution | Kabale University |
| issn | 2296-4185 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj-art-03d3e90ad5e5409ca7e7051db8fc296a2024-12-05T10:40:25ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852024-12-011210.3389/fbioe.2024.15053381505338Repeated harvest enables efficient production of VSV-GPRebecca Habisch0Peter Neubauer1Jorge Soza-Ried2Eva Puschmann3Boehringer Ingelheim, Viral Therapeutics Center, Ochsenhausen, GermanyDepartment of Bioprocess Engineering, Institute of Biotechnology, Technische Universität Berlin, Berlin, GermanyBoehringer Ingelheim, Viral Therapeutics Center, Ochsenhausen, GermanyBoehringer Ingelheim, Viral Therapeutics Center, Ochsenhausen, GermanyViral products keep gaining importance in multiple therapeutic fields. Considering the scale and production slot limitations, optimizing the outcome of every manufacturing batch is essential to minimize costs and make this therapeutic modality broadly available to patients. Most manufacturing processes for oncolytic viruses currently in clinical studies are based on a batch process. Here, we evaluated the benefits in terms of titer increase of a repeated harvest approach and compared it to the classical batch production process. While no effect on cell density was observed, the cumulated infectious titer following repeated harvest was over 400 times higher than the evaluated batch process yield. This shows that repeated harvests or perfusion have the potential to boost viral yields and should be considered when deciding on a process format for production.https://www.frontiersin.org/articles/10.3389/fbioe.2024.1505338/fullVSV-GPoncolytic virus productionbioprocess developmentrepeated-batchperfusion |
| spellingShingle | Rebecca Habisch Peter Neubauer Jorge Soza-Ried Eva Puschmann Repeated harvest enables efficient production of VSV-GP Frontiers in Bioengineering and Biotechnology VSV-GP oncolytic virus production bioprocess development repeated-batch perfusion |
| title | Repeated harvest enables efficient production of VSV-GP |
| title_full | Repeated harvest enables efficient production of VSV-GP |
| title_fullStr | Repeated harvest enables efficient production of VSV-GP |
| title_full_unstemmed | Repeated harvest enables efficient production of VSV-GP |
| title_short | Repeated harvest enables efficient production of VSV-GP |
| title_sort | repeated harvest enables efficient production of vsv gp |
| topic | VSV-GP oncolytic virus production bioprocess development repeated-batch perfusion |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2024.1505338/full |
| work_keys_str_mv | AT rebeccahabisch repeatedharvestenablesefficientproductionofvsvgp AT peterneubauer repeatedharvestenablesefficientproductionofvsvgp AT jorgesozaried repeatedharvestenablesefficientproductionofvsvgp AT evapuschmann repeatedharvestenablesefficientproductionofvsvgp |