Contribution of Common Sulfur-Containing Substrates to Hydrogen Sulfide Production By Human Gut Microbiota Using an In Vitro Model Standardized For Bacterial Counts
Hydrogen sulfide (H2S) produced by human gut microbiota is highly toxic and implicated in pathogenesis of gastrointestinal tract disorders. Sulfur-containing amino acid (SAA) degradation is a major contributor to its production, but SAA degradation pathways have not been extensively characterized. I...
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Taylor & Francis Group
2024-12-01
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| Series: | Gut Microbes Reports |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/29933935.2024.2361246 |
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| author | Levi M. Teigen Thomas Kaiser-Powers Michael Matson Baila Elkin Amanda J. Kabage Matthew Hamilton Byron P Vaughn Michael J. Sadowsky Christopher Staley Alexander Khoruts |
| author_facet | Levi M. Teigen Thomas Kaiser-Powers Michael Matson Baila Elkin Amanda J. Kabage Matthew Hamilton Byron P Vaughn Michael J. Sadowsky Christopher Staley Alexander Khoruts |
| author_sort | Levi M. Teigen |
| collection | DOAJ |
| description | Hydrogen sulfide (H2S) produced by human gut microbiota is highly toxic and implicated in pathogenesis of gastrointestinal tract disorders. Sulfur-containing amino acid (SAA) degradation is a major contributor to its production, but SAA degradation pathways have not been extensively characterized. In vitro model systems of fecal H2S production offer a basic method to help elucidate SAA degradation pathways, but the approach is not standardized. To address this, we used fecal microbiota separated from feces and standardized for bacterial counts to measure H2S production potential in response to different substrates in healthy controls (n = 6) with repeated sampling (three samples per participant). H2S production was highest with cysteine (mean = 16.7 ppm) compared to sodium sulfate (0.7 ppm) and taurine (0.8 ppm). Sodium-sulfate-driven H2S production negatively correlated with Ruminococcus (Spearman’s ρ = −0.5) and cysteine-driven H2S production negatively correlated with Firmicutes (Spearman’s ρ = −0.5). These findings, using a protocol controlling for confounding variables such as bacterial counts, validate previous findings of cysteine as a primary driver of H2S production. Finally, the inclusion of samples from two patients with UC allowed for the illustration of the potential of this approach to identify functional differences in specific disease states. |
| format | Article |
| id | doaj-art-03b9da18efa9417eb7af02675eef0e86 |
| institution | DOAJ |
| issn | 2993-3935 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Gut Microbes Reports |
| spelling | doaj-art-03b9da18efa9417eb7af02675eef0e862025-08-20T03:10:37ZengTaylor & Francis GroupGut Microbes Reports2993-39352024-12-011110.1080/29933935.2024.2361246Contribution of Common Sulfur-Containing Substrates to Hydrogen Sulfide Production By Human Gut Microbiota Using an In Vitro Model Standardized For Bacterial CountsLevi M. Teigen0Thomas Kaiser-Powers1Michael Matson2Baila Elkin3Amanda J. Kabage4Matthew Hamilton5Byron P Vaughn6Michael J. Sadowsky7Christopher Staley8Alexander Khoruts9Department of Food Science and Nutrition, University of Minnesota, St. Paul, MN, USADivision of Basic and Translational Research, Department of Surgery, University of Minnesota, Minneapolis, MN, USADivision of Gastroenterology, Hepatology, and Nutrition, Department of Medicine, University of Minnesota, Minneapolis, MN, USAMedical School, University of Minnesota, Minneapolis, MN, USADivision of Gastroenterology, Hepatology, and Nutrition, Department of Medicine, University of Minnesota, Minneapolis, MN, USAMicrobiota Therapeutics Program, University of Minnesota, Minneapolis, MN, USADivision of Gastroenterology, Hepatology, and Nutrition, Department of Medicine, University of Minnesota, Minneapolis, MN, USADepartment of Soil, Water, and Climate and Department of Plant and Microbial Biology, University of Minnesota, Minneapolis, MN, USADivision of Basic and Translational Research, Department of Surgery, University of Minnesota, Minneapolis, MN, USADivision of Gastroenterology, Hepatology, and Nutrition, Department of Medicine, University of Minnesota, Minneapolis, MN, USAHydrogen sulfide (H2S) produced by human gut microbiota is highly toxic and implicated in pathogenesis of gastrointestinal tract disorders. Sulfur-containing amino acid (SAA) degradation is a major contributor to its production, but SAA degradation pathways have not been extensively characterized. In vitro model systems of fecal H2S production offer a basic method to help elucidate SAA degradation pathways, but the approach is not standardized. To address this, we used fecal microbiota separated from feces and standardized for bacterial counts to measure H2S production potential in response to different substrates in healthy controls (n = 6) with repeated sampling (three samples per participant). H2S production was highest with cysteine (mean = 16.7 ppm) compared to sodium sulfate (0.7 ppm) and taurine (0.8 ppm). Sodium-sulfate-driven H2S production negatively correlated with Ruminococcus (Spearman’s ρ = −0.5) and cysteine-driven H2S production negatively correlated with Firmicutes (Spearman’s ρ = −0.5). These findings, using a protocol controlling for confounding variables such as bacterial counts, validate previous findings of cysteine as a primary driver of H2S production. Finally, the inclusion of samples from two patients with UC allowed for the illustration of the potential of this approach to identify functional differences in specific disease states.https://www.tandfonline.com/doi/10.1080/29933935.2024.2361246Microbiomehydrogen sulfidecysteinesulfatetaurine |
| spellingShingle | Levi M. Teigen Thomas Kaiser-Powers Michael Matson Baila Elkin Amanda J. Kabage Matthew Hamilton Byron P Vaughn Michael J. Sadowsky Christopher Staley Alexander Khoruts Contribution of Common Sulfur-Containing Substrates to Hydrogen Sulfide Production By Human Gut Microbiota Using an In Vitro Model Standardized For Bacterial Counts Gut Microbes Reports Microbiome hydrogen sulfide cysteine sulfate taurine |
| title | Contribution of Common Sulfur-Containing Substrates to Hydrogen Sulfide Production By Human Gut Microbiota Using an In Vitro Model Standardized For Bacterial Counts |
| title_full | Contribution of Common Sulfur-Containing Substrates to Hydrogen Sulfide Production By Human Gut Microbiota Using an In Vitro Model Standardized For Bacterial Counts |
| title_fullStr | Contribution of Common Sulfur-Containing Substrates to Hydrogen Sulfide Production By Human Gut Microbiota Using an In Vitro Model Standardized For Bacterial Counts |
| title_full_unstemmed | Contribution of Common Sulfur-Containing Substrates to Hydrogen Sulfide Production By Human Gut Microbiota Using an In Vitro Model Standardized For Bacterial Counts |
| title_short | Contribution of Common Sulfur-Containing Substrates to Hydrogen Sulfide Production By Human Gut Microbiota Using an In Vitro Model Standardized For Bacterial Counts |
| title_sort | contribution of common sulfur containing substrates to hydrogen sulfide production by human gut microbiota using an in vitro model standardized for bacterial counts |
| topic | Microbiome hydrogen sulfide cysteine sulfate taurine |
| url | https://www.tandfonline.com/doi/10.1080/29933935.2024.2361246 |
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