<i>Daphne kiusiana</i> Crude Extract and Its Fraction Enhance Keratinocyte Migration via the ERK/MMP9 Pathway
<i>Daphne kiusiana</i> is a naturally occurring plant in East Asia belonging to the Thymelaeaceae family. While its biological properties have been explored, its potential role in wound healing remains largely unknown. This study investigated the effects of <i>Daphne kiusiana</i...
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2025-04-01
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| author | Jinu Lee Seon Min Oh Hyung Won Ryu Jeong-Hwa Baek |
| author_facet | Jinu Lee Seon Min Oh Hyung Won Ryu Jeong-Hwa Baek |
| author_sort | Jinu Lee |
| collection | DOAJ |
| description | <i>Daphne kiusiana</i> is a naturally occurring plant in East Asia belonging to the Thymelaeaceae family. While its biological properties have been explored, its potential role in wound healing remains largely unknown. This study investigated the effects of <i>Daphne kiusiana</i> extracts on keratinocyte migration and the underlying mechanisms. The crude extract and its fractions was tested in vitro at various concentrations to evaluate their ability to promote keratinocyte migration, and a cytotoxicity assay was conducted to assess cell viability. The results demonstrated that <i>Daphne kiusiana</i> significantly enhanced keratinocyte migration without inducing notable cytotoxicity at tested concentrations. Mechanistically, this effect was mediated through the modulation of matrix metalloproteinase-9 (MMP-9) via extracellular signal-regulated kinase (ERK) signaling, which plays a crucial role in keratinocyte migration. These findings suggest that <i>Daphne kiusiana</i> may serve as a potential therapeutic agent for enhancing keratinocyte migration in wound healing. However, further investigations, including clinical studies, are necessary to confirm its efficacy and safety. To our knowledge, this is the first study to report the potential of <i>Daphne kiusiana</i> in promoting keratinocyte migration, offering new insights into wound healing therapies. |
| format | Article |
| id | doaj-art-03b35daef5a940608e13b375bece0240 |
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| issn | 1467-3037 1467-3045 |
| language | English |
| publishDate | 2025-04-01 |
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| series | Current Issues in Molecular Biology |
| spelling | doaj-art-03b35daef5a940608e13b375bece02402025-08-20T01:56:25ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452025-04-0147530010.3390/cimb47050300<i>Daphne kiusiana</i> Crude Extract and Its Fraction Enhance Keratinocyte Migration via the ERK/MMP9 PathwayJinu Lee0Seon Min Oh1Hyung Won Ryu2Jeong-Hwa Baek3Department of Molecular Genetics, School of Dentistry and Dental Research Institute, Seoul National University, Seoul 08826, Republic of KoreaNatural Product Research Center and Natural Product Central Bank, Korea Research Institute of Bioscience and Biotechnology, Cheongju-si 28116, Republic of KoreaNatural Product Research Center and Natural Product Central Bank, Korea Research Institute of Bioscience and Biotechnology, Cheongju-si 28116, Republic of KoreaDepartment of Molecular Genetics, School of Dentistry and Dental Research Institute, Seoul National University, Seoul 08826, Republic of Korea<i>Daphne kiusiana</i> is a naturally occurring plant in East Asia belonging to the Thymelaeaceae family. While its biological properties have been explored, its potential role in wound healing remains largely unknown. This study investigated the effects of <i>Daphne kiusiana</i> extracts on keratinocyte migration and the underlying mechanisms. The crude extract and its fractions was tested in vitro at various concentrations to evaluate their ability to promote keratinocyte migration, and a cytotoxicity assay was conducted to assess cell viability. The results demonstrated that <i>Daphne kiusiana</i> significantly enhanced keratinocyte migration without inducing notable cytotoxicity at tested concentrations. Mechanistically, this effect was mediated through the modulation of matrix metalloproteinase-9 (MMP-9) via extracellular signal-regulated kinase (ERK) signaling, which plays a crucial role in keratinocyte migration. These findings suggest that <i>Daphne kiusiana</i> may serve as a potential therapeutic agent for enhancing keratinocyte migration in wound healing. However, further investigations, including clinical studies, are necessary to confirm its efficacy and safety. To our knowledge, this is the first study to report the potential of <i>Daphne kiusiana</i> in promoting keratinocyte migration, offering new insights into wound healing therapies.https://www.mdpi.com/1467-3045/47/5/300<i>Daphne kiusiana</i>phytomedicinewound healingkeratinocyte migrationERK signaling |
| spellingShingle | Jinu Lee Seon Min Oh Hyung Won Ryu Jeong-Hwa Baek <i>Daphne kiusiana</i> Crude Extract and Its Fraction Enhance Keratinocyte Migration via the ERK/MMP9 Pathway Current Issues in Molecular Biology <i>Daphne kiusiana</i> phytomedicine wound healing keratinocyte migration ERK signaling |
| title | <i>Daphne kiusiana</i> Crude Extract and Its Fraction Enhance Keratinocyte Migration via the ERK/MMP9 Pathway |
| title_full | <i>Daphne kiusiana</i> Crude Extract and Its Fraction Enhance Keratinocyte Migration via the ERK/MMP9 Pathway |
| title_fullStr | <i>Daphne kiusiana</i> Crude Extract and Its Fraction Enhance Keratinocyte Migration via the ERK/MMP9 Pathway |
| title_full_unstemmed | <i>Daphne kiusiana</i> Crude Extract and Its Fraction Enhance Keratinocyte Migration via the ERK/MMP9 Pathway |
| title_short | <i>Daphne kiusiana</i> Crude Extract and Its Fraction Enhance Keratinocyte Migration via the ERK/MMP9 Pathway |
| title_sort | i daphne kiusiana i crude extract and its fraction enhance keratinocyte migration via the erk mmp9 pathway |
| topic | <i>Daphne kiusiana</i> phytomedicine wound healing keratinocyte migration ERK signaling |
| url | https://www.mdpi.com/1467-3045/47/5/300 |
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