CRISPR-Cas9 system in autosomal dominant polycystic kidney disease: a comprehensive review

Genetic kidney diseases are caused by mutations in specific genes that significantly affect kidney development and function. Although the underlying pathogenic genes of many kidney diseases have been identified, an understanding of their mechanisms and effective treatments remains limited. Gene edit...

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Main Authors: Seungyeon Kang, Se Jin Park, Min Ho Lee, Andreas Kronbichler, Jae Il Shin
Format: Article
Language:English
Published: Korean Society of Pediatric Nephrology 2025-02-01
Series:Childhood Kidney Diseases
Subjects:
Online Access:http://chikd.org/upload/ckd-25-008.pdf
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author Seungyeon Kang
Se Jin Park
Min Ho Lee
Andreas Kronbichler
Jae Il Shin
author_facet Seungyeon Kang
Se Jin Park
Min Ho Lee
Andreas Kronbichler
Jae Il Shin
author_sort Seungyeon Kang
collection DOAJ
description Genetic kidney diseases are caused by mutations in specific genes that significantly affect kidney development and function. Although the underlying pathogenic genes of many kidney diseases have been identified, an understanding of their mechanisms and effective treatments remains limited. Gene editing, particularly using clustered regularly interspaced short palindromic repeats (CRISPR), has recently become a promising approach for studying genetic diseases and the CRISPR/CRISPR-associated protein 9 (CRISPR-Cas9) method has become a prominent research method. It has been shown that CRISPR-Cas9 can be targeted to knock out specific genomic sites, which enables researchers to correct gene mutations, prevent inheritance, and better understand the function of genes and the effectiveness of drugs. However, the application of CRISPR-Cas9 technology in the development of therapeutic agents against genetic kidney disease has been overlooked compared with other genetic diseases. In this paper, we provide an overview of the current research advancements in genetic kidney diseases using CRISPR technology, as well as the diverse preclinical research methods implemented, with particular emphasis on autosomal dominant polycystic kidney disease.
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publishDate 2025-02-01
publisher Korean Society of Pediatric Nephrology
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series Childhood Kidney Diseases
spelling doaj-art-039e732bd983463fb5f200f355d4a1da2025-08-20T03:14:47ZengKorean Society of Pediatric NephrologyChildhood Kidney Diseases2384-02422384-02502025-02-0129141110.3339/ckd.25.008824CRISPR-Cas9 system in autosomal dominant polycystic kidney disease: a comprehensive reviewSeungyeon Kang0Se Jin Park1Min Ho Lee2Andreas Kronbichler3Jae Il Shin4 Department of Pharmacology and Toxicology, Molecular Genetics and Microbiology, University of Toronto, Toronto, ON, Canada Department of Pediatrics, Changwon Hanmaeum Hospital, Hanyang University College of Medicine, Changwon, Republic of Korea Department of Orthopedic Surgery, Yonsei University Health System, Seoul, Republic of Korea Department of Internal Medicine IV, Nephrology and Hypertension, Medical University of Innsbruck, Innsbruck, Austria Department of Pediatrics, Yonsei University College of Medicine, Seoul, Republic of KoreaGenetic kidney diseases are caused by mutations in specific genes that significantly affect kidney development and function. Although the underlying pathogenic genes of many kidney diseases have been identified, an understanding of their mechanisms and effective treatments remains limited. Gene editing, particularly using clustered regularly interspaced short palindromic repeats (CRISPR), has recently become a promising approach for studying genetic diseases and the CRISPR/CRISPR-associated protein 9 (CRISPR-Cas9) method has become a prominent research method. It has been shown that CRISPR-Cas9 can be targeted to knock out specific genomic sites, which enables researchers to correct gene mutations, prevent inheritance, and better understand the function of genes and the effectiveness of drugs. However, the application of CRISPR-Cas9 technology in the development of therapeutic agents against genetic kidney disease has been overlooked compared with other genetic diseases. In this paper, we provide an overview of the current research advancements in genetic kidney diseases using CRISPR technology, as well as the diverse preclinical research methods implemented, with particular emphasis on autosomal dominant polycystic kidney disease.http://chikd.org/upload/ckd-25-008.pdfautosomal dominant polycystic kidney diseaseclustered regularly interspaced short palindromic repeatscrispr-associated protein 9gene editingkidney
spellingShingle Seungyeon Kang
Se Jin Park
Min Ho Lee
Andreas Kronbichler
Jae Il Shin
CRISPR-Cas9 system in autosomal dominant polycystic kidney disease: a comprehensive review
Childhood Kidney Diseases
autosomal dominant polycystic kidney disease
clustered regularly interspaced short palindromic repeats
crispr-associated protein 9
gene editing
kidney
title CRISPR-Cas9 system in autosomal dominant polycystic kidney disease: a comprehensive review
title_full CRISPR-Cas9 system in autosomal dominant polycystic kidney disease: a comprehensive review
title_fullStr CRISPR-Cas9 system in autosomal dominant polycystic kidney disease: a comprehensive review
title_full_unstemmed CRISPR-Cas9 system in autosomal dominant polycystic kidney disease: a comprehensive review
title_short CRISPR-Cas9 system in autosomal dominant polycystic kidney disease: a comprehensive review
title_sort crispr cas9 system in autosomal dominant polycystic kidney disease a comprehensive review
topic autosomal dominant polycystic kidney disease
clustered regularly interspaced short palindromic repeats
crispr-associated protein 9
gene editing
kidney
url http://chikd.org/upload/ckd-25-008.pdf
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AT minholee crisprcas9systeminautosomaldominantpolycystickidneydiseaseacomprehensivereview
AT andreaskronbichler crisprcas9systeminautosomaldominantpolycystickidneydiseaseacomprehensivereview
AT jaeilshin crisprcas9systeminautosomaldominantpolycystickidneydiseaseacomprehensivereview