Antibiotic sensitivity analysis of clinical coagulase-negative staphylococci
Background. The increasing role of coagulase-negative staphylococci in the occurrence of staphylococcal infections leads to the need for close attention to them. Special control is required over the sensitivity of bacteria to antibiotics and the spread of methicillin resistance, as a sign of multipl...
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Scientific Сentre for Family Health and Human Reproduction Problems
2022-07-01
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| Series: | Acta Biomedica Scientifica |
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| Online Access: | https://www.actabiomedica.ru/jour/article/view/3553 |
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| author | L. I. Kononova L. M. Lemkina V. P. Korobov |
| author_facet | L. I. Kononova L. M. Lemkina V. P. Korobov |
| author_sort | L. I. Kononova |
| collection | DOAJ |
| description | Background. The increasing role of coagulase-negative staphylococci in the occurrence of staphylococcal infections leads to the need for close attention to them. Special control is required over the sensitivity of bacteria to antibiotics and the spread of methicillin resistance, as a sign of multiple resistance to antibacterial drugs. It is also important to identify the virulence factors of coagulase-negative staphylococci, which determine their behavior in the environment.The aim. To evaluate the sensitivity of strains of coagulase-negative staphylococci to clinically significant antibiotics daptomycin, vancomycin, linezolid and oxacillin and lantibiotic warnerin.Methods. Determination of the minimal inhibitory concentrations of antibacterial compounds for clinical coagulase-negative staphylococci by standard methods of serial dilutions and disc diffusion. Identification of the phenomenon of decreased susceptibility of bacteria to vancomycin by population analysis and concentration gradient. Lipid analysis by thin layer chromatography. Results. High antibacterial activity of vancomycin, daptomycin and linezolid against clinical strains of coagulase-negative staphylococci was shown. The upper limit of the minimum inhibitory concentrations of vancomycin within the sensitive phenotype and the expansion of the ranges of the minimum inhibitory concentrations of daptomycin and warnerin towards an increase in oxacillin-resistant isolates were revealed. The heterogeneous nature of sensitivity to vancomycin of the cultures of the studied strains and the possibility of their rapid enrichment with subpopulations with reduced sensitivity to this antibiotic have been established. The selection of resistance of coagulase-negative staphylococci to vancomycin was accompanied by an increase in the synthesis of lysylphosphatidylglycerol and a decrease in their sensitivity to cationic peptide compounds.Conclusion. The revealed prevalence of the methicillin-resistant phenotype of clinical strains of coagulase-negative staphylococci, along with the presence in the lipid spectrum of the universal factor of resistance to cationic antibacterial compounds, lysylphosphatidylglycerol, entails the need for new methodological solutions for diagnosing infections caused by coagulase-negative staphylococci. |
| format | Article |
| id | doaj-art-038f034231434ff8b18d5df383e2e5df |
| institution | Kabale University |
| issn | 2541-9420 2587-9596 |
| language | Russian |
| publishDate | 2022-07-01 |
| publisher | Scientific Сentre for Family Health and Human Reproduction Problems |
| record_format | Article |
| series | Acta Biomedica Scientifica |
| spelling | doaj-art-038f034231434ff8b18d5df383e2e5df2025-08-20T03:56:54ZrusScientific Сentre for Family Health and Human Reproduction ProblemsActa Biomedica Scientifica2541-94202587-95962022-07-0173758910.29413/ABS.2022-7.3.92345Antibiotic sensitivity analysis of clinical coagulase-negative staphylococciL. I. Kononova0L. M. Lemkina1V. P. Korobov2Ural Branch Russian Academy of Sciences Ural Branch Russian Academy of Sciences Perm National Research Polytechnic University; Ural Branch Russian Academy of Sciences Background. The increasing role of coagulase-negative staphylococci in the occurrence of staphylococcal infections leads to the need for close attention to them. Special control is required over the sensitivity of bacteria to antibiotics and the spread of methicillin resistance, as a sign of multiple resistance to antibacterial drugs. It is also important to identify the virulence factors of coagulase-negative staphylococci, which determine their behavior in the environment.The aim. To evaluate the sensitivity of strains of coagulase-negative staphylococci to clinically significant antibiotics daptomycin, vancomycin, linezolid and oxacillin and lantibiotic warnerin.Methods. Determination of the minimal inhibitory concentrations of antibacterial compounds for clinical coagulase-negative staphylococci by standard methods of serial dilutions and disc diffusion. Identification of the phenomenon of decreased susceptibility of bacteria to vancomycin by population analysis and concentration gradient. Lipid analysis by thin layer chromatography. Results. High antibacterial activity of vancomycin, daptomycin and linezolid against clinical strains of coagulase-negative staphylococci was shown. The upper limit of the minimum inhibitory concentrations of vancomycin within the sensitive phenotype and the expansion of the ranges of the minimum inhibitory concentrations of daptomycin and warnerin towards an increase in oxacillin-resistant isolates were revealed. The heterogeneous nature of sensitivity to vancomycin of the cultures of the studied strains and the possibility of their rapid enrichment with subpopulations with reduced sensitivity to this antibiotic have been established. The selection of resistance of coagulase-negative staphylococci to vancomycin was accompanied by an increase in the synthesis of lysylphosphatidylglycerol and a decrease in their sensitivity to cationic peptide compounds.Conclusion. The revealed prevalence of the methicillin-resistant phenotype of clinical strains of coagulase-negative staphylococci, along with the presence in the lipid spectrum of the universal factor of resistance to cationic antibacterial compounds, lysylphosphatidylglycerol, entails the need for new methodological solutions for diagnosing infections caused by coagulase-negative staphylococci.https://www.actabiomedica.ru/jour/article/view/3553heteroresistancevancomycinwarnerindaptomycinlantibioticslysylphosphatidylglycerollinezolidoxacillinstaphylococcihominin |
| spellingShingle | L. I. Kononova L. M. Lemkina V. P. Korobov Antibiotic sensitivity analysis of clinical coagulase-negative staphylococci Acta Biomedica Scientifica heteroresistance vancomycin warnerin daptomycin lantibiotics lysylphosphatidylglycerol linezolid oxacillin staphylococci hominin |
| title | Antibiotic sensitivity analysis of clinical coagulase-negative staphylococci |
| title_full | Antibiotic sensitivity analysis of clinical coagulase-negative staphylococci |
| title_fullStr | Antibiotic sensitivity analysis of clinical coagulase-negative staphylococci |
| title_full_unstemmed | Antibiotic sensitivity analysis of clinical coagulase-negative staphylococci |
| title_short | Antibiotic sensitivity analysis of clinical coagulase-negative staphylococci |
| title_sort | antibiotic sensitivity analysis of clinical coagulase negative staphylococci |
| topic | heteroresistance vancomycin warnerin daptomycin lantibiotics lysylphosphatidylglycerol linezolid oxacillin staphylococci hominin |
| url | https://www.actabiomedica.ru/jour/article/view/3553 |
| work_keys_str_mv | AT likononova antibioticsensitivityanalysisofclinicalcoagulasenegativestaphylococci AT lmlemkina antibioticsensitivityanalysisofclinicalcoagulasenegativestaphylococci AT vpkorobov antibioticsensitivityanalysisofclinicalcoagulasenegativestaphylococci |