Field Deployment of a Mobile Biosafety Laboratory Reveals the Co-Circulation of Dengue Viruses Serotype 1 and Serotype 2 in Louga City, Senegal, 2017
Dengue virus (DENV) is the most prevalent arboviral threat worldwide. This virus belonging to genus Flavivirus, Flaviviridae family, is responsible for a wide spectrum of clinical manifestations, ranging from asymptomatic or mild febrile illness (dengue fever) to life-threatening infections (severe...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Journal of Tropical Medicine |
| Online Access: | http://dx.doi.org/10.1155/2021/8817987 |
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| author | Idrissa Dieng Maryam Diarra Moussa Moïse Diagne Martin Faye Marie Henriette Dior Ndione Yamar Ba Mamadou Diop El Hadji Ndiaye Paolo Marinho de Andrade Zanotto Boly Diop Mamadou Ndiaye Abdoulaye Bousso Ndongo Dia Mawlouth Diallo Aliou Barry Gamou Fall Cheikh Loucoubar Amadou Alpha Sall Ousmane Faye Oumar Faye |
| author_facet | Idrissa Dieng Maryam Diarra Moussa Moïse Diagne Martin Faye Marie Henriette Dior Ndione Yamar Ba Mamadou Diop El Hadji Ndiaye Paolo Marinho de Andrade Zanotto Boly Diop Mamadou Ndiaye Abdoulaye Bousso Ndongo Dia Mawlouth Diallo Aliou Barry Gamou Fall Cheikh Loucoubar Amadou Alpha Sall Ousmane Faye Oumar Faye |
| author_sort | Idrissa Dieng |
| collection | DOAJ |
| description | Dengue virus (DENV) is the most prevalent arboviral threat worldwide. This virus belonging to genus Flavivirus, Flaviviridae family, is responsible for a wide spectrum of clinical manifestations, ranging from asymptomatic or mild febrile illness (dengue fever) to life-threatening infections (severe dengue). Many sporadic cases and outbreaks have occurred in Senegal since 1970. Nevertheless, this article describes a field investigation of suspected dengue cases, between 05 September 2017 and 17 December 2017 made possible by the deployment of a Mobile Biosafety Laboratory (MBS-Lab). Overall, 960 human sera were collected and tested in the field for the presence of viral RNA by real-time RT-PCR. Serotyping, sequencing of complete E gene, and phylogenetic analysis were also performed. Out of 960 suspected cases, 131 were confirmed dengue cases. The majority of confirmed cases were from Louga community. Serotyping revealed two serotypes, Dengue 1 (100/104; 96, 15%) and Dengue 2 (04/104; 3, 84%). Phylogenetic analysis of the sequences obtained indicated that the Dengue 1 strain was closely related to strains isolated, respectively, in Singapore (Asia) in 2013 (KX380803.1) outbreak and it cocirculated with a Dengue 2 strain closely related to strains from a Burkina Faso dengue outbreak in 2016 (KY62776.1). Our results showed the co-circulation of two dengue virus serotypes during a single outbreak in a short time period. This co-circulation highlighted the need to improve surveillance in order to prevent future potential severe dengue cases through antibody-dependent enhancement (ADE). Interestingly, it also proved the reliability and usefulness of the MBS-Lab for expedient outbreak response at the point of need, which allows early cases management. |
| format | Article |
| id | doaj-art-038cbe84d11e4ba5b095a5b5c63f9df7 |
| institution | Kabale University |
| issn | 1687-9686 1687-9694 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Tropical Medicine |
| spelling | doaj-art-038cbe84d11e4ba5b095a5b5c63f9df72025-02-03T05:49:15ZengWileyJournal of Tropical Medicine1687-96861687-96942021-01-01202110.1155/2021/88179878817987Field Deployment of a Mobile Biosafety Laboratory Reveals the Co-Circulation of Dengue Viruses Serotype 1 and Serotype 2 in Louga City, Senegal, 2017Idrissa Dieng0Maryam Diarra1Moussa Moïse Diagne2Martin Faye3Marie Henriette Dior Ndione4Yamar Ba5Mamadou Diop6El Hadji Ndiaye7Paolo Marinho de Andrade Zanotto8Boly Diop9Mamadou Ndiaye10Abdoulaye Bousso11Ndongo Dia12Mawlouth Diallo13Aliou Barry14Gamou Fall15Cheikh Loucoubar16Amadou Alpha Sall17Ousmane Faye18Oumar Faye19Arboviruses and Haemorrhagic Fever Viruses Unit, Virology Department, Institut Pasteur de Dakar, Dakar 220, SenegalEpidemiology, Clinical Research and Data Science Unit, Institut Pasteur de Dakar, Dakar 220, SenegalArboviruses and Haemorrhagic Fever Viruses Unit, Virology Department, Institut Pasteur de Dakar, Dakar 220, SenegalArboviruses and Haemorrhagic Fever Viruses Unit, Virology Department, Institut Pasteur de Dakar, Dakar 220, SenegalArboviruses and Haemorrhagic Fever Viruses Unit, Virology Department, Institut Pasteur de Dakar, Dakar 220, SenegalMedical Entomology Unit, Institut Pasteur de Dakar, Dakar, 36 Avenue Pasteur, SenegalEpidemiology, Clinical Research and Data Science Unit, Institut Pasteur de Dakar, Dakar 220, SenegalMedical Entomology Unit, Institut Pasteur de Dakar, Dakar, 36 Avenue Pasteur, SenegalLaboratory of Molecular Evolution and Bioinformatics, Department of Microbiology, Biomedical Sciences Institute, University of SP, SP, BrazilPrevention Department, Ministry of Health, Dakar, SenegalPrevention Department, Ministry of Health, Dakar, SenegalPrevention Department, Ministry of Health, Dakar, SenegalArboviruses and Haemorrhagic Fever Viruses Unit, Virology Department, Institut Pasteur de Dakar, Dakar 220, SenegalMedical Entomology Unit, Institut Pasteur de Dakar, Dakar, 36 Avenue Pasteur, SenegalLaboratory of Molecular Evolution and Bioinformatics, Department of Microbiology, Biomedical Sciences Institute, University of SP, SP, BrazilArboviruses and Haemorrhagic Fever Viruses Unit, Virology Department, Institut Pasteur de Dakar, Dakar 220, SenegalEpidemiology, Clinical Research and Data Science Unit, Institut Pasteur de Dakar, Dakar 220, SenegalArboviruses and Haemorrhagic Fever Viruses Unit, Virology Department, Institut Pasteur de Dakar, Dakar 220, SenegalArboviruses and Haemorrhagic Fever Viruses Unit, Virology Department, Institut Pasteur de Dakar, Dakar 220, SenegalArboviruses and Haemorrhagic Fever Viruses Unit, Virology Department, Institut Pasteur de Dakar, Dakar 220, SenegalDengue virus (DENV) is the most prevalent arboviral threat worldwide. This virus belonging to genus Flavivirus, Flaviviridae family, is responsible for a wide spectrum of clinical manifestations, ranging from asymptomatic or mild febrile illness (dengue fever) to life-threatening infections (severe dengue). Many sporadic cases and outbreaks have occurred in Senegal since 1970. Nevertheless, this article describes a field investigation of suspected dengue cases, between 05 September 2017 and 17 December 2017 made possible by the deployment of a Mobile Biosafety Laboratory (MBS-Lab). Overall, 960 human sera were collected and tested in the field for the presence of viral RNA by real-time RT-PCR. Serotyping, sequencing of complete E gene, and phylogenetic analysis were also performed. Out of 960 suspected cases, 131 were confirmed dengue cases. The majority of confirmed cases were from Louga community. Serotyping revealed two serotypes, Dengue 1 (100/104; 96, 15%) and Dengue 2 (04/104; 3, 84%). Phylogenetic analysis of the sequences obtained indicated that the Dengue 1 strain was closely related to strains isolated, respectively, in Singapore (Asia) in 2013 (KX380803.1) outbreak and it cocirculated with a Dengue 2 strain closely related to strains from a Burkina Faso dengue outbreak in 2016 (KY62776.1). Our results showed the co-circulation of two dengue virus serotypes during a single outbreak in a short time period. This co-circulation highlighted the need to improve surveillance in order to prevent future potential severe dengue cases through antibody-dependent enhancement (ADE). Interestingly, it also proved the reliability and usefulness of the MBS-Lab for expedient outbreak response at the point of need, which allows early cases management.http://dx.doi.org/10.1155/2021/8817987 |
| spellingShingle | Idrissa Dieng Maryam Diarra Moussa Moïse Diagne Martin Faye Marie Henriette Dior Ndione Yamar Ba Mamadou Diop El Hadji Ndiaye Paolo Marinho de Andrade Zanotto Boly Diop Mamadou Ndiaye Abdoulaye Bousso Ndongo Dia Mawlouth Diallo Aliou Barry Gamou Fall Cheikh Loucoubar Amadou Alpha Sall Ousmane Faye Oumar Faye Field Deployment of a Mobile Biosafety Laboratory Reveals the Co-Circulation of Dengue Viruses Serotype 1 and Serotype 2 in Louga City, Senegal, 2017 Journal of Tropical Medicine |
| title | Field Deployment of a Mobile Biosafety Laboratory Reveals the Co-Circulation of Dengue Viruses Serotype 1 and Serotype 2 in Louga City, Senegal, 2017 |
| title_full | Field Deployment of a Mobile Biosafety Laboratory Reveals the Co-Circulation of Dengue Viruses Serotype 1 and Serotype 2 in Louga City, Senegal, 2017 |
| title_fullStr | Field Deployment of a Mobile Biosafety Laboratory Reveals the Co-Circulation of Dengue Viruses Serotype 1 and Serotype 2 in Louga City, Senegal, 2017 |
| title_full_unstemmed | Field Deployment of a Mobile Biosafety Laboratory Reveals the Co-Circulation of Dengue Viruses Serotype 1 and Serotype 2 in Louga City, Senegal, 2017 |
| title_short | Field Deployment of a Mobile Biosafety Laboratory Reveals the Co-Circulation of Dengue Viruses Serotype 1 and Serotype 2 in Louga City, Senegal, 2017 |
| title_sort | field deployment of a mobile biosafety laboratory reveals the co circulation of dengue viruses serotype 1 and serotype 2 in louga city senegal 2017 |
| url | http://dx.doi.org/10.1155/2021/8817987 |
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