Differential expression and molecular genetic characteristics of CD36 antigen in platelet donor database in Shaanxi province, China
[Objective] To screen the frequency of CD36 antigen expression in platelet donor database in Shaanxi province and analyze the molecular genetic characteristics of samples with CD36 antigen deficiency and low expression. [Methods] A total of 525 platelet donors samples were randomly collected during...
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| Format: | Article |
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Institute of Blood Transfusion of Chinese Academy of Medical Sciences
2025-05-01
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| Series: | Zhongguo shuxue zazhi |
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| Online Access: | https://www.cjbt.cn/thesisDetails#10.13303/j.cjbt.issn.1004-549x.2025.05.005&lang=en |
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| _version_ | 1850141769571762176 |
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| author | WANG Tianju QI Jun WANG Manni LI Yuhui SHANG Lixia CHEN Le WANG Xiaofang |
| author_facet | WANG Tianju QI Jun WANG Manni LI Yuhui SHANG Lixia CHEN Le WANG Xiaofang |
| author_sort | WANG Tianju |
| collection | DOAJ |
| description | [Objective] To screen the frequency of CD36 antigen expression in platelet donor database in Shaanxi province and analyze the molecular genetic characteristics of samples with CD36 antigen deficiency and low expression. [Methods] A total of 525 platelet donors samples were randomly collected during May 2023. CD36-FITC monoclonal antibody was used for immunofluorescence labeling, and flow cytometry was applied to detect the expression of CD36 antigen on platelets. For samples with CD36 antigen deficiency on platelets, the expression of CD36 on monocytes was further detected. Samples with CD36 antigen deficiency and low expression were sequenced and analyzed. [Results] Among the 525 blood samples, 99.24% (521/525) showed positive expression of CD36 antigen. There were differences in the expression intensity of CD36 antigen, with low expression accounting for 3.43% (18/525) and CD36 antigen deficiency accounting for 0.76% (4/525), all of which were type Ⅱ deficiency. The exon mutation frequency of CD36 type Ⅱ deficiency and low expression samples was 31.82% (7/22), and the exon mutation types were 121-1_126delGCAAGTT, 329-330delAC, 1142T>G, 1204-1246dupl 43bp, 1221G>A, and 1228-1239delATTGTGCCTATT. All four cases of CD36 type Ⅱ deficiency had a 121-6 T>C mutation in intron 3. All CD36 low expression samples had a mutation of 282-10A>G, and 121-6T>C mutation rate was 61.1%(11/18). [Conclusion] There were differences in the expression of CD36 antigen in the platelet donor database in Shaanxi province, which may be caused by multiple molecular genetic variations. The frequency of CD36 antigen deficiency in Shaanxi was lower than that of Han, Zhuang and Yao populations in southern China. This study provides references for solving the CD36 antibody mediated transfusion reaction and auxiliary treatment of diseases caused by CD36 antigen deficiency in the future. It also provides a basis for investigating the molecular mechanisms of CD36 deficiency and low expression. |
| format | Article |
| id | doaj-art-0380e178b27c4be8bd689c1edf670f68 |
| institution | OA Journals |
| issn | 1004-549X |
| language | zho |
| publishDate | 2025-05-01 |
| publisher | Institute of Blood Transfusion of Chinese Academy of Medical Sciences |
| record_format | Article |
| series | Zhongguo shuxue zazhi |
| spelling | doaj-art-0380e178b27c4be8bd689c1edf670f682025-08-20T02:29:19ZzhoInstitute of Blood Transfusion of Chinese Academy of Medical SciencesZhongguo shuxue zazhi1004-549X2025-05-0138562162810.13303/j.cjbt.issn.1004-549x.2025.05.0051004-549X(2025)5-0621-08Differential expression and molecular genetic characteristics of CD36 antigen in platelet donor database in Shaanxi province, ChinaWANG Tianju0QI Jun1WANG Manni2LI Yuhui3SHANG Lixia4CHEN Le5WANG Xiaofang6Shaanxi Blood Center, Xi'an Blood Center, Xi'an 710061, ChinaShaanxi Blood Center, Xi'an Blood Center, Xi'an 710061, ChinaShaanxi Blood Center, Xi'an Blood Center, Xi'an 710061, ChinaShaanxi Blood Center, Xi'an Blood Center, Xi'an 710061, ChinaShaanxi Blood Center, Xi'an Blood Center, Xi'an 710061, ChinaShaanxi Blood Center, Xi'an Blood Center, Xi'an 710061, ChinaShaanxi Blood Center, Xi'an Blood Center, Xi'an 710061, China[Objective] To screen the frequency of CD36 antigen expression in platelet donor database in Shaanxi province and analyze the molecular genetic characteristics of samples with CD36 antigen deficiency and low expression. [Methods] A total of 525 platelet donors samples were randomly collected during May 2023. CD36-FITC monoclonal antibody was used for immunofluorescence labeling, and flow cytometry was applied to detect the expression of CD36 antigen on platelets. For samples with CD36 antigen deficiency on platelets, the expression of CD36 on monocytes was further detected. Samples with CD36 antigen deficiency and low expression were sequenced and analyzed. [Results] Among the 525 blood samples, 99.24% (521/525) showed positive expression of CD36 antigen. There were differences in the expression intensity of CD36 antigen, with low expression accounting for 3.43% (18/525) and CD36 antigen deficiency accounting for 0.76% (4/525), all of which were type Ⅱ deficiency. The exon mutation frequency of CD36 type Ⅱ deficiency and low expression samples was 31.82% (7/22), and the exon mutation types were 121-1_126delGCAAGTT, 329-330delAC, 1142T>G, 1204-1246dupl 43bp, 1221G>A, and 1228-1239delATTGTGCCTATT. All four cases of CD36 type Ⅱ deficiency had a 121-6 T>C mutation in intron 3. All CD36 low expression samples had a mutation of 282-10A>G, and 121-6T>C mutation rate was 61.1%(11/18). [Conclusion] There were differences in the expression of CD36 antigen in the platelet donor database in Shaanxi province, which may be caused by multiple molecular genetic variations. The frequency of CD36 antigen deficiency in Shaanxi was lower than that of Han, Zhuang and Yao populations in southern China. This study provides references for solving the CD36 antibody mediated transfusion reaction and auxiliary treatment of diseases caused by CD36 antigen deficiency in the future. It also provides a basis for investigating the molecular mechanisms of CD36 deficiency and low expression.https://www.cjbt.cn/thesisDetails#10.13303/j.cjbt.issn.1004-549x.2025.05.005&lang=encd36 antigen type ⅱ deficiencycd36 antigen low expressiongene mutationexonintronsplice site region |
| spellingShingle | WANG Tianju QI Jun WANG Manni LI Yuhui SHANG Lixia CHEN Le WANG Xiaofang Differential expression and molecular genetic characteristics of CD36 antigen in platelet donor database in Shaanxi province, China Zhongguo shuxue zazhi cd36 antigen type ⅱ deficiency cd36 antigen low expression gene mutation exon intron splice site region |
| title | Differential expression and molecular genetic characteristics of CD36 antigen in platelet donor database in Shaanxi province, China |
| title_full | Differential expression and molecular genetic characteristics of CD36 antigen in platelet donor database in Shaanxi province, China |
| title_fullStr | Differential expression and molecular genetic characteristics of CD36 antigen in platelet donor database in Shaanxi province, China |
| title_full_unstemmed | Differential expression and molecular genetic characteristics of CD36 antigen in platelet donor database in Shaanxi province, China |
| title_short | Differential expression and molecular genetic characteristics of CD36 antigen in platelet donor database in Shaanxi province, China |
| title_sort | differential expression and molecular genetic characteristics of cd36 antigen in platelet donor database in shaanxi province china |
| topic | cd36 antigen type ⅱ deficiency cd36 antigen low expression gene mutation exon intron splice site region |
| url | https://www.cjbt.cn/thesisDetails#10.13303/j.cjbt.issn.1004-549x.2025.05.005&lang=en |
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