Epidemiology and Molecular Identification of Dermatophytes: Focus on the Detection of the Emerging Species <i>Trichophyton indotineae</i> in Northern Italy

Epidemiology and Molecular Identification of Dermatophytes: Focus on the Detection of the Emerging Species <i>Trichophyton indotineae</i> in Northern Italy

<b>Background:</b> <i>Trichophyton indotineae</i>, a new emerging pathogen according to the WHO, is known to cause severe forms of tinea. Given that traditional identification methods rely on morphological characteristics, and the morphological distinctions among <i>T....

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Main Authors: Valentina Lepera, Gabriella Tocci, Giorgia Palladini, Marco Enrico Giovanni Arosio, Claudio Farina, Giuliana Lo Cascio, on behalf of the Medical Mycology Committee (CoSM)—Italian Association of Clinical Microbiology (AMCLI)
Format: Article
Language:English
Published: MDPI AG 2025-07-01
Series:Microbiology Research
Subjects:
<i>Trichophyton indotineae</i>
dermatophytes
medical mycology
terbinafine resistance
Italy
Online Access:https://www.mdpi.com/2036-7481/16/7/148
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Summary:<b>Background:</b> <i>Trichophyton indotineae</i>, a new emerging pathogen according to the WHO, is known to cause severe forms of tinea. Given that traditional identification methods rely on morphological characteristics, and the morphological distinctions among <i>T. indotineae</i>, <i>T. mentagrophytes</i>, and <i>T. interdigitale</i> are minimal, the adoption of alternative diagnostic techniques, such as RT-PCR or gene sequencing, has become critically important to prevent misidentification. The purpose of this study was firstly to analyze the local epidemiology of dermatophytes isolated and secondly to investigate the presence of <i>T. indotineae</i> among the isolated strains. <b>Methods</b>: Between January 2021 and June 2024, 1096 samples of skin adnexa were analysed. The isolated strains belonging to the genus <i>Trichophyton</i> were submitted to molecular identification by ITS sequencing, and <i>T. indotineae</i> strains were subjected to squalene epoxidase (SQLE) sequencing analysis. <b>Results</b>: <i>Trichophyton rubrum</i> and <i>Trichophyton interdigitale</i> appear to be the most prevalent pathogenic species. Molecular identification reveals four <i>T. indotineae</i> strains (4/87; 4.61%) from Asian patients, which were also characterized by gene mutations associated with terbinafine resistance. <b>Conclusions</b>: This study has made it clear that there is a need to implement basic mycological diagnostics with molecular methods to avoid misidentifications, ensure the correct identification, and evaluate the presence of mutations associated with antifungal drug resistance.
ISSN:2036-7481

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