Occurrence of Clostridium difficile infections due to PCR ribotype 027 in Bucharest, Romania
Introduction: Little is known about prevailing ribotypes of Clostridium difficile infection in Romania where CDI is not a mandatory notifiable disease. Methodology: We studied 64 non-duplicate C. difficile isolates from patients hospitalised at the National Institute of Infectious Diseases, Buchare...
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The Journal of Infection in Developing Countries
2014-06-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/4435 |
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| author | Alexandru Rafila Alexander Indra Gabriel Adrian Popescu Günther Wewalka Franz Allerberger Serban Benea Ioana Badicut Richard Aschbacher Steliana Huhulescu |
| author_facet | Alexandru Rafila Alexander Indra Gabriel Adrian Popescu Günther Wewalka Franz Allerberger Serban Benea Ioana Badicut Richard Aschbacher Steliana Huhulescu |
| author_sort | Alexandru Rafila |
| collection | DOAJ |
| description | Introduction: Little is known about prevailing ribotypes of Clostridium difficile infection in Romania where CDI is not a mandatory notifiable disease.
Methodology: We studied 64 non-duplicate C. difficile isolates from patients hospitalised at the National Institute of Infectious Diseases, Bucharest, Romania between March 2011 and March 2012.
Results: Sixty-three of the 64 C. difficile isolates produced toxins A and B whereas 44 (69%) isolates produced a binary toxin. Ribotype 027 accounted for 43 (68%) of the 63 toxigenic strains. The remaining 20 isolates belonged to ribotypes 018 (n = 9), 012 (n = 3), and, with one isolate each, 014, 031, 081, 416, 433, 500, 507 and PR03035 (new ribotype). Information on hospital mortality was available for 62 of the 64 patients; among these 62 cases, 4 (6.4%) ended fatal. Recurrence was documented for 11 (18.3%) of the 60 patients for whom this information was available. Multilocus variable-number tandem repeat analysis of the 43 isolates of ribotype 027 yielded a unique cluster for the Romanian isolates when compared to Austrian or Italian isolates.
Conclusion: Our findings sustain the hypothesis of a recent emerged outbreak of C. difficile PCR ribotype 027 infections in the area of Bucharest.
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| format | Article |
| id | doaj-art-03597364b58f4bf2ac84c30e840d00c8 |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2014-06-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-03597364b58f4bf2ac84c30e840d00c82025-08-20T02:27:15ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802014-06-0180610.3855/jidc.4435Occurrence of Clostridium difficile infections due to PCR ribotype 027 in Bucharest, RomaniaAlexandru Rafila0Alexander Indra1Gabriel Adrian Popescu2Günther Wewalka3Franz Allerberger4Serban Benea5Ioana Badicut6Richard Aschbacher7Steliana Huhulescu8National Institute for Infectious Diseases Matei Bals, Bucharest, RomaniaInstitute for Medical Microbiologyand Hygiene,Vienna, AustriaNational Institute for Infectious Diseases Matei Bals, Bucharest, RomaniaInstitute for Medical Microbiologyand Hygiene,Vienna, AustriaInstitute for Medical Microbiologyand Hygiene,Vienna, AustriaNational Institute for Infectious Diseases Matei Bals, Bucharest, RomaniaNational Institute for Infectious Diseases Matei Bals, Bucharest, RomaniaLaboratorio Aziendale di Microbiologia e Virologia, Comprensorio Sanitario di BolzanoLaboratorio Aziendale di Microbiologia e Virologia, Comprensorio Sanitario di Bolzano, ItalyIntroduction: Little is known about prevailing ribotypes of Clostridium difficile infection in Romania where CDI is not a mandatory notifiable disease. Methodology: We studied 64 non-duplicate C. difficile isolates from patients hospitalised at the National Institute of Infectious Diseases, Bucharest, Romania between March 2011 and March 2012. Results: Sixty-three of the 64 C. difficile isolates produced toxins A and B whereas 44 (69%) isolates produced a binary toxin. Ribotype 027 accounted for 43 (68%) of the 63 toxigenic strains. The remaining 20 isolates belonged to ribotypes 018 (n = 9), 012 (n = 3), and, with one isolate each, 014, 031, 081, 416, 433, 500, 507 and PR03035 (new ribotype). Information on hospital mortality was available for 62 of the 64 patients; among these 62 cases, 4 (6.4%) ended fatal. Recurrence was documented for 11 (18.3%) of the 60 patients for whom this information was available. Multilocus variable-number tandem repeat analysis of the 43 isolates of ribotype 027 yielded a unique cluster for the Romanian isolates when compared to Austrian or Italian isolates. Conclusion: Our findings sustain the hypothesis of a recent emerged outbreak of C. difficile PCR ribotype 027 infections in the area of Bucharest. https://jidc.org/index.php/journal/article/view/4435Clostridium difficileribotype 027Romania |
| spellingShingle | Alexandru Rafila Alexander Indra Gabriel Adrian Popescu Günther Wewalka Franz Allerberger Serban Benea Ioana Badicut Richard Aschbacher Steliana Huhulescu Occurrence of Clostridium difficile infections due to PCR ribotype 027 in Bucharest, Romania Journal of Infection in Developing Countries Clostridium difficile ribotype 027 Romania |
| title | Occurrence of Clostridium difficile infections due to PCR ribotype 027 in Bucharest, Romania |
| title_full | Occurrence of Clostridium difficile infections due to PCR ribotype 027 in Bucharest, Romania |
| title_fullStr | Occurrence of Clostridium difficile infections due to PCR ribotype 027 in Bucharest, Romania |
| title_full_unstemmed | Occurrence of Clostridium difficile infections due to PCR ribotype 027 in Bucharest, Romania |
| title_short | Occurrence of Clostridium difficile infections due to PCR ribotype 027 in Bucharest, Romania |
| title_sort | occurrence of clostridium difficile infections due to pcr ribotype 027 in bucharest romania |
| topic | Clostridium difficile ribotype 027 Romania |
| url | https://jidc.org/index.php/journal/article/view/4435 |
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