Detection and Phylogenetic Analysis of Alphacoronaviruses in Bat Populations of Rostov and Novosibirsk Regions of Russia, 2021–2023
Bats are natural reservoirs for many emerging viruses, including coronaviruses that were probably progenitors to human coronaviruses with epidemic and pandemic potential, which highlights the importance of screening studies of bat-derived viruses. This study investigates the prevalence and phylogene...
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2024-12-01
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| author | Olesia V. Ohlopkova Igor V. Popov Ilia V. Popov Kristina A. Stolbunova Marina A. Stepanyuk Alexey D. Moshkin Aleksei A. Maslov Ivan A. Sobolev Aleksey V. Malinovkin Elizaveta V. Tkacheva Daria A. Bogdanova Ekaterina A. Lukbanova Alexey M. Ermakov Alexander Y. Alekseev Svetoslav D. Todorov Alexander M. Shestopalov |
| author_facet | Olesia V. Ohlopkova Igor V. Popov Ilia V. Popov Kristina A. Stolbunova Marina A. Stepanyuk Alexey D. Moshkin Aleksei A. Maslov Ivan A. Sobolev Aleksey V. Malinovkin Elizaveta V. Tkacheva Daria A. Bogdanova Ekaterina A. Lukbanova Alexey M. Ermakov Alexander Y. Alekseev Svetoslav D. Todorov Alexander M. Shestopalov |
| author_sort | Olesia V. Ohlopkova |
| collection | DOAJ |
| description | Bats are natural reservoirs for many emerging viruses, including coronaviruses that were probably progenitors to human coronaviruses with epidemic and pandemic potential, which highlights the importance of screening studies of bat-derived viruses. This study investigates the prevalence and phylogenetic characteristics of coronaviruses in bat populations from the Rostov and Novosibirsk regions of Russia between 2021 and 2023. Utilizing PCR screening and sequencing, viruses belonging to the <i>Alphacoronavirus</i> genus were detected in several bat species, with prevalence rates ranging from 4.94% to 62.5%. Phylogenetic analysis of detected sequences revealed the presence of three subgenera: <i>Pedacovirus</i>, <i>Myotacovirus</i>, and <i>Nyctacovirus</i>. These sequences shared over 90% identity with alphacoronaviruses previously identified in bats across Northern Europe and Russia, underscoring the viruses’ wide geographic distribution and evolutionary connections. The results highlight the adaptability of alphacoronaviruses and the role of bat migratory behavior in their dispersal. The study underscores the importance of continuous monitoring and phylogenetic studies of bat-derived coronaviruses to better understand their ecological dynamics and potential zoonotic threats. |
| format | Article |
| id | doaj-art-034eecb38f9c491b8418f543e6e8b2bc |
| institution | Kabale University |
| issn | 2036-7481 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microbiology Research |
| spelling | doaj-art-034eecb38f9c491b8418f543e6e8b2bc2025-01-24T13:41:42ZengMDPI AGMicrobiology Research2036-74812024-12-01161310.3390/microbiolres16010003Detection and Phylogenetic Analysis of Alphacoronaviruses in Bat Populations of Rostov and Novosibirsk Regions of Russia, 2021–2023Olesia V. Ohlopkova0Igor V. Popov1Ilia V. Popov2Kristina A. Stolbunova3Marina A. Stepanyuk4Alexey D. Moshkin5Aleksei A. Maslov6Ivan A. Sobolev7Aleksey V. Malinovkin8Elizaveta V. Tkacheva9Daria A. Bogdanova10Ekaterina A. Lukbanova11Alexey M. Ermakov12Alexander Y. Alekseev13Svetoslav D. Todorov14Alexander M. Shestopalov15Research Institute of Virology, Federal State Budgetary Scientific Institution “Federal Research Center for Fundamental and Translational Medicine”, Novosibirsk 630117, RussiaFaculty “Bioengineering and Veterinary Medicine”, Don State Technical University, Rostov-on-Don 344000, RussiaFaculty “Bioengineering and Veterinary Medicine”, Don State Technical University, Rostov-on-Don 344000, RussiaResearch Institute of Virology, Federal State Budgetary Scientific Institution “Federal Research Center for Fundamental and Translational Medicine”, Novosibirsk 630117, RussiaResearch Institute of Virology, Federal State Budgetary Scientific Institution “Federal Research Center for Fundamental and Translational Medicine”, Novosibirsk 630117, RussiaResearch Institute of Virology, Federal State Budgetary Scientific Institution “Federal Research Center for Fundamental and Translational Medicine”, Novosibirsk 630117, RussiaResearch Institute of Virology, Federal State Budgetary Scientific Institution “Federal Research Center for Fundamental and Translational Medicine”, Novosibirsk 630117, RussiaResearch Institute of Virology, Federal State Budgetary Scientific Institution “Federal Research Center for Fundamental and Translational Medicine”, Novosibirsk 630117, RussiaFaculty “Bioengineering and Veterinary Medicine”, Don State Technical University, Rostov-on-Don 344000, RussiaResearch Institute of Virology, Federal State Budgetary Scientific Institution “Federal Research Center for Fundamental and Translational Medicine”, Novosibirsk 630117, RussiaDivision of Immunobiology and Biomedicine, Center of Genetics and Life Sciences, Sirius University of Science and Technology, Federal Territory Sirius 354340, RussiaFaculty “Bioengineering and Veterinary Medicine”, Don State Technical University, Rostov-on-Don 344000, RussiaFaculty “Bioengineering and Veterinary Medicine”, Don State Technical University, Rostov-on-Don 344000, RussiaResearch Institute of Virology, Federal State Budgetary Scientific Institution “Federal Research Center for Fundamental and Translational Medicine”, Novosibirsk 630117, RussiaProBacLab, Laboratório de Microbiologia de Alimentos, Departamento de Alimentos e Nutrição Experimental, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, São Paulo 05508-000, SP, BrazilResearch Institute of Virology, Federal State Budgetary Scientific Institution “Federal Research Center for Fundamental and Translational Medicine”, Novosibirsk 630117, RussiaBats are natural reservoirs for many emerging viruses, including coronaviruses that were probably progenitors to human coronaviruses with epidemic and pandemic potential, which highlights the importance of screening studies of bat-derived viruses. This study investigates the prevalence and phylogenetic characteristics of coronaviruses in bat populations from the Rostov and Novosibirsk regions of Russia between 2021 and 2023. Utilizing PCR screening and sequencing, viruses belonging to the <i>Alphacoronavirus</i> genus were detected in several bat species, with prevalence rates ranging from 4.94% to 62.5%. Phylogenetic analysis of detected sequences revealed the presence of three subgenera: <i>Pedacovirus</i>, <i>Myotacovirus</i>, and <i>Nyctacovirus</i>. These sequences shared over 90% identity with alphacoronaviruses previously identified in bats across Northern Europe and Russia, underscoring the viruses’ wide geographic distribution and evolutionary connections. The results highlight the adaptability of alphacoronaviruses and the role of bat migratory behavior in their dispersal. The study underscores the importance of continuous monitoring and phylogenetic studies of bat-derived coronaviruses to better understand their ecological dynamics and potential zoonotic threats.https://www.mdpi.com/2036-7481/16/1/3batscoronavirusesPCRsequencingphylogeneticsepizootology |
| spellingShingle | Olesia V. Ohlopkova Igor V. Popov Ilia V. Popov Kristina A. Stolbunova Marina A. Stepanyuk Alexey D. Moshkin Aleksei A. Maslov Ivan A. Sobolev Aleksey V. Malinovkin Elizaveta V. Tkacheva Daria A. Bogdanova Ekaterina A. Lukbanova Alexey M. Ermakov Alexander Y. Alekseev Svetoslav D. Todorov Alexander M. Shestopalov Detection and Phylogenetic Analysis of Alphacoronaviruses in Bat Populations of Rostov and Novosibirsk Regions of Russia, 2021–2023 Microbiology Research bats coronaviruses PCR sequencing phylogenetics epizootology |
| title | Detection and Phylogenetic Analysis of Alphacoronaviruses in Bat Populations of Rostov and Novosibirsk Regions of Russia, 2021–2023 |
| title_full | Detection and Phylogenetic Analysis of Alphacoronaviruses in Bat Populations of Rostov and Novosibirsk Regions of Russia, 2021–2023 |
| title_fullStr | Detection and Phylogenetic Analysis of Alphacoronaviruses in Bat Populations of Rostov and Novosibirsk Regions of Russia, 2021–2023 |
| title_full_unstemmed | Detection and Phylogenetic Analysis of Alphacoronaviruses in Bat Populations of Rostov and Novosibirsk Regions of Russia, 2021–2023 |
| title_short | Detection and Phylogenetic Analysis of Alphacoronaviruses in Bat Populations of Rostov and Novosibirsk Regions of Russia, 2021–2023 |
| title_sort | detection and phylogenetic analysis of alphacoronaviruses in bat populations of rostov and novosibirsk regions of russia 2021 2023 |
| topic | bats coronaviruses PCR sequencing phylogenetics epizootology |
| url | https://www.mdpi.com/2036-7481/16/1/3 |
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