Optimizing mycelial growth of Trametes versicolor HBB 7328 for enzyme production and green energy
Trametes versicolor, a common upper polyporus fungus, demonstrates mycelial growth that is influenced by temperature, pH, and media composition. This study aimed to optimize these conditions, as well as carbon and nitrogen sources, to enhance the mycelial growth of the white-rot fungus T....
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Academia.edu Journals
2024-08-01
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| Series: | Academia Green Energy |
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| author | Nikita Goyat Rajeev Kumar Kapoor Baljeet Singh Saharan Prexha Kapoor Deepika Dhanda |
| author_facet | Nikita Goyat Rajeev Kumar Kapoor Baljeet Singh Saharan Prexha Kapoor Deepika Dhanda |
| author_sort | Nikita Goyat |
| collection | DOAJ |
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Trametes versicolor, a common upper polyporus fungus, demonstrates mycelial growth that is influenced by temperature, pH, and media composition. This study aimed to optimize these conditions, as well as carbon and nitrogen sources, to enhance the mycelial growth of the white-rot fungus T. versicolor HBB 7328. This fungus shows significant potential for green energy applications due to its enzymatic activities. The research found that the fungus achieved maximal mycelial growth at 30°C (8.24 mm/day) and pH 7 (6.34 mm/day). Among various carbon sources, maltose resulted in the highest growth rate of 10.62 mm/day, whereas malt extract proved to be the most effective nitrogen source, supporting a growth rate of 8.96 mm/day. Enzymatic activity screening showed that T. versicolor HBB 7328 was positive for laccase, cellulase, protease, and xylanase activities. Notably, it exhibited substantial laccase activity (50.167 U/mL). These results are pivotal for developing pure cultures and conducting further studies on the enzymatic activities of T. versicolor HBB 7328. The high laccase activity, in particular, underscores its potential in biotechnological applications such as pollutant biodegradation and biofuel production, advancing green energy solutions. |
| format | Article |
| id | doaj-art-034eb46f0cb345e7b6b5a1e2168ddf15 |
| institution | Kabale University |
| issn | 2998-3665 |
| language | English |
| publishDate | 2024-08-01 |
| publisher | Academia.edu Journals |
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| series | Academia Green Energy |
| spelling | doaj-art-034eb46f0cb345e7b6b5a1e2168ddf152025-02-10T21:38:55ZengAcademia.edu JournalsAcademia Green Energy2998-36652024-08-011210.20935/AcadEnergy7306Optimizing mycelial growth of Trametes versicolor HBB 7328 for enzyme production and green energyNikita Goyat0Rajeev Kumar Kapoor1Baljeet Singh Saharan2Prexha Kapoor3Deepika Dhanda4Department of Microbiology, CCS Haryana Agricultural University, Hisar, Haryana 125 004, India.Department of Microbiology, Maharshi Dayanand University, Rohtak, Haryana 124 001, India.Department of Microbiology, CCS Haryana Agricultural University, Hisar, Haryana 125 004, India.Department of Molecular Biology, Biotechnology and Bioinformatics, CCS Haryana Agricultural University, Hisar, Haryana 125 004, India.Department of Botany and Plant Physiology (Environmental Science), CCS Haryana Agricultural University, Hisar, Haryana 125 004 India. Trametes versicolor, a common upper polyporus fungus, demonstrates mycelial growth that is influenced by temperature, pH, and media composition. This study aimed to optimize these conditions, as well as carbon and nitrogen sources, to enhance the mycelial growth of the white-rot fungus T. versicolor HBB 7328. This fungus shows significant potential for green energy applications due to its enzymatic activities. The research found that the fungus achieved maximal mycelial growth at 30°C (8.24 mm/day) and pH 7 (6.34 mm/day). Among various carbon sources, maltose resulted in the highest growth rate of 10.62 mm/day, whereas malt extract proved to be the most effective nitrogen source, supporting a growth rate of 8.96 mm/day. Enzymatic activity screening showed that T. versicolor HBB 7328 was positive for laccase, cellulase, protease, and xylanase activities. Notably, it exhibited substantial laccase activity (50.167 U/mL). These results are pivotal for developing pure cultures and conducting further studies on the enzymatic activities of T. versicolor HBB 7328. The high laccase activity, in particular, underscores its potential in biotechnological applications such as pollutant biodegradation and biofuel production, advancing green energy solutions.https://www.academia.edu/122930711/Optimizing_mycelial_growth_of_Trametes_versicolor_HBB_7328_for_enzyme_production_and_green_energy |
| spellingShingle | Nikita Goyat Rajeev Kumar Kapoor Baljeet Singh Saharan Prexha Kapoor Deepika Dhanda Optimizing mycelial growth of Trametes versicolor HBB 7328 for enzyme production and green energy Academia Green Energy |
| title | Optimizing mycelial growth of Trametes versicolor HBB 7328 for enzyme production and green energy |
| title_full | Optimizing mycelial growth of Trametes versicolor HBB 7328 for enzyme production and green energy |
| title_fullStr | Optimizing mycelial growth of Trametes versicolor HBB 7328 for enzyme production and green energy |
| title_full_unstemmed | Optimizing mycelial growth of Trametes versicolor HBB 7328 for enzyme production and green energy |
| title_short | Optimizing mycelial growth of Trametes versicolor HBB 7328 for enzyme production and green energy |
| title_sort | optimizing mycelial growth of trametes versicolor hbb 7328 for enzyme production and green energy |
| url | https://www.academia.edu/122930711/Optimizing_mycelial_growth_of_Trametes_versicolor_HBB_7328_for_enzyme_production_and_green_energy |
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