Effect of Surgical Installation of Dental Implants on Surface Topography and Its Influence on Osteoblast Proliferation

Surface treatment alone does not determine the final microtopography of a dental implant, which can be influenced by implant design and the surgical procedure. This study investigated the effect of surgical placement of dental implants with same surface treatments on surface roughness. Three implant...

Full description

Saved in:
Bibliographic Details
Main Authors: Helder H. M. Menezes, Marina M. Naves, Henara L. Costa, Tarsis P. Barbosa, Jéssica A. Ferreira, Denildo Magalhães, Elizabeth F. Martinez
Format: Article
Language:English
Published: Wiley 2018-01-01
Series:International Journal of Dentistry
Online Access:http://dx.doi.org/10.1155/2018/4089274
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850233342996250624
author Helder H. M. Menezes
Marina M. Naves
Henara L. Costa
Tarsis P. Barbosa
Jéssica A. Ferreira
Denildo Magalhães
Elizabeth F. Martinez
author_facet Helder H. M. Menezes
Marina M. Naves
Henara L. Costa
Tarsis P. Barbosa
Jéssica A. Ferreira
Denildo Magalhães
Elizabeth F. Martinez
author_sort Helder H. M. Menezes
collection DOAJ
description Surface treatment alone does not determine the final microtopography of a dental implant, which can be influenced by implant design and the surgical procedure. This study investigated the effect of surgical placement of dental implants with same surface treatments on surface roughness. Three implants (SIN) of each group with different macrogeometries (Strong, Stylus, and Tryon) were analyzed using laser interferometry and scanning electron microscopy to evaluate surface topography. All threaded regions of the implants, namely, top, flank, and valley, were analyzed individually. Relevant surface parameters (Sa, Ssk, Sku, Str, and Sdq) were calculated for the different regions on each implant before (B) (n = 9) and after (A) (n = 9) placement into porcine rib bones. The behavior and proliferation of a preosteoblastic cell line MC3T3-E1 on titanium surface, cell viability, and osteopontin secretion were evaluated after 24 h, 48 h, and 96 h, also before (n = 18) and after (n = 18) implant placement into porcine ribs bone. As results, the valleys of all implants had an increase in Sa values after implant placement. By contrast, the tops of the Stylus A implant and the flanks of the Tryon A implant showed a significant decrease in mean height of the irregularities (Sa), 0.16 µm and 1.25 µm, respectively. The Stylus implant presented significantly (p<0.05) higher asymmetry values on the distribution curve for irregularity heights (Sku) in all regions after insertion into bone (6.99 for tops, 9.54 for flanks, and 17.64 for valleys), indicating a greater preponderance of peaks over valleys. An increase in roughness gradients (Sdq) was observed for all macrogeometries after insertion into bone. The cell culture results showed no significant difference (p>0.05) for all macrogeometries after bone placement. In conclusion, a subtle change in implant surface roughness was detected after insertion into bone for all the macrogeometries, without significantly affecting the cellular parameters studied.
format Article
id doaj-art-034e3c67f4fa4a7581b14911bc53a366
institution OA Journals
issn 1687-8728
1687-8736
language English
publishDate 2018-01-01
publisher Wiley
record_format Article
series International Journal of Dentistry
spelling doaj-art-034e3c67f4fa4a7581b14911bc53a3662025-08-20T02:02:57ZengWileyInternational Journal of Dentistry1687-87281687-87362018-01-01201810.1155/2018/40892744089274Effect of Surgical Installation of Dental Implants on Surface Topography and Its Influence on Osteoblast ProliferationHelder H. M. Menezes0Marina M. Naves1Henara L. Costa2Tarsis P. Barbosa3Jéssica A. Ferreira4Denildo Magalhães5Elizabeth F. Martinez6Department of Periodontology and Implant Dentistry, HD Post Graduation Dental School, Uberlândia 38408-394, BrazilSchool of Dentistry, Federal University of Uberlandia, Uberlândia 38405-320, BrazilSchool of Mechanical Engineering, Federal University of Uberlandia, Uberlandia 38400-901, BrazilSchool of Mechanical Engineering, Federal University of Uberlandia, Uberlandia 38400-901, BrazilSchool of Dentistry, Federal University of Uberlandia, Uberlândia 38405-320, BrazilSchool of Dentistry, Federal University of Uberlandia, Uberlândia 38405-320, BrazilDepartment of Cellular and Mollecular Biology, São Leopoldo Mandic Research, Campinas 13045-755, BrazilSurface treatment alone does not determine the final microtopography of a dental implant, which can be influenced by implant design and the surgical procedure. This study investigated the effect of surgical placement of dental implants with same surface treatments on surface roughness. Three implants (SIN) of each group with different macrogeometries (Strong, Stylus, and Tryon) were analyzed using laser interferometry and scanning electron microscopy to evaluate surface topography. All threaded regions of the implants, namely, top, flank, and valley, were analyzed individually. Relevant surface parameters (Sa, Ssk, Sku, Str, and Sdq) were calculated for the different regions on each implant before (B) (n = 9) and after (A) (n = 9) placement into porcine rib bones. The behavior and proliferation of a preosteoblastic cell line MC3T3-E1 on titanium surface, cell viability, and osteopontin secretion were evaluated after 24 h, 48 h, and 96 h, also before (n = 18) and after (n = 18) implant placement into porcine ribs bone. As results, the valleys of all implants had an increase in Sa values after implant placement. By contrast, the tops of the Stylus A implant and the flanks of the Tryon A implant showed a significant decrease in mean height of the irregularities (Sa), 0.16 µm and 1.25 µm, respectively. The Stylus implant presented significantly (p<0.05) higher asymmetry values on the distribution curve for irregularity heights (Sku) in all regions after insertion into bone (6.99 for tops, 9.54 for flanks, and 17.64 for valleys), indicating a greater preponderance of peaks over valleys. An increase in roughness gradients (Sdq) was observed for all macrogeometries after insertion into bone. The cell culture results showed no significant difference (p>0.05) for all macrogeometries after bone placement. In conclusion, a subtle change in implant surface roughness was detected after insertion into bone for all the macrogeometries, without significantly affecting the cellular parameters studied.http://dx.doi.org/10.1155/2018/4089274
spellingShingle Helder H. M. Menezes
Marina M. Naves
Henara L. Costa
Tarsis P. Barbosa
Jéssica A. Ferreira
Denildo Magalhães
Elizabeth F. Martinez
Effect of Surgical Installation of Dental Implants on Surface Topography and Its Influence on Osteoblast Proliferation
International Journal of Dentistry
title Effect of Surgical Installation of Dental Implants on Surface Topography and Its Influence on Osteoblast Proliferation
title_full Effect of Surgical Installation of Dental Implants on Surface Topography and Its Influence on Osteoblast Proliferation
title_fullStr Effect of Surgical Installation of Dental Implants on Surface Topography and Its Influence on Osteoblast Proliferation
title_full_unstemmed Effect of Surgical Installation of Dental Implants on Surface Topography and Its Influence on Osteoblast Proliferation
title_short Effect of Surgical Installation of Dental Implants on Surface Topography and Its Influence on Osteoblast Proliferation
title_sort effect of surgical installation of dental implants on surface topography and its influence on osteoblast proliferation
url http://dx.doi.org/10.1155/2018/4089274
work_keys_str_mv AT helderhmmenezes effectofsurgicalinstallationofdentalimplantsonsurfacetopographyanditsinfluenceonosteoblastproliferation
AT marinamnaves effectofsurgicalinstallationofdentalimplantsonsurfacetopographyanditsinfluenceonosteoblastproliferation
AT henaralcosta effectofsurgicalinstallationofdentalimplantsonsurfacetopographyanditsinfluenceonosteoblastproliferation
AT tarsispbarbosa effectofsurgicalinstallationofdentalimplantsonsurfacetopographyanditsinfluenceonosteoblastproliferation
AT jessicaaferreira effectofsurgicalinstallationofdentalimplantsonsurfacetopographyanditsinfluenceonosteoblastproliferation
AT denildomagalhaes effectofsurgicalinstallationofdentalimplantsonsurfacetopographyanditsinfluenceonosteoblastproliferation
AT elizabethfmartinez effectofsurgicalinstallationofdentalimplantsonsurfacetopographyanditsinfluenceonosteoblastproliferation