Phyllospongia foliascens-Derived Fungi: Molecular Identification, Antimicrobial Activity and Secondary Metabolite Profile of Their Methanolic Extracts
Sponge-derived fungi are considered a promising source for lead compounds in drug discovery. This study aimed to identify fungi isolated from the sponge Phyllospongia foliascens collected in Amed, Bali, Indonesia, and investigate the antimicrobial activity and secondary metabolites of their methanol...
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EDP Sciences
2025-01-01
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| Series: | BIO Web of Conferences |
| Online Access: | https://www.bio-conferences.org/articles/bioconf/pdf/2025/27/bioconf_inflection2025_02003.pdf |
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| author | Putra I Putu Yogi Astara Ariantari Ni Putu Wibowo Joko Tri Leliqia Ni Putu Eka Dwija Ida Bagus Nyoman Putra Wulandari Ni Made Widya Dwijayanti Ni Kadek Putri Ni Putu Ananda Eka Dewi Komang Tria Noviana |
| author_facet | Putra I Putu Yogi Astara Ariantari Ni Putu Wibowo Joko Tri Leliqia Ni Putu Eka Dwija Ida Bagus Nyoman Putra Wulandari Ni Made Widya Dwijayanti Ni Kadek Putri Ni Putu Ananda Eka Dewi Komang Tria Noviana |
| author_sort | Putra I Putu Yogi Astara |
| collection | DOAJ |
| description | Sponge-derived fungi are considered a promising source for lead compounds in drug discovery. This study aimed to identify fungi isolated from the sponge Phyllospongia foliascens collected in Amed, Bali, Indonesia, and investigate the antimicrobial activity and secondary metabolites of their methanolic extracts. The identification of pure fungal isolates was done through molecular biology procedure. After rice fermentation of the pure fungal isolates, extraction was carried out using ethyl acetate. The crude extract was subjected to liquid-liquid separation between n-hexane and aqueous methanol. The resulting methanolic phase was dried in vacuo and used for further analysis and antimicrobial assay. Five fungal strains isolated in this study were identified as Aspergillus nomiae SP 1-1-1, Penicillium steckii SP 1-1-2, Penicillium citrinum SP 1-2-4-1, Aspergillus niger SP 1-2-4-2, and Aspergillus sydowii RM-NS-SP-1 (B). Alkaloids were detected in all fungal extracts, while flavonoids and polyphenols were detected in some extracts. Antimicrobial tests employing the disk diffusion method exhibited that P. citrinum SP 1-2-4-1 extract obtained from its fermentation on rice media without salt had pronounced activity against Streptococcus mutans ATCC 35668 with an inhibition zone of 9.61 mm. Meanwhile, the extract afforded from the rice cultures of A. sydowii RM-NS-SP-1 (B) with the addition of salt was active against Staphylococcus aureus ATCC 25923, Methicillin-resistant S. aureus ATCC 3351, and Staphylococcus epidermidis ATCC 12228 with zone inhibitions of 8.27, 2.53, and 5.37 mm, respectively. Thin-layer chromatography-densitometry analysis of both extracts revealed different metabolite profiles, where many peaks were suppressed in the fungal extract obtained from the salt-containing culture. |
| format | Article |
| id | doaj-art-034d75499c7e48869d38e16f24ba24a5 |
| institution | Kabale University |
| issn | 2117-4458 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | BIO Web of Conferences |
| spelling | doaj-art-034d75499c7e48869d38e16f24ba24a52025-08-20T03:47:03ZengEDP SciencesBIO Web of Conferences2117-44582025-01-011760200310.1051/bioconf/202517602003bioconf_inflection2025_02003Phyllospongia foliascens-Derived Fungi: Molecular Identification, Antimicrobial Activity and Secondary Metabolite Profile of Their Methanolic ExtractsPutra I Putu Yogi Astara0Ariantari Ni Putu1Wibowo Joko Tri2Leliqia Ni Putu Eka3Dwija Ida Bagus Nyoman Putra4Wulandari Ni Made Widya5Dwijayanti Ni Kadek6Putri Ni Putu Ananda Eka7Dewi Komang Tria Noviana8Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Udayana UniversityDepartment of Pharmacy, Faculty of Mathematics and Natural Sciences, Udayana UniversityResearch Center for Vaccine and Drugs, National Research and Innovation Agency of Indonesia (BRIN)Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Udayana UniversityDepartment of Microbiology, Faculty of Medicine, Udayana UniversityDepartment of Pharmacy, Faculty of Mathematics and Natural Sciences, Udayana UniversityDepartment of Pharmacy, Faculty of Mathematics and Natural Sciences, Udayana UniversityDepartment of Pharmacy, Faculty of Mathematics and Natural Sciences, Udayana UniversityDepartment of Pharmacy, Faculty of Mathematics and Natural Sciences, Udayana UniversitySponge-derived fungi are considered a promising source for lead compounds in drug discovery. This study aimed to identify fungi isolated from the sponge Phyllospongia foliascens collected in Amed, Bali, Indonesia, and investigate the antimicrobial activity and secondary metabolites of their methanolic extracts. The identification of pure fungal isolates was done through molecular biology procedure. After rice fermentation of the pure fungal isolates, extraction was carried out using ethyl acetate. The crude extract was subjected to liquid-liquid separation between n-hexane and aqueous methanol. The resulting methanolic phase was dried in vacuo and used for further analysis and antimicrobial assay. Five fungal strains isolated in this study were identified as Aspergillus nomiae SP 1-1-1, Penicillium steckii SP 1-1-2, Penicillium citrinum SP 1-2-4-1, Aspergillus niger SP 1-2-4-2, and Aspergillus sydowii RM-NS-SP-1 (B). Alkaloids were detected in all fungal extracts, while flavonoids and polyphenols were detected in some extracts. Antimicrobial tests employing the disk diffusion method exhibited that P. citrinum SP 1-2-4-1 extract obtained from its fermentation on rice media without salt had pronounced activity against Streptococcus mutans ATCC 35668 with an inhibition zone of 9.61 mm. Meanwhile, the extract afforded from the rice cultures of A. sydowii RM-NS-SP-1 (B) with the addition of salt was active against Staphylococcus aureus ATCC 25923, Methicillin-resistant S. aureus ATCC 3351, and Staphylococcus epidermidis ATCC 12228 with zone inhibitions of 8.27, 2.53, and 5.37 mm, respectively. Thin-layer chromatography-densitometry analysis of both extracts revealed different metabolite profiles, where many peaks were suppressed in the fungal extract obtained from the salt-containing culture.https://www.bio-conferences.org/articles/bioconf/pdf/2025/27/bioconf_inflection2025_02003.pdf |
| spellingShingle | Putra I Putu Yogi Astara Ariantari Ni Putu Wibowo Joko Tri Leliqia Ni Putu Eka Dwija Ida Bagus Nyoman Putra Wulandari Ni Made Widya Dwijayanti Ni Kadek Putri Ni Putu Ananda Eka Dewi Komang Tria Noviana Phyllospongia foliascens-Derived Fungi: Molecular Identification, Antimicrobial Activity and Secondary Metabolite Profile of Their Methanolic Extracts BIO Web of Conferences |
| title | Phyllospongia foliascens-Derived Fungi: Molecular Identification, Antimicrobial Activity and Secondary Metabolite Profile of Their Methanolic Extracts |
| title_full | Phyllospongia foliascens-Derived Fungi: Molecular Identification, Antimicrobial Activity and Secondary Metabolite Profile of Their Methanolic Extracts |
| title_fullStr | Phyllospongia foliascens-Derived Fungi: Molecular Identification, Antimicrobial Activity and Secondary Metabolite Profile of Their Methanolic Extracts |
| title_full_unstemmed | Phyllospongia foliascens-Derived Fungi: Molecular Identification, Antimicrobial Activity and Secondary Metabolite Profile of Their Methanolic Extracts |
| title_short | Phyllospongia foliascens-Derived Fungi: Molecular Identification, Antimicrobial Activity and Secondary Metabolite Profile of Their Methanolic Extracts |
| title_sort | phyllospongia foliascens derived fungi molecular identification antimicrobial activity and secondary metabolite profile of their methanolic extracts |
| url | https://www.bio-conferences.org/articles/bioconf/pdf/2025/27/bioconf_inflection2025_02003.pdf |
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