The Anticancer Activity of Cetraria Islandica (L.) Ach in Breast Cancer Cells Through Crosstalk of Ampk-α1 and Erk1/2 Signalling
In the present study, we aimed to evaluate the anticancer activities of Cetraria islandica (C.islandica) extracts on MCF-7 breast cancer cell lines. Cell viability, protein levels, apoptotic cells number, F-actin distribution were measured. Cell viability of MCF-7 breast cancer cells was found to be...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Hasan Eleroğlu
2018-06-01
|
| Series: | Turkish Journal of Agriculture: Food Science and Technology |
| Subjects: | |
| Online Access: | http://www.agrifoodscience.com/index.php/TURJAF/article/view/1940 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849704472326963200 |
|---|---|
| author | Celal Güven Eylem Taskın Onder Yumtutas Leyla Turker Sener Yusuf Ozay Fulya Dal Mufide Ahbab Ibrahim Bozgeyik Isil Albeniz Haydar Bagıs Atilla Yıldız |
| author_facet | Celal Güven Eylem Taskın Onder Yumtutas Leyla Turker Sener Yusuf Ozay Fulya Dal Mufide Ahbab Ibrahim Bozgeyik Isil Albeniz Haydar Bagıs Atilla Yıldız |
| author_sort | Celal Güven |
| collection | DOAJ |
| description | In the present study, we aimed to evaluate the anticancer activities of Cetraria islandica (C.islandica) extracts on MCF-7 breast cancer cell lines. Cell viability, protein levels, apoptotic cells number, F-actin distribution were measured. Cell viability of MCF-7 breast cancer cells was found to be reduced in a dose-dependent manner.EC50 values of C.islandica on MCF-7 cells were found to be 9.2047 E-5 g/ml (cell amount) by using intelligence system. Expressions of p53, caspase 3 and Bcl-2, were shown to be elevated after low doses of extract and diminished after high dose treatments. PPAR- protein level was decreased, although AMP-activated kinases-α1 (AMPK-α1) protein level was increasedin its extract groups. ERK1/2 protein level was also elevated in its extract groups. 125 mg/ml of extract treated cells show a low decrease in actin filament density. MCF-7 cells with C.islandica treatment for 24 h increased the apoptotic cell percentage, though the cells-treated with C.islandica for 48 was high necrotic cells percentage. Consequently, the C.islandica extract treatment causes to elevate ERK1/2 and AMPK-α1 protein levels, resulting in PPAR- and then triggers the apoptosis by modulation caspase-3 and P53 protein levels. Therefore, C.islandica might be a good candidate for anticancer tissue, especially soft tissue tumours. |
| format | Article |
| id | doaj-art-033ab558ccde47a7a303e89e010351d0 |
| institution | DOAJ |
| issn | 2148-127X |
| language | English |
| publishDate | 2018-06-01 |
| publisher | Hasan Eleroğlu |
| record_format | Article |
| series | Turkish Journal of Agriculture: Food Science and Technology |
| spelling | doaj-art-033ab558ccde47a7a303e89e010351d02025-08-20T03:16:46ZengHasan EleroğluTurkish Journal of Agriculture: Food Science and Technology2148-127X2018-06-016678379110.24925/turjaf.v6i6.783-791.1940876The Anticancer Activity of Cetraria Islandica (L.) Ach in Breast Cancer Cells Through Crosstalk of Ampk-α1 and Erk1/2 SignallingCelal Güven0Eylem Taskın1Onder Yumtutas2Leyla Turker Sener3Yusuf Ozay4Fulya Dal5Mufide Ahbab6Ibrahim Bozgeyik7Isil Albeniz8Haydar Bagıs9Atilla Yıldız10Department of Biophysics, Faculty of Medicine, Omer Halisdemir University, NigdeDepartment of Physiology, Faculty of Medicine, Nigde Omer Halisdemir University, Turkey Department of Biophysics, Faculty of Medicine, Cukurova UniversityDepartment of Medical Biology, Faculty of Medicine, Adiyaman University, AdiyamanDepartment of Biophysics, Faculty of Medicine, Istanbul University, IstanbulDepartment of Medical Biology, Faculty of Medicine, Adiyaman University, AdiyamanDepartment of Biophysics, Faculty of Medicine, Halic University, IstanbulDepartment of Biology, Faculty of Science, Ankara University, AnkaraDepartment of Medical Biology, Faculty of Medicine, Adiyaman University, AdiyamanDepartment of Biophysics, Faculty of Medicine, Istanbul University, Istanbul, TurkeyDepartment of Medical Genetics, Faculty of Medicine, Adiyaman University, Adiyaman9Department of Biology, Faculty of Science, Ankara University, AnkaraIn the present study, we aimed to evaluate the anticancer activities of Cetraria islandica (C.islandica) extracts on MCF-7 breast cancer cell lines. Cell viability, protein levels, apoptotic cells number, F-actin distribution were measured. Cell viability of MCF-7 breast cancer cells was found to be reduced in a dose-dependent manner.EC50 values of C.islandica on MCF-7 cells were found to be 9.2047 E-5 g/ml (cell amount) by using intelligence system. Expressions of p53, caspase 3 and Bcl-2, were shown to be elevated after low doses of extract and diminished after high dose treatments. PPAR- protein level was decreased, although AMP-activated kinases-α1 (AMPK-α1) protein level was increasedin its extract groups. ERK1/2 protein level was also elevated in its extract groups. 125 mg/ml of extract treated cells show a low decrease in actin filament density. MCF-7 cells with C.islandica treatment for 24 h increased the apoptotic cell percentage, though the cells-treated with C.islandica for 48 was high necrotic cells percentage. Consequently, the C.islandica extract treatment causes to elevate ERK1/2 and AMPK-α1 protein levels, resulting in PPAR- and then triggers the apoptosis by modulation caspase-3 and P53 protein levels. Therefore, C.islandica might be a good candidate for anticancer tissue, especially soft tissue tumours.http://www.agrifoodscience.com/index.php/TURJAF/article/view/1940Cetraria islandicaAnticancerAntiproliferationAMPKAntiapoptoticMitogen-activated kinasesPhytotherapyPPAR |
| spellingShingle | Celal Güven Eylem Taskın Onder Yumtutas Leyla Turker Sener Yusuf Ozay Fulya Dal Mufide Ahbab Ibrahim Bozgeyik Isil Albeniz Haydar Bagıs Atilla Yıldız The Anticancer Activity of Cetraria Islandica (L.) Ach in Breast Cancer Cells Through Crosstalk of Ampk-α1 and Erk1/2 Signalling Turkish Journal of Agriculture: Food Science and Technology Cetraria islandica Anticancer Antiproliferation AMPK Antiapoptotic Mitogen-activated kinases Phytotherapy PPAR |
| title | The Anticancer Activity of Cetraria Islandica (L.) Ach in Breast Cancer Cells Through Crosstalk of Ampk-α1 and Erk1/2 Signalling |
| title_full | The Anticancer Activity of Cetraria Islandica (L.) Ach in Breast Cancer Cells Through Crosstalk of Ampk-α1 and Erk1/2 Signalling |
| title_fullStr | The Anticancer Activity of Cetraria Islandica (L.) Ach in Breast Cancer Cells Through Crosstalk of Ampk-α1 and Erk1/2 Signalling |
| title_full_unstemmed | The Anticancer Activity of Cetraria Islandica (L.) Ach in Breast Cancer Cells Through Crosstalk of Ampk-α1 and Erk1/2 Signalling |
| title_short | The Anticancer Activity of Cetraria Islandica (L.) Ach in Breast Cancer Cells Through Crosstalk of Ampk-α1 and Erk1/2 Signalling |
| title_sort | anticancer activity of cetraria islandica l ach in breast cancer cells through crosstalk of ampk α1 and erk1 2 signalling |
| topic | Cetraria islandica Anticancer Antiproliferation AMPK Antiapoptotic Mitogen-activated kinases Phytotherapy PPAR |
| url | http://www.agrifoodscience.com/index.php/TURJAF/article/view/1940 |
| work_keys_str_mv | AT celalguven theanticanceractivityofcetrariaislandicalachinbreastcancercellsthroughcrosstalkofampka1anderk12signalling AT eylemtaskın theanticanceractivityofcetrariaislandicalachinbreastcancercellsthroughcrosstalkofampka1anderk12signalling AT onderyumtutas theanticanceractivityofcetrariaislandicalachinbreastcancercellsthroughcrosstalkofampka1anderk12signalling AT leylaturkersener theanticanceractivityofcetrariaislandicalachinbreastcancercellsthroughcrosstalkofampka1anderk12signalling AT yusufozay theanticanceractivityofcetrariaislandicalachinbreastcancercellsthroughcrosstalkofampka1anderk12signalling AT fulyadal theanticanceractivityofcetrariaislandicalachinbreastcancercellsthroughcrosstalkofampka1anderk12signalling AT mufideahbab theanticanceractivityofcetrariaislandicalachinbreastcancercellsthroughcrosstalkofampka1anderk12signalling AT ibrahimbozgeyik theanticanceractivityofcetrariaislandicalachinbreastcancercellsthroughcrosstalkofampka1anderk12signalling AT isilalbeniz theanticanceractivityofcetrariaislandicalachinbreastcancercellsthroughcrosstalkofampka1anderk12signalling AT haydarbagıs theanticanceractivityofcetrariaislandicalachinbreastcancercellsthroughcrosstalkofampka1anderk12signalling AT atillayıldız theanticanceractivityofcetrariaislandicalachinbreastcancercellsthroughcrosstalkofampka1anderk12signalling AT celalguven anticanceractivityofcetrariaislandicalachinbreastcancercellsthroughcrosstalkofampka1anderk12signalling AT eylemtaskın anticanceractivityofcetrariaislandicalachinbreastcancercellsthroughcrosstalkofampka1anderk12signalling AT onderyumtutas anticanceractivityofcetrariaislandicalachinbreastcancercellsthroughcrosstalkofampka1anderk12signalling AT leylaturkersener anticanceractivityofcetrariaislandicalachinbreastcancercellsthroughcrosstalkofampka1anderk12signalling AT yusufozay anticanceractivityofcetrariaislandicalachinbreastcancercellsthroughcrosstalkofampka1anderk12signalling AT fulyadal anticanceractivityofcetrariaislandicalachinbreastcancercellsthroughcrosstalkofampka1anderk12signalling AT mufideahbab anticanceractivityofcetrariaislandicalachinbreastcancercellsthroughcrosstalkofampka1anderk12signalling AT ibrahimbozgeyik anticanceractivityofcetrariaislandicalachinbreastcancercellsthroughcrosstalkofampka1anderk12signalling AT isilalbeniz anticanceractivityofcetrariaislandicalachinbreastcancercellsthroughcrosstalkofampka1anderk12signalling AT haydarbagıs anticanceractivityofcetrariaislandicalachinbreastcancercellsthroughcrosstalkofampka1anderk12signalling AT atillayıldız anticanceractivityofcetrariaislandicalachinbreastcancercellsthroughcrosstalkofampka1anderk12signalling |