Mapping the key players in Kawasaki disease; role of inflammatory genes and protein-protein interactions
Background: Kawasaki disease (KD) is a complex acquired condition characterized by systemic blood vessel inflammation that primarily affects children under five years of age. It is clinically diagnosed as a syndrome, making it susceptible to misdiagnoses. Severe complications such as myocardial dama...
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Elsevier
2025-01-01
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| Series: | Informatics in Medicine Unlocked |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352914825000334 |
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| author | Wael Hafez Feras Al-Obeidat Asrar Rashid Afsheen Raza Nouran Hamza Nesma Ahmed Marwa M. Abdeljawad Raziya Kadwa Abdelhameed Elmesery Muneir Gador Dina Khair Gihan Zina fatema Abdulaal Mina Wassef Girgiss Maha Abdelhadi Ahmed Abdelrahman Mahmad Anwar Ibrahim Mohamed El Sherbiny |
| author_facet | Wael Hafez Feras Al-Obeidat Asrar Rashid Afsheen Raza Nouran Hamza Nesma Ahmed Marwa M. Abdeljawad Raziya Kadwa Abdelhameed Elmesery Muneir Gador Dina Khair Gihan Zina fatema Abdulaal Mina Wassef Girgiss Maha Abdelhadi Ahmed Abdelrahman Mahmad Anwar Ibrahim Mohamed El Sherbiny |
| author_sort | Wael Hafez |
| collection | DOAJ |
| description | Background: Kawasaki disease (KD) is a complex acquired condition characterized by systemic blood vessel inflammation that primarily affects children under five years of age. It is clinically diagnosed as a syndrome, making it susceptible to misdiagnoses. Severe complications such as myocardial damage and coronary artery abnormalities can be fatal; thus, early diagnosis is critical for preventing disease progression. Currently, no specific diagnostic test can distinguish KD from viral or bacterial infections. Additionally, the molecular mechanisms underlying the disease remain unclear, hindering the development of targeted therapies. Objective: This study aimed to identify the genetic patterns and molecular mechanisms associated with KD using a comprehensive gene expression analysis. Methods: RNA sequencing and microarray genomic datasets were retrieved from the NCBI Gene Expression Omnibus (GEO). Four datasets (GSE68004, GSE63881, GSE73461, and GSE73463) were used for the final analysis. These datasets compared patients with KD to healthy controls, and patients with acute KD to convalescent patients. Differentially expressed genes (DEGs) were identified in the datasets. Enrichment analysis was conducted, followed by protein-protein interaction (PPI) network analysis to identify hub genes. Heatmaps were generated to visualize gene expression patterns. Results: Eighteen hub genes were identified in the KD versus control comparison, whereas 20 hub genes were identified in the acute versus convalescent analysis. These genes play key roles in inflammation, cytokine storm, innate immune modulation, and endothelial damage. Conclusion: This study provides valuable insights into the molecular mechanisms underlying KD, and identifies potential diagnostic biomarkers and therapeutic targets. |
| format | Article |
| id | doaj-art-033a8d0cedd94554a1477dbab72cf595 |
| institution | OA Journals |
| issn | 2352-9148 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Informatics in Medicine Unlocked |
| spelling | doaj-art-033a8d0cedd94554a1477dbab72cf5952025-08-20T02:33:55ZengElsevierInformatics in Medicine Unlocked2352-91482025-01-015610164510.1016/j.imu.2025.101645Mapping the key players in Kawasaki disease; role of inflammatory genes and protein-protein interactionsWael Hafez0Feras Al-Obeidat1Asrar Rashid2Afsheen Raza3Nouran Hamza4Nesma Ahmed5Marwa M. Abdeljawad6Raziya Kadwa7Abdelhameed Elmesery8Muneir Gador9Dina Khair10Gihan Zina11fatema Abdulaal12Mina Wassef Girgiss13Maha Abdelhadi14Ahmed Abdelrahman15Mahmad Anwar Ibrahim16Mohamed El Sherbiny17Medical Research and Clinical Studies Institute, The National Research Centre, Cairo, Egypt; NMC Royal Hospital, Khalifa City, Abu Dhabi, United Arab Emirates; The Emirates Society of Internal Medicine (ESIM), Emirates Medical Association, 46th Street Deira Near International Bowling Centre - Dubai info@ema.ae United Arab EmiratesCollege of Technological Innovation, Zayed University, Abu Dhabi, United Arab Emirates; Corresponding author. College of Technological Innovation at Zayed University, Abu Dhabi, United Arab Emirates.Edinburgh Napier University, Department of Computer Science, Merchiston Campus, 10 Colinton Road, Edinburgh, EH10 5DT, Scotland, UKMolecular Biology, College of Health Science, Abu Dhabi University, Abu Dhabi, United Arab EmiratesEMS Research and Development, Dubai, United Arab EmiratesEMS Research and Development, Dubai, United Arab EmiratesEMS Research and Development, Dubai, United Arab EmiratesNMC Royal Hospital, Khalifa City, Abu Dhabi, United Arab EmiratesNMC Royal Hospital, Khalifa City, Abu Dhabi, United Arab EmiratesNMC Royal Hospital, Khalifa City, Abu Dhabi, United Arab EmiratesNMC Royal Hospital, Khalifa City, Abu Dhabi, United Arab EmiratesNMC Royal Hospital, Khalifa City, Abu Dhabi, United Arab EmiratesNMC Royal Hospital, Khalifa City, Abu Dhabi, United Arab EmiratesMedical Research and Clinical Studies Institute, The National Research Centre, Cairo, EgyptMedical Research and Clinical Studies Institute, The National Research Centre, Cairo, EgyptInternal Medicine Department, Zagazig Faculty of Medicine, Zagazig, EgyptMediclinic Airport Hospital, Abu Dhabi, United Arab EmiratesInternal Medicine Department, Kasr Elaini School of Medicine, Cairo University, Cairo, EgyptBackground: Kawasaki disease (KD) is a complex acquired condition characterized by systemic blood vessel inflammation that primarily affects children under five years of age. It is clinically diagnosed as a syndrome, making it susceptible to misdiagnoses. Severe complications such as myocardial damage and coronary artery abnormalities can be fatal; thus, early diagnosis is critical for preventing disease progression. Currently, no specific diagnostic test can distinguish KD from viral or bacterial infections. Additionally, the molecular mechanisms underlying the disease remain unclear, hindering the development of targeted therapies. Objective: This study aimed to identify the genetic patterns and molecular mechanisms associated with KD using a comprehensive gene expression analysis. Methods: RNA sequencing and microarray genomic datasets were retrieved from the NCBI Gene Expression Omnibus (GEO). Four datasets (GSE68004, GSE63881, GSE73461, and GSE73463) were used for the final analysis. These datasets compared patients with KD to healthy controls, and patients with acute KD to convalescent patients. Differentially expressed genes (DEGs) were identified in the datasets. Enrichment analysis was conducted, followed by protein-protein interaction (PPI) network analysis to identify hub genes. Heatmaps were generated to visualize gene expression patterns. Results: Eighteen hub genes were identified in the KD versus control comparison, whereas 20 hub genes were identified in the acute versus convalescent analysis. These genes play key roles in inflammation, cytokine storm, innate immune modulation, and endothelial damage. Conclusion: This study provides valuable insights into the molecular mechanisms underlying KD, and identifies potential diagnostic biomarkers and therapeutic targets.http://www.sciencedirect.com/science/article/pii/S2352914825000334Kawasaki diseaseGene expression analysisInflammation biomarkersProtein-protein interactionCytokine regulation |
| spellingShingle | Wael Hafez Feras Al-Obeidat Asrar Rashid Afsheen Raza Nouran Hamza Nesma Ahmed Marwa M. Abdeljawad Raziya Kadwa Abdelhameed Elmesery Muneir Gador Dina Khair Gihan Zina fatema Abdulaal Mina Wassef Girgiss Maha Abdelhadi Ahmed Abdelrahman Mahmad Anwar Ibrahim Mohamed El Sherbiny Mapping the key players in Kawasaki disease; role of inflammatory genes and protein-protein interactions Informatics in Medicine Unlocked Kawasaki disease Gene expression analysis Inflammation biomarkers Protein-protein interaction Cytokine regulation |
| title | Mapping the key players in Kawasaki disease; role of inflammatory genes and protein-protein interactions |
| title_full | Mapping the key players in Kawasaki disease; role of inflammatory genes and protein-protein interactions |
| title_fullStr | Mapping the key players in Kawasaki disease; role of inflammatory genes and protein-protein interactions |
| title_full_unstemmed | Mapping the key players in Kawasaki disease; role of inflammatory genes and protein-protein interactions |
| title_short | Mapping the key players in Kawasaki disease; role of inflammatory genes and protein-protein interactions |
| title_sort | mapping the key players in kawasaki disease role of inflammatory genes and protein protein interactions |
| topic | Kawasaki disease Gene expression analysis Inflammation biomarkers Protein-protein interaction Cytokine regulation |
| url | http://www.sciencedirect.com/science/article/pii/S2352914825000334 |
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