Accurate Delivery of Mesenchymal Stem Cell Spheroids With Platelet‐Rich Fibrin Shield: Enhancing Survival and Repair Functions of Sp‐MSCs in Diabetic Wound Healing
Abstract Diabetic wound is a significant clinical challenge, and stem cell therapy has shown great potential. This study explores the role of mesenchymal stem cell (MSC) spheroids (Sp‐MSCs) in healing diabetic wounds and the use of autologous plasma‐rich platelet fibrin (PRF) as a scaffold for Sp‐MS...
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| Format: | Article |
| Language: | English |
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Wiley
2025-07-01
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| Series: | Advanced Science |
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| Online Access: | https://doi.org/10.1002/advs.202413430 |
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| author | Jinglve Zhang Wenqing Xu Yutian Xiao Dingheng Su Yusheng He Huohong Yang Yixin Xie Xiaofang Wang Ren‐He Xu Shaorong Lei Dingyu Wu |
| author_facet | Jinglve Zhang Wenqing Xu Yutian Xiao Dingheng Su Yusheng He Huohong Yang Yixin Xie Xiaofang Wang Ren‐He Xu Shaorong Lei Dingyu Wu |
| author_sort | Jinglve Zhang |
| collection | DOAJ |
| description | Abstract Diabetic wound is a significant clinical challenge, and stem cell therapy has shown great potential. This study explores the role of mesenchymal stem cell (MSC) spheroids (Sp‐MSCs) in healing diabetic wounds and the use of autologous plasma‐rich platelet fibrin (PRF) as a scaffold for Sp‐MSCs. Through activation of the coagulation system, PRF offers a protective fibrin shield for Sp‐MSCs to promote the rapid recovery migration and proliferation of MSCs while maintaining the activity of Sp‐MSCs in an inflammatory overload environment by activating the related genes of Integrin‐β1‐vascular endothelial growth factor (VEGF), and Wnt/β‐catenin pathways. The inclusion of Sp‐MSCs accelerates the gelation of PRF and results in improved mechanical strength. Additionally, PRF enhances the repair function of Sp‐MSCs, creating a favorable microenvironment for angiogenesis. In the wound model of diabetic mice, the combination of PRF with Sp‐MSCs accelerates wound healing. Results show that this combination significantly promotes wound repair and regulates the immune microenvironment. The study suggests that PRF is a promising bio‐derived scaffold for stem cell applications in diabetic wounds, offering new directions for stem cell therapy and biomimetic scaffold material development. |
| format | Article |
| id | doaj-art-033983943f3847c19df8215f1cb35d52 |
| institution | Kabale University |
| issn | 2198-3844 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj-art-033983943f3847c19df8215f1cb35d522025-08-20T03:28:58ZengWileyAdvanced Science2198-38442025-07-011225n/an/a10.1002/advs.202413430Accurate Delivery of Mesenchymal Stem Cell Spheroids With Platelet‐Rich Fibrin Shield: Enhancing Survival and Repair Functions of Sp‐MSCs in Diabetic Wound HealingJinglve Zhang0Wenqing Xu1Yutian Xiao2Dingheng Su3Yusheng He4Huohong Yang5Yixin Xie6Xiaofang Wang7Ren‐He Xu8Shaorong Lei9Dingyu Wu10Department of Plastic Surgery Xiangya Hospital Central South University 87 Xiangya Road Changsha Hunan 410008 ChinaDepartment of Plastic Surgery Xiangya Hospital Central South University 87 Xiangya Road Changsha Hunan 410008 ChinaDepartment of Plastic Surgery Xiangya Hospital Central South University 87 Xiangya Road Changsha Hunan 410008 ChinaDepartment of Plastic Surgery Xiangya Hospital Central South University 87 Xiangya Road Changsha Hunan 410008 ChinaImStem Biotechnology, Inc. 400 Farmington Avenue R1808 Farmington CT 06030 USAImStem Biotechnology, Inc. 400 Farmington Avenue R1808 Farmington CT 06030 USAImStem Biotechnology, Inc. 400 Farmington Avenue R1808 Farmington CT 06030 USAImStem Biotechnology, Inc. 400 Farmington Avenue R1808 Farmington CT 06030 USAMinistry of Education Frontiers Science Center for Precision Oncology Center of Reproduction Development & Aging and Institute of Translational Medicine Faculty of Health Sciences University of Macau Taipa Macau ChinaDepartment of Plastic Surgery Xiangya Hospital Central South University 87 Xiangya Road Changsha Hunan 410008 ChinaDepartment of Plastic Surgery Xiangya Hospital Central South University 87 Xiangya Road Changsha Hunan 410008 ChinaAbstract Diabetic wound is a significant clinical challenge, and stem cell therapy has shown great potential. This study explores the role of mesenchymal stem cell (MSC) spheroids (Sp‐MSCs) in healing diabetic wounds and the use of autologous plasma‐rich platelet fibrin (PRF) as a scaffold for Sp‐MSCs. Through activation of the coagulation system, PRF offers a protective fibrin shield for Sp‐MSCs to promote the rapid recovery migration and proliferation of MSCs while maintaining the activity of Sp‐MSCs in an inflammatory overload environment by activating the related genes of Integrin‐β1‐vascular endothelial growth factor (VEGF), and Wnt/β‐catenin pathways. The inclusion of Sp‐MSCs accelerates the gelation of PRF and results in improved mechanical strength. Additionally, PRF enhances the repair function of Sp‐MSCs, creating a favorable microenvironment for angiogenesis. In the wound model of diabetic mice, the combination of PRF with Sp‐MSCs accelerates wound healing. Results show that this combination significantly promotes wound repair and regulates the immune microenvironment. The study suggests that PRF is a promising bio‐derived scaffold for stem cell applications in diabetic wounds, offering new directions for stem cell therapy and biomimetic scaffold material development.https://doi.org/10.1002/advs.202413430cell spheroiddiabetic woundmesenchymal stem cellplatelet‐rich fiberROS protection |
| spellingShingle | Jinglve Zhang Wenqing Xu Yutian Xiao Dingheng Su Yusheng He Huohong Yang Yixin Xie Xiaofang Wang Ren‐He Xu Shaorong Lei Dingyu Wu Accurate Delivery of Mesenchymal Stem Cell Spheroids With Platelet‐Rich Fibrin Shield: Enhancing Survival and Repair Functions of Sp‐MSCs in Diabetic Wound Healing Advanced Science cell spheroid diabetic wound mesenchymal stem cell platelet‐rich fiber ROS protection |
| title | Accurate Delivery of Mesenchymal Stem Cell Spheroids With Platelet‐Rich Fibrin Shield: Enhancing Survival and Repair Functions of Sp‐MSCs in Diabetic Wound Healing |
| title_full | Accurate Delivery of Mesenchymal Stem Cell Spheroids With Platelet‐Rich Fibrin Shield: Enhancing Survival and Repair Functions of Sp‐MSCs in Diabetic Wound Healing |
| title_fullStr | Accurate Delivery of Mesenchymal Stem Cell Spheroids With Platelet‐Rich Fibrin Shield: Enhancing Survival and Repair Functions of Sp‐MSCs in Diabetic Wound Healing |
| title_full_unstemmed | Accurate Delivery of Mesenchymal Stem Cell Spheroids With Platelet‐Rich Fibrin Shield: Enhancing Survival and Repair Functions of Sp‐MSCs in Diabetic Wound Healing |
| title_short | Accurate Delivery of Mesenchymal Stem Cell Spheroids With Platelet‐Rich Fibrin Shield: Enhancing Survival and Repair Functions of Sp‐MSCs in Diabetic Wound Healing |
| title_sort | accurate delivery of mesenchymal stem cell spheroids with platelet rich fibrin shield enhancing survival and repair functions of sp mscs in diabetic wound healing |
| topic | cell spheroid diabetic wound mesenchymal stem cell platelet‐rich fiber ROS protection |
| url | https://doi.org/10.1002/advs.202413430 |
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