Accurate Delivery of Mesenchymal Stem Cell Spheroids With Platelet‐Rich Fibrin Shield: Enhancing Survival and Repair Functions of Sp‐MSCs in Diabetic Wound Healing

Accurate Delivery of Mesenchymal Stem Cell Spheroids With Platelet‐Rich Fibrin Shield: Enhancing Survival and Repair Functions of Sp‐MSCs in Diabetic Wound Healing

Abstract Diabetic wound is a significant clinical challenge, and stem cell therapy has shown great potential. This study explores the role of mesenchymal stem cell (MSC) spheroids (Sp‐MSCs) in healing diabetic wounds and the use of autologous plasma‐rich platelet fibrin (PRF) as a scaffold for Sp‐MS...

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Main Authors: Jinglve Zhang, Wenqing Xu, Yutian Xiao, Dingheng Su, Yusheng He, Huohong Yang, Yixin Xie, Xiaofang Wang, Ren‐He Xu, Shaorong Lei, Dingyu Wu
Format: Article
Language:English
Published: Wiley 2025-07-01
Series:Advanced Science
Subjects:
cell spheroid
diabetic wound
mesenchymal stem cell
platelet‐rich fiber
ROS protection
Online Access:https://doi.org/10.1002/advs.202413430
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Summary:Abstract Diabetic wound is a significant clinical challenge, and stem cell therapy has shown great potential. This study explores the role of mesenchymal stem cell (MSC) spheroids (Sp‐MSCs) in healing diabetic wounds and the use of autologous plasma‐rich platelet fibrin (PRF) as a scaffold for Sp‐MSCs. Through activation of the coagulation system, PRF offers a protective fibrin shield for Sp‐MSCs to promote the rapid recovery migration and proliferation of MSCs while maintaining the activity of Sp‐MSCs in an inflammatory overload environment by activating the related genes of Integrin‐β1‐vascular endothelial growth factor (VEGF), and Wnt/β‐catenin pathways. The inclusion of Sp‐MSCs accelerates the gelation of PRF and results in improved mechanical strength. Additionally, PRF enhances the repair function of Sp‐MSCs, creating a favorable microenvironment for angiogenesis. In the wound model of diabetic mice, the combination of PRF with Sp‐MSCs accelerates wound healing. Results show that this combination significantly promotes wound repair and regulates the immune microenvironment. The study suggests that PRF is a promising bio‐derived scaffold for stem cell applications in diabetic wounds, offering new directions for stem cell therapy and biomimetic scaffold material development.
ISSN:2198-3844

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