Purification, structural elucidation, and in vitro antitumor effects of novel polysaccharides from Bangia fuscopurpurea
A water-soluble polysaccharide, designated BFP-3, was isolated from Bangia fuscopurpurea by hot water extraction, anion-exchange, and size-exclusion chromatography and tested to determine its antitumor activity. The structural characteristics of BFP-3 were investigated by chemical and spectroscopic...
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Tsinghua University Press
2021-01-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453020301464 |
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| author | Jingna Wu Xiaoting Chen Kun Qiao Yongchang Su Zhiyu Liu |
| author_facet | Jingna Wu Xiaoting Chen Kun Qiao Yongchang Su Zhiyu Liu |
| author_sort | Jingna Wu |
| collection | DOAJ |
| description | A water-soluble polysaccharide, designated BFP-3, was isolated from Bangia fuscopurpurea by hot water extraction, anion-exchange, and size-exclusion chromatography and tested to determine its antitumor activity. The structural characteristics of BFP-3 were investigated by chemical and spectroscopic methods, including partial acid hydrolysis, methylation analysis, one- and two-dimensional nuclear magnetic resonance, and gas chromatography-mass spectrometry. The results showed that BFP-3 was mainly comprised of rhamnose, arabinose, mannose, glucose, and galactose. Moreover, the weight-average molecular weight of BFP-3 was estimated to be approximately 333 kDa. The backbone of BFP-3 was primarily composed of repeating 5-α-l-Araf-1→(4-α-d-Glcp-1)4→4,6-β-d-Manp-1 units, and the side chains consisted of repeating β-d-Galp-1→(4-β-d-Galp-1)4→4,6-β- d-Galp-1→3,4-α-l-Rhap, β-l-Arap-1→(3-β-d-Galp-1)3, and β-l-Arap-1 units. Counting Kit-8 assays revealed that BFP-3 significantly inhibited the proliferation of A2780, COC1, SKOV3, HO-8910, and OVCAR3 ovarian cancer cells in vitro, indicating that BFP-3 could have potential applications in the treatment of ovarian cancer. |
| format | Article |
| id | doaj-art-0330247940bc4392ab701d010cbd99e0 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-0330247940bc4392ab701d010cbd99e02025-02-03T05:11:53ZengTsinghua University PressFood Science and Human Wellness2213-45302021-01-011016371Purification, structural elucidation, and in vitro antitumor effects of novel polysaccharides from Bangia fuscopurpureaJingna Wu0Xiaoting Chen1Kun Qiao2Yongchang Su3Zhiyu Liu4Xiamen Key Laboratory of Marine Medicinal Natural Products Resources, Xiamen Medical College, 361023, Xiamen, China; Fujian Universities and Colleges Engineering Research Center of Marine Biopharmaceutical Resources, Xiamen Medical College, 361023, Xiamen, China; Corresponding author.Fisheries Research Institute of Fujian, 361013, Xiamen, ChinaFisheries Research Institute of Fujian, 361013, Xiamen, ChinaFisheries Research Institute of Fujian, 361013, Xiamen, ChinaFisheries Research Institute of Fujian, 361013, Xiamen, China; Corresponding author.A water-soluble polysaccharide, designated BFP-3, was isolated from Bangia fuscopurpurea by hot water extraction, anion-exchange, and size-exclusion chromatography and tested to determine its antitumor activity. The structural characteristics of BFP-3 were investigated by chemical and spectroscopic methods, including partial acid hydrolysis, methylation analysis, one- and two-dimensional nuclear magnetic resonance, and gas chromatography-mass spectrometry. The results showed that BFP-3 was mainly comprised of rhamnose, arabinose, mannose, glucose, and galactose. Moreover, the weight-average molecular weight of BFP-3 was estimated to be approximately 333 kDa. The backbone of BFP-3 was primarily composed of repeating 5-α-l-Araf-1→(4-α-d-Glcp-1)4→4,6-β-d-Manp-1 units, and the side chains consisted of repeating β-d-Galp-1→(4-β-d-Galp-1)4→4,6-β- d-Galp-1→3,4-α-l-Rhap, β-l-Arap-1→(3-β-d-Galp-1)3, and β-l-Arap-1 units. Counting Kit-8 assays revealed that BFP-3 significantly inhibited the proliferation of A2780, COC1, SKOV3, HO-8910, and OVCAR3 ovarian cancer cells in vitro, indicating that BFP-3 could have potential applications in the treatment of ovarian cancer.http://www.sciencedirect.com/science/article/pii/S2213453020301464Bangia fuscopurpureaPolysaccharideStructure, ovarian cancerAntitumor effect |
| spellingShingle | Jingna Wu Xiaoting Chen Kun Qiao Yongchang Su Zhiyu Liu Purification, structural elucidation, and in vitro antitumor effects of novel polysaccharides from Bangia fuscopurpurea Food Science and Human Wellness Bangia fuscopurpurea Polysaccharide Structure, ovarian cancer Antitumor effect |
| title | Purification, structural elucidation, and in vitro antitumor effects of novel polysaccharides from Bangia fuscopurpurea |
| title_full | Purification, structural elucidation, and in vitro antitumor effects of novel polysaccharides from Bangia fuscopurpurea |
| title_fullStr | Purification, structural elucidation, and in vitro antitumor effects of novel polysaccharides from Bangia fuscopurpurea |
| title_full_unstemmed | Purification, structural elucidation, and in vitro antitumor effects of novel polysaccharides from Bangia fuscopurpurea |
| title_short | Purification, structural elucidation, and in vitro antitumor effects of novel polysaccharides from Bangia fuscopurpurea |
| title_sort | purification structural elucidation and in vitro antitumor effects of novel polysaccharides from bangia fuscopurpurea |
| topic | Bangia fuscopurpurea Polysaccharide Structure, ovarian cancer Antitumor effect |
| url | http://www.sciencedirect.com/science/article/pii/S2213453020301464 |
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