Genetic Characterization of the Immortalized Human Nasopharyngeal Carcinoma Cell Line NPC/HK1
ABSTRACT Background Human nasopharyngeal carcinoma (NPC) cell lines are in vitro model systems that are widely available, easy to handle, and provide an unlimited supply of material. They also bypass ethical concerns associated with the use of primary human cells or tissue. However, many of these ce...
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Wiley
2025-02-01
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| Series: | Cancer Medicine |
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| Online Access: | https://doi.org/10.1002/cam4.70422 |
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| author | Anna Makowska Udo Kontny Josef vanHelden Barbara Hildebrandt Herdit M. Schüler Ralf Weiskirchen |
| author_facet | Anna Makowska Udo Kontny Josef vanHelden Barbara Hildebrandt Herdit M. Schüler Ralf Weiskirchen |
| author_sort | Anna Makowska |
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| description | ABSTRACT Background Human nasopharyngeal carcinoma (NPC) cell lines are in vitro model systems that are widely available, easy to handle, and provide an unlimited supply of material. They also bypass ethical concerns associated with the use of primary human cells or tissue. However, many of these cell lines including 5‐8F, 6‐10B, CNE‐1, CNE‐2, HNE‐1, HONE‐1, SUNE1, SUNE2, and NPC‐TW01 have been shown to be misidentified or cross‐contaminated. While simple molecular genotyping techniques such as short tandem repeat profiling of human cell lines are available to confirm cell line identity, scientists often do not implement strategies to avoid misidentification. This has resulted in a large volume of publications containing incorrect information. Methods In this paper, we have established a cell line karyogram that contains several marker chromosomes and a set of typical aberrations characteristic of NPC/HK1. Results and Conclusions Combined with the typical multiloci short tandem repeat signature of NPC/HK1, the cytogenetic analysis provides an effective means to avoid unreliable experimental outcomes and scientific misinterpretation. |
| format | Article |
| id | doaj-art-032c7886ff3c42299324caeb63fa1d64 |
| institution | OA Journals |
| issn | 2045-7634 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Wiley |
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| series | Cancer Medicine |
| spelling | doaj-art-032c7886ff3c42299324caeb63fa1d642025-08-20T02:22:09ZengWileyCancer Medicine2045-76342025-02-01143n/an/a10.1002/cam4.70422Genetic Characterization of the Immortalized Human Nasopharyngeal Carcinoma Cell Line NPC/HK1Anna Makowska0Udo Kontny1Josef vanHelden2Barbara Hildebrandt3Herdit M. Schüler4Ralf Weiskirchen5Division of Pediatric Hematology, Oncology and Stem Cell Transplantation University Hospital Aachen Aachen GermanyDivision of Pediatric Hematology, Oncology and Stem Cell Transplantation University Hospital Aachen Aachen GermanyInstitute of Molecular Pathobiochemistry, Experimental Gene Therapy and Clinical Chemistry (IFMPEGKC) University Hospital Aachen Aachen GermanyInstitute of Human Genetics Medical Faculty and University Hospital Düsseldorf, Heinrich Heine University Düsseldorf GermanyInstitute of Human Genetics Medical Faculty and University Hospital Düsseldorf, Heinrich Heine University Düsseldorf GermanyInstitute of Molecular Pathobiochemistry, Experimental Gene Therapy and Clinical Chemistry (IFMPEGKC) University Hospital Aachen Aachen GermanyABSTRACT Background Human nasopharyngeal carcinoma (NPC) cell lines are in vitro model systems that are widely available, easy to handle, and provide an unlimited supply of material. They also bypass ethical concerns associated with the use of primary human cells or tissue. However, many of these cell lines including 5‐8F, 6‐10B, CNE‐1, CNE‐2, HNE‐1, HONE‐1, SUNE1, SUNE2, and NPC‐TW01 have been shown to be misidentified or cross‐contaminated. While simple molecular genotyping techniques such as short tandem repeat profiling of human cell lines are available to confirm cell line identity, scientists often do not implement strategies to avoid misidentification. This has resulted in a large volume of publications containing incorrect information. Methods In this paper, we have established a cell line karyogram that contains several marker chromosomes and a set of typical aberrations characteristic of NPC/HK1. Results and Conclusions Combined with the typical multiloci short tandem repeat signature of NPC/HK1, the cytogenetic analysis provides an effective means to avoid unreliable experimental outcomes and scientific misinterpretation.https://doi.org/10.1002/cam4.70422cell authenticationCLASTREpstein–Barr virusICLACkaryotypingNPC |
| spellingShingle | Anna Makowska Udo Kontny Josef vanHelden Barbara Hildebrandt Herdit M. Schüler Ralf Weiskirchen Genetic Characterization of the Immortalized Human Nasopharyngeal Carcinoma Cell Line NPC/HK1 Cancer Medicine cell authentication CLASTR Epstein–Barr virus ICLAC karyotyping NPC |
| title | Genetic Characterization of the Immortalized Human Nasopharyngeal Carcinoma Cell Line NPC/HK1 |
| title_full | Genetic Characterization of the Immortalized Human Nasopharyngeal Carcinoma Cell Line NPC/HK1 |
| title_fullStr | Genetic Characterization of the Immortalized Human Nasopharyngeal Carcinoma Cell Line NPC/HK1 |
| title_full_unstemmed | Genetic Characterization of the Immortalized Human Nasopharyngeal Carcinoma Cell Line NPC/HK1 |
| title_short | Genetic Characterization of the Immortalized Human Nasopharyngeal Carcinoma Cell Line NPC/HK1 |
| title_sort | genetic characterization of the immortalized human nasopharyngeal carcinoma cell line npc hk1 |
| topic | cell authentication CLASTR Epstein–Barr virus ICLAC karyotyping NPC |
| url | https://doi.org/10.1002/cam4.70422 |
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