Proteomic Analysis of Bacillus thuringiensis Strain 4.0718 at Different Growth Phases
The growth process of Bacillus thuringiensis Bt4.0718 strain was studied using proteomic technologies. The proteins of Bt whole cells at three phases—middle vegetative, early sporulation, and late sporulation—were extracted with lysis buffer, followed with separation by 2-DE and identified by MALDI-...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/798739 |
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| author | Xiaohui Li Xuezhi Ding Liqiu Xia Yunjun Sun Can Yuan Jia Yin |
| author_facet | Xiaohui Li Xuezhi Ding Liqiu Xia Yunjun Sun Can Yuan Jia Yin |
| author_sort | Xiaohui Li |
| collection | DOAJ |
| description | The growth process of Bacillus thuringiensis Bt4.0718 strain was studied using proteomic technologies. The proteins of Bt whole cells at three phases—middle vegetative, early sporulation, and late sporulation—were extracted with lysis buffer, followed with separation by 2-DE and identified by MALDI-TOF/TOF MS. Bioactive factors such as insecticidal crystal proteins (ICPs) including Cry1Ac(3), Cry2Aa, and BTRX28, immune inhibitor (InhA), and InhA precursor were identified. InhA started to express at the middle vegetative phase, suggesting its contribution to the survival of Bt in the host body. At the early sporulation phase, ICPs started their expression. CotJC, OppA, ORF1, and SpoIVA related to the formation of crystals and spores were identified, the expression characteristics of which ensured the stable formation of crystals and spores. This study provides an important foundation for further exploration of the stable expression of ICPs, the smooth formation of crystals, and the construction of recombinant strains. |
| format | Article |
| id | doaj-art-030d053b68734c7798823f30abfda5a1 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-030d053b68734c7798823f30abfda5a12025-08-20T03:55:32ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/798739798739Proteomic Analysis of Bacillus thuringiensis Strain 4.0718 at Different Growth PhasesXiaohui Li0Xuezhi Ding1Liqiu Xia2Yunjun Sun3Can Yuan4Jia Yin5Key Laboratory of Microbial Molecular Biology of Hunan Province, College of Life Science, Hunan Normal University, Changsha 410081, ChinaKey Laboratory of Microbial Molecular Biology of Hunan Province, College of Life Science, Hunan Normal University, Changsha 410081, ChinaKey Laboratory of Microbial Molecular Biology of Hunan Province, College of Life Science, Hunan Normal University, Changsha 410081, ChinaKey Laboratory of Microbial Molecular Biology of Hunan Province, College of Life Science, Hunan Normal University, Changsha 410081, ChinaKey Laboratory of Microbial Molecular Biology of Hunan Province, College of Life Science, Hunan Normal University, Changsha 410081, ChinaKey Laboratory of Microbial Molecular Biology of Hunan Province, College of Life Science, Hunan Normal University, Changsha 410081, ChinaThe growth process of Bacillus thuringiensis Bt4.0718 strain was studied using proteomic technologies. The proteins of Bt whole cells at three phases—middle vegetative, early sporulation, and late sporulation—were extracted with lysis buffer, followed with separation by 2-DE and identified by MALDI-TOF/TOF MS. Bioactive factors such as insecticidal crystal proteins (ICPs) including Cry1Ac(3), Cry2Aa, and BTRX28, immune inhibitor (InhA), and InhA precursor were identified. InhA started to express at the middle vegetative phase, suggesting its contribution to the survival of Bt in the host body. At the early sporulation phase, ICPs started their expression. CotJC, OppA, ORF1, and SpoIVA related to the formation of crystals and spores were identified, the expression characteristics of which ensured the stable formation of crystals and spores. This study provides an important foundation for further exploration of the stable expression of ICPs, the smooth formation of crystals, and the construction of recombinant strains.http://dx.doi.org/10.1100/2012/798739 |
| spellingShingle | Xiaohui Li Xuezhi Ding Liqiu Xia Yunjun Sun Can Yuan Jia Yin Proteomic Analysis of Bacillus thuringiensis Strain 4.0718 at Different Growth Phases The Scientific World Journal |
| title | Proteomic Analysis of Bacillus thuringiensis Strain 4.0718 at Different Growth Phases |
| title_full | Proteomic Analysis of Bacillus thuringiensis Strain 4.0718 at Different Growth Phases |
| title_fullStr | Proteomic Analysis of Bacillus thuringiensis Strain 4.0718 at Different Growth Phases |
| title_full_unstemmed | Proteomic Analysis of Bacillus thuringiensis Strain 4.0718 at Different Growth Phases |
| title_short | Proteomic Analysis of Bacillus thuringiensis Strain 4.0718 at Different Growth Phases |
| title_sort | proteomic analysis of bacillus thuringiensis strain 4 0718 at different growth phases |
| url | http://dx.doi.org/10.1100/2012/798739 |
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