In Vivo Toxicity of Silver Nanoparticles and Silver Ions in Zebrafish (Danio rerio)

The influence of water chemistry on characterised polyvinyl pyrrolidone- (PVP-) coated silver nanoparticles (81 nm) was investigated. NaCl solution series of 100–800 mg L−1 lead to initial and temporal increase in nanoparticles size, but agglomeration was limited. pH variation (5–8) had only minor i...

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Main Authors: Katrine Bilberg, Mads Bruun Hovgaard, Flemming Besenbacher, Erik Baatrup
Format: Article
Language:English
Published: Wiley 2012-01-01
Series:Journal of Toxicology
Online Access:http://dx.doi.org/10.1155/2012/293784
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author Katrine Bilberg
Mads Bruun Hovgaard
Flemming Besenbacher
Erik Baatrup
author_facet Katrine Bilberg
Mads Bruun Hovgaard
Flemming Besenbacher
Erik Baatrup
author_sort Katrine Bilberg
collection DOAJ
description The influence of water chemistry on characterised polyvinyl pyrrolidone- (PVP-) coated silver nanoparticles (81 nm) was investigated. NaCl solution series of 100–800 mg L−1 lead to initial and temporal increase in nanoparticles size, but agglomeration was limited. pH variation (5–8) had only minor influence on the hydrodynamic particle size. Acute toxicity of nanosivler to zebrafish (Danio rerio) was investigated in a 48-hour static renewal study and compared with the toxicity of silver ions (AgNO3). The nanosilver and silver ion 48-hour median lethal concentration (LC50) values were 84 μg L−1 and 25 μg L−1, respectively. To investigate exposure-related stress, the fish behaviour was observed visually after 0, 3, 6, 12, 24, 27, 30, and 48 hours of both nanosilver and ionic silver treatments. These observations revealed increased rate of operculum movement and surface respiration after nanosilver exposure, suggesting respiratory toxicity. The present study demonstrates that silver nanoparticles are lethal to zebrafish.
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institution OA Journals
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publishDate 2012-01-01
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series Journal of Toxicology
spelling doaj-art-030651552459477a8d46f86e6aaec9662025-08-20T02:03:25ZengWileyJournal of Toxicology1687-81911687-82052012-01-01201210.1155/2012/293784293784In Vivo Toxicity of Silver Nanoparticles and Silver Ions in Zebrafish (Danio rerio)Katrine Bilberg0Mads Bruun Hovgaard1Flemming Besenbacher2Erik Baatrup3Interdisciplinary Nanoscience Center (iNANO) and Department of Physics and Astronomy, Aarhus University, 8000 Aarhus C, DenmarkInterdisciplinary Nanoscience Center (iNANO) and Department of Physics and Astronomy, Aarhus University, 8000 Aarhus C, DenmarkInterdisciplinary Nanoscience Center (iNANO) and Department of Physics and Astronomy, Aarhus University, 8000 Aarhus C, DenmarkSection of Zoophysiology, Department of Biological Sciences, Aarhus University, C.F. Moellers Allé 3, Building 1131, 8000 Aarhus C, DenmarkThe influence of water chemistry on characterised polyvinyl pyrrolidone- (PVP-) coated silver nanoparticles (81 nm) was investigated. NaCl solution series of 100–800 mg L−1 lead to initial and temporal increase in nanoparticles size, but agglomeration was limited. pH variation (5–8) had only minor influence on the hydrodynamic particle size. Acute toxicity of nanosivler to zebrafish (Danio rerio) was investigated in a 48-hour static renewal study and compared with the toxicity of silver ions (AgNO3). The nanosilver and silver ion 48-hour median lethal concentration (LC50) values were 84 μg L−1 and 25 μg L−1, respectively. To investigate exposure-related stress, the fish behaviour was observed visually after 0, 3, 6, 12, 24, 27, 30, and 48 hours of both nanosilver and ionic silver treatments. These observations revealed increased rate of operculum movement and surface respiration after nanosilver exposure, suggesting respiratory toxicity. The present study demonstrates that silver nanoparticles are lethal to zebrafish.http://dx.doi.org/10.1155/2012/293784
spellingShingle Katrine Bilberg
Mads Bruun Hovgaard
Flemming Besenbacher
Erik Baatrup
In Vivo Toxicity of Silver Nanoparticles and Silver Ions in Zebrafish (Danio rerio)
Journal of Toxicology
title In Vivo Toxicity of Silver Nanoparticles and Silver Ions in Zebrafish (Danio rerio)
title_full In Vivo Toxicity of Silver Nanoparticles and Silver Ions in Zebrafish (Danio rerio)
title_fullStr In Vivo Toxicity of Silver Nanoparticles and Silver Ions in Zebrafish (Danio rerio)
title_full_unstemmed In Vivo Toxicity of Silver Nanoparticles and Silver Ions in Zebrafish (Danio rerio)
title_short In Vivo Toxicity of Silver Nanoparticles and Silver Ions in Zebrafish (Danio rerio)
title_sort in vivo toxicity of silver nanoparticles and silver ions in zebrafish danio rerio
url http://dx.doi.org/10.1155/2012/293784
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AT madsbruunhovgaard invivotoxicityofsilvernanoparticlesandsilverionsinzebrafishdaniorerio
AT flemmingbesenbacher invivotoxicityofsilvernanoparticlesandsilverionsinzebrafishdaniorerio
AT erikbaatrup invivotoxicityofsilvernanoparticlesandsilverionsinzebrafishdaniorerio