Comment on “Organelles Ca²⁺ redistribution contributes to cadmium-induced EMT of renal cancer cells through p-cPLA2-mediated arachidonic acid release”
This commentary proposes enhancements to improve a study on cadmium-induced EMT in renal cancer cells. Key recommendations encompass the following aspects: Supplementing statistical power justification through explicit definition of parameters including ≥80% desired power, minimal effect size, and i...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-09-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651325010875 |
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| _version_ | 1849248226218082304 |
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| author | Jiaqi Liang Guangzhen Wu |
| author_facet | Jiaqi Liang Guangzhen Wu |
| author_sort | Jiaqi Liang |
| collection | DOAJ |
| description | This commentary proposes enhancements to improve a study on cadmium-induced EMT in renal cancer cells. Key recommendations encompass the following aspects: Supplementing statistical power justification through explicit definition of parameters including ≥80% desired power, minimal effect size, and intra-group variability; Report exact P values with confidence intervals to improve statistical transparency; Clarification of p38 MAPK versus cPLA2 pathway contributions via co-inhibition experiments; Exclusion of TRP channel confounding effects when using 2-APB through targeted TRPV6 inhibition; Distinction between direct Cd²⁺ effects and toxicity-induced compensatory responses; Provision of explicit rationale for selecting Mag-Fluo-4/AM and Rhod-2/AM fluorescent probes; Experimental verification of PMCA inhibition synergism with cadmium-induced cytosolic Ca²⁺ overload. These recommendations would enhance the robustness of the original study's conclusions. |
| format | Article |
| id | doaj-art-0303d7a891bf4bfab4fbb5cedd0dadee |
| institution | Kabale University |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-0303d7a891bf4bfab4fbb5cedd0dadee2025-08-20T03:57:56ZengElsevierEcotoxicology and Environmental Safety0147-65132025-09-0130311874210.1016/j.ecoenv.2025.118742Comment on “Organelles Ca²⁺ redistribution contributes to cadmium-induced EMT of renal cancer cells through p-cPLA2-mediated arachidonic acid release”Jiaqi Liang0Guangzhen Wu1First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning, ChinaCorrespondence to: Department of Urology, First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, China.; First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning, ChinaThis commentary proposes enhancements to improve a study on cadmium-induced EMT in renal cancer cells. Key recommendations encompass the following aspects: Supplementing statistical power justification through explicit definition of parameters including ≥80% desired power, minimal effect size, and intra-group variability; Report exact P values with confidence intervals to improve statistical transparency; Clarification of p38 MAPK versus cPLA2 pathway contributions via co-inhibition experiments; Exclusion of TRP channel confounding effects when using 2-APB through targeted TRPV6 inhibition; Distinction between direct Cd²⁺ effects and toxicity-induced compensatory responses; Provision of explicit rationale for selecting Mag-Fluo-4/AM and Rhod-2/AM fluorescent probes; Experimental verification of PMCA inhibition synergism with cadmium-induced cytosolic Ca²⁺ overload. These recommendations would enhance the robustness of the original study's conclusions.http://www.sciencedirect.com/science/article/pii/S0147651325010875Cadmium (Cd²⁺)Calcium (Ca²⁺)Plasma membrane Ca²⁺-ATPase（PMCA）Epithelial mesenchymal transition（EMT）Renal cell carcinoma |
| spellingShingle | Jiaqi Liang Guangzhen Wu Comment on “Organelles Ca²⁺ redistribution contributes to cadmium-induced EMT of renal cancer cells through p-cPLA2-mediated arachidonic acid release” Ecotoxicology and Environmental Safety Cadmium (Cd²⁺) Calcium (Ca²⁺) Plasma membrane Ca²⁺-ATPase（PMCA） Epithelial mesenchymal transition（EMT） Renal cell carcinoma |
| title | Comment on “Organelles Ca²⁺ redistribution contributes to cadmium-induced EMT of renal cancer cells through p-cPLA2-mediated arachidonic acid release” |
| title_full | Comment on “Organelles Ca²⁺ redistribution contributes to cadmium-induced EMT of renal cancer cells through p-cPLA2-mediated arachidonic acid release” |
| title_fullStr | Comment on “Organelles Ca²⁺ redistribution contributes to cadmium-induced EMT of renal cancer cells through p-cPLA2-mediated arachidonic acid release” |
| title_full_unstemmed | Comment on “Organelles Ca²⁺ redistribution contributes to cadmium-induced EMT of renal cancer cells through p-cPLA2-mediated arachidonic acid release” |
| title_short | Comment on “Organelles Ca²⁺ redistribution contributes to cadmium-induced EMT of renal cancer cells through p-cPLA2-mediated arachidonic acid release” |
| title_sort | comment on organelles ca²⁺ redistribution contributes to cadmium induced emt of renal cancer cells through p cpla2 mediated arachidonic acid release |
| topic | Cadmium (Cd²⁺) Calcium (Ca²⁺) Plasma membrane Ca²⁺-ATPase（PMCA） Epithelial mesenchymal transition（EMT） Renal cell carcinoma |
| url | http://www.sciencedirect.com/science/article/pii/S0147651325010875 |
| work_keys_str_mv | AT jiaqiliang commentonorganellesca2redistributioncontributestocadmiuminducedemtofrenalcancercellsthroughpcpla2mediatedarachidonicacidrelease AT guangzhenwu commentonorganellesca2redistributioncontributestocadmiuminducedemtofrenalcancercellsthroughpcpla2mediatedarachidonicacidrelease |