Plasma Levels of Matrix Metalloproteinases 2 and 9 in Patients with Chronic Chagas Heart Disease and Systemic Arterial Hypertension: Correlation with TGF-Beta Plasma Levels
Background. Chronic Chagas heart disease (CCHD) and systemic arterial hypertension (SAH) frequently coexists in areas where Chagas disease is endemic. The effects of the association of both conditions (CCHD-SAH) on the extracellular matrix (ECM) remodeling are unknown. Matrix metalloproteinases (MMP...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-01-01
|
| Series: | Cardiology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2023/8484697 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849305602702966784 |
|---|---|
| author | Reinaldo B. Bestetti Renata Dellalibera-Joviliano Ellen Rizzi Giselle F. Bonacio Milton Faria-Jr Rosemeire Furlan-Daniel Suzeley Castro-França |
| author_facet | Reinaldo B. Bestetti Renata Dellalibera-Joviliano Ellen Rizzi Giselle F. Bonacio Milton Faria-Jr Rosemeire Furlan-Daniel Suzeley Castro-França |
| author_sort | Reinaldo B. Bestetti |
| collection | DOAJ |
| description | Background. Chronic Chagas heart disease (CCHD) and systemic arterial hypertension (SAH) frequently coexists in areas where Chagas disease is endemic. The effects of the association of both conditions (CCHD-SAH) on the extracellular matrix (ECM) remodeling are unknown. Matrix metalloproteinases (MMP) 2 and 9 are involved in ECM remodeling. The aim of this study was to evaluate MMP 2 and MMP9 in CCHD-SAH patients and to correlate their levels with those of the profibrogenic cytokine TGF-beta. Methods. We included 19 patients with CCHD-SAH, 14 patients with CCHD alone, and 19 controls matched by sex and age. MMP-2 and MMP-9 plasma levels were studied by gel zymography and showed as optical densities (OD). TGF-beta plasma levels were measured by double-ligand ELISA and expressed as pg/mL. Results. Median (5th, 95th) MMP-2 plasma levels were 1224.7 OD (1160, 1433.5) in patients with CCHD alone, 1424.1 OD (1267.5, 1561) in patients with CCHD-SAH, and 940 OD (898.1, 1000.8) in controls (p=0.001). MMP-9 plasma levels were 1870 OD (1740, 1904.1) in patients with CCHD alone, 1754.6 OD (1650, 2049) in those with CCHD-SAH and 89.7 OD (80, 96) in controls (p=0.0003). MMP-9 plasma levels were higher than those of MMP 2 in patients with CCHD-SAH (p=0.01). No correlation was found between TGF-beta plasma levels with MMP-2 serum levels (r = 0.12; p=0.7), but a moderate negative correlation (r = −0.46; p=0.048) was observed between TGF-beta and MMP-9 plasma levels. Conclusions. MMP-2 and especially MMP-9 may play a role in the ECM remodeling process in patients with CCHD-SAH. TGF-Beta may counteract the MMP effect on the ECM remodeling process in patients with CCHD-SAH. |
| format | Article |
| id | doaj-art-02ed14c25e4243ee8cbd38cd83b2a50e |
| institution | Kabale University |
| issn | 2090-0597 |
| language | English |
| publishDate | 2023-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Cardiology Research and Practice |
| spelling | doaj-art-02ed14c25e4243ee8cbd38cd83b2a50e2025-08-20T03:55:23ZengWileyCardiology Research and Practice2090-05972023-01-01202310.1155/2023/8484697Plasma Levels of Matrix Metalloproteinases 2 and 9 in Patients with Chronic Chagas Heart Disease and Systemic Arterial Hypertension: Correlation with TGF-Beta Plasma LevelsReinaldo B. Bestetti0Renata Dellalibera-Joviliano1Ellen Rizzi2Giselle F. Bonacio3Milton Faria-Jr4Rosemeire Furlan-Daniel5Suzeley Castro-França6Department of MedicineDepartment of MedicineDepartment of BiotechnologyDepartment of BiotechnologyDepartment of MedicineDepartment of MedicineDepartment of BiotechnologyBackground. Chronic Chagas heart disease (CCHD) and systemic arterial hypertension (SAH) frequently coexists in areas where Chagas disease is endemic. The effects of the association of both conditions (CCHD-SAH) on the extracellular matrix (ECM) remodeling are unknown. Matrix metalloproteinases (MMP) 2 and 9 are involved in ECM remodeling. The aim of this study was to evaluate MMP 2 and MMP9 in CCHD-SAH patients and to correlate their levels with those of the profibrogenic cytokine TGF-beta. Methods. We included 19 patients with CCHD-SAH, 14 patients with CCHD alone, and 19 controls matched by sex and age. MMP-2 and MMP-9 plasma levels were studied by gel zymography and showed as optical densities (OD). TGF-beta plasma levels were measured by double-ligand ELISA and expressed as pg/mL. Results. Median (5th, 95th) MMP-2 plasma levels were 1224.7 OD (1160, 1433.5) in patients with CCHD alone, 1424.1 OD (1267.5, 1561) in patients with CCHD-SAH, and 940 OD (898.1, 1000.8) in controls (p=0.001). MMP-9 plasma levels were 1870 OD (1740, 1904.1) in patients with CCHD alone, 1754.6 OD (1650, 2049) in those with CCHD-SAH and 89.7 OD (80, 96) in controls (p=0.0003). MMP-9 plasma levels were higher than those of MMP 2 in patients with CCHD-SAH (p=0.01). No correlation was found between TGF-beta plasma levels with MMP-2 serum levels (r = 0.12; p=0.7), but a moderate negative correlation (r = −0.46; p=0.048) was observed between TGF-beta and MMP-9 plasma levels. Conclusions. MMP-2 and especially MMP-9 may play a role in the ECM remodeling process in patients with CCHD-SAH. TGF-Beta may counteract the MMP effect on the ECM remodeling process in patients with CCHD-SAH.http://dx.doi.org/10.1155/2023/8484697 |
| spellingShingle | Reinaldo B. Bestetti Renata Dellalibera-Joviliano Ellen Rizzi Giselle F. Bonacio Milton Faria-Jr Rosemeire Furlan-Daniel Suzeley Castro-França Plasma Levels of Matrix Metalloproteinases 2 and 9 in Patients with Chronic Chagas Heart Disease and Systemic Arterial Hypertension: Correlation with TGF-Beta Plasma Levels Cardiology Research and Practice |
| title | Plasma Levels of Matrix Metalloproteinases 2 and 9 in Patients with Chronic Chagas Heart Disease and Systemic Arterial Hypertension: Correlation with TGF-Beta Plasma Levels |
| title_full | Plasma Levels of Matrix Metalloproteinases 2 and 9 in Patients with Chronic Chagas Heart Disease and Systemic Arterial Hypertension: Correlation with TGF-Beta Plasma Levels |
| title_fullStr | Plasma Levels of Matrix Metalloproteinases 2 and 9 in Patients with Chronic Chagas Heart Disease and Systemic Arterial Hypertension: Correlation with TGF-Beta Plasma Levels |
| title_full_unstemmed | Plasma Levels of Matrix Metalloproteinases 2 and 9 in Patients with Chronic Chagas Heart Disease and Systemic Arterial Hypertension: Correlation with TGF-Beta Plasma Levels |
| title_short | Plasma Levels of Matrix Metalloproteinases 2 and 9 in Patients with Chronic Chagas Heart Disease and Systemic Arterial Hypertension: Correlation with TGF-Beta Plasma Levels |
| title_sort | plasma levels of matrix metalloproteinases 2 and 9 in patients with chronic chagas heart disease and systemic arterial hypertension correlation with tgf beta plasma levels |
| url | http://dx.doi.org/10.1155/2023/8484697 |
| work_keys_str_mv | AT reinaldobbestetti plasmalevelsofmatrixmetalloproteinases2and9inpatientswithchronicchagasheartdiseaseandsystemicarterialhypertensioncorrelationwithtgfbetaplasmalevels AT renatadellaliberajoviliano plasmalevelsofmatrixmetalloproteinases2and9inpatientswithchronicchagasheartdiseaseandsystemicarterialhypertensioncorrelationwithtgfbetaplasmalevels AT ellenrizzi plasmalevelsofmatrixmetalloproteinases2and9inpatientswithchronicchagasheartdiseaseandsystemicarterialhypertensioncorrelationwithtgfbetaplasmalevels AT gisellefbonacio plasmalevelsofmatrixmetalloproteinases2and9inpatientswithchronicchagasheartdiseaseandsystemicarterialhypertensioncorrelationwithtgfbetaplasmalevels AT miltonfariajr plasmalevelsofmatrixmetalloproteinases2and9inpatientswithchronicchagasheartdiseaseandsystemicarterialhypertensioncorrelationwithtgfbetaplasmalevels AT rosemeirefurlandaniel plasmalevelsofmatrixmetalloproteinases2and9inpatientswithchronicchagasheartdiseaseandsystemicarterialhypertensioncorrelationwithtgfbetaplasmalevels AT suzeleycastrofranca plasmalevelsofmatrixmetalloproteinases2and9inpatientswithchronicchagasheartdiseaseandsystemicarterialhypertensioncorrelationwithtgfbetaplasmalevels |