The Chinese herbal prescription Kang-Gong-Yan alleviates cervicitis by modulating metabolites and gut microbiota
Context Cervicitis is a common gynecological inflammatory disease. The Chinese herbal prescription Kang-Gong-Yan (KGY) is clinically effective against cervicitis; however, the chemical constituents and therapeutic mechanism of KGY remain elusive.Objective To analyze the chemical constituents of KGY...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Pharmaceutical Biology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/13880209.2024.2318791 |
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| author | Yanni Zhao Minyan Yuan Xiaodong Sun Pengjiao Wang Xiaoxia Meng Shuo Zhang Wei Luo Min Zhang Xiuli Gao |
| author_facet | Yanni Zhao Minyan Yuan Xiaodong Sun Pengjiao Wang Xiaoxia Meng Shuo Zhang Wei Luo Min Zhang Xiuli Gao |
| author_sort | Yanni Zhao |
| collection | DOAJ |
| description | Context Cervicitis is a common gynecological inflammatory disease. The Chinese herbal prescription Kang-Gong-Yan (KGY) is clinically effective against cervicitis; however, the chemical constituents and therapeutic mechanism of KGY remain elusive.Objective To analyze the chemical constituents of KGY and explore the potential mechanism of KGY in treating cervicitis.Materials and methods UHPLC-Q-Exactive Plus Orbitrap MS was used to identify the active compounds of KGY; Sprague-Dawley (SD) female rats were randomly divided into the control, model, and KGY groups. Phenol mucilage (25%) was slowly injected into the vagina and cervix of the rats to establish the cervicitis model. Then, rats in the KGY groups (low dose: 1 g/kg/d; medium dose: 5 g/kg/d; high dose: 10 g/kg/d) were continuously gavaged KGY for one week. HE staining was used to observe the cervical tissues of rats; ELISA was used to detect inflammatory factors in plasma; non-targeted metabolomics was used to analyze metabolites; 16S rRNA sequencing was used to analyze intestinal microorganisms.Results KGY exerted anti-cervicitis effects and decreased the levels of IL-6, IL-1β, and TNF-α. The mechanism of KGY in treating cervicitis is mainly associated with betaine, amino acid, pyrimidine, and phospholipid metabolism by regulating fifteen metabolites. Moreover, KGY reversed cervicitis-induced gut dysbiosis by mediating five bacteria.Discussion and conclusions The Chinese herbal prescription KGY may alleviate cervicitis by modulating metabolites and gut microbiota disorders. These findings provide a scientific basis for the clinical application of KGY and a new strategy for treating cervicitis in Chinese medicine. |
| format | Article |
| id | doaj-art-02ec45196ed04b47bc36c616e9aec37d |
| institution | OA Journals |
| issn | 1388-0209 1744-5116 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Pharmaceutical Biology |
| spelling | doaj-art-02ec45196ed04b47bc36c616e9aec37d2025-08-20T02:20:52ZengTaylor & Francis GroupPharmaceutical Biology1388-02091744-51162024-12-0162134135510.1080/13880209.2024.2318791The Chinese herbal prescription Kang-Gong-Yan alleviates cervicitis by modulating metabolites and gut microbiotaYanni Zhao0Minyan Yuan1Xiaodong Sun2Pengjiao Wang3Xiaoxia Meng4Shuo Zhang5Wei Luo6Min Zhang7Xiuli Gao8State Key Laboratory of Functions and Applications of Medicinal Plants and School of Pharmacy, Guizhou Medical University, Guiyang, ChinaState Key Laboratory of Functions and Applications of Medicinal Plants and School of Pharmacy, Guizhou Medical University, Guiyang, ChinaState Key Laboratory of Functions and Applications of Medicinal Plants and School of Pharmacy, Guizhou Medical University, Guiyang, ChinaState Key Laboratory of Functions and Applications of Medicinal Plants and School of Pharmacy, Guizhou Medical University, Guiyang, ChinaState Key Laboratory of Functions and Applications of Medicinal Plants and School of Pharmacy, Guizhou Medical University, Guiyang, ChinaExperimental Animal Center of Guizhou Medical University, Guiyang, ChinaGuizhou Huizheng Pharmaceutical Co., Ltd, Qiannan, ChinaState Key Laboratory of Functions and Applications of Medicinal Plants and School of Pharmacy, Guizhou Medical University, Guiyang, ChinaState Key Laboratory of Functions and Applications of Medicinal Plants and School of Pharmacy, Guizhou Medical University, Guiyang, ChinaContext Cervicitis is a common gynecological inflammatory disease. The Chinese herbal prescription Kang-Gong-Yan (KGY) is clinically effective against cervicitis; however, the chemical constituents and therapeutic mechanism of KGY remain elusive.Objective To analyze the chemical constituents of KGY and explore the potential mechanism of KGY in treating cervicitis.Materials and methods UHPLC-Q-Exactive Plus Orbitrap MS was used to identify the active compounds of KGY; Sprague-Dawley (SD) female rats were randomly divided into the control, model, and KGY groups. Phenol mucilage (25%) was slowly injected into the vagina and cervix of the rats to establish the cervicitis model. Then, rats in the KGY groups (low dose: 1 g/kg/d; medium dose: 5 g/kg/d; high dose: 10 g/kg/d) were continuously gavaged KGY for one week. HE staining was used to observe the cervical tissues of rats; ELISA was used to detect inflammatory factors in plasma; non-targeted metabolomics was used to analyze metabolites; 16S rRNA sequencing was used to analyze intestinal microorganisms.Results KGY exerted anti-cervicitis effects and decreased the levels of IL-6, IL-1β, and TNF-α. The mechanism of KGY in treating cervicitis is mainly associated with betaine, amino acid, pyrimidine, and phospholipid metabolism by regulating fifteen metabolites. Moreover, KGY reversed cervicitis-induced gut dysbiosis by mediating five bacteria.Discussion and conclusions The Chinese herbal prescription KGY may alleviate cervicitis by modulating metabolites and gut microbiota disorders. These findings provide a scientific basis for the clinical application of KGY and a new strategy for treating cervicitis in Chinese medicine.https://www.tandfonline.com/doi/10.1080/13880209.2024.2318791Kang-Gong-Yan soft capsulechemical compounds identificationmetabolomicsintestinal microorganisms |
| spellingShingle | Yanni Zhao Minyan Yuan Xiaodong Sun Pengjiao Wang Xiaoxia Meng Shuo Zhang Wei Luo Min Zhang Xiuli Gao The Chinese herbal prescription Kang-Gong-Yan alleviates cervicitis by modulating metabolites and gut microbiota Pharmaceutical Biology Kang-Gong-Yan soft capsule chemical compounds identification metabolomics intestinal microorganisms |
| title | The Chinese herbal prescription Kang-Gong-Yan alleviates cervicitis by modulating metabolites and gut microbiota |
| title_full | The Chinese herbal prescription Kang-Gong-Yan alleviates cervicitis by modulating metabolites and gut microbiota |
| title_fullStr | The Chinese herbal prescription Kang-Gong-Yan alleviates cervicitis by modulating metabolites and gut microbiota |
| title_full_unstemmed | The Chinese herbal prescription Kang-Gong-Yan alleviates cervicitis by modulating metabolites and gut microbiota |
| title_short | The Chinese herbal prescription Kang-Gong-Yan alleviates cervicitis by modulating metabolites and gut microbiota |
| title_sort | chinese herbal prescription kang gong yan alleviates cervicitis by modulating metabolites and gut microbiota |
| topic | Kang-Gong-Yan soft capsule chemical compounds identification metabolomics intestinal microorganisms |
| url | https://www.tandfonline.com/doi/10.1080/13880209.2024.2318791 |
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